- Tytuł:
- Enzymatic reductive amination of p-hydroxy- and phenylpyruvic acids as a method of synthesis of L-tyrosine and L-phenylalanine labelled with deuterium and tritium
- Autorzy:
-
Pałka, K.
Kańska, M. - Powiązania:
- https://bibliotekanauki.pl/articles/146609.pdf
- Data publikacji:
- 2012
- Wydawca:
- Instytut Chemii i Techniki Jądrowej
- Tematy:
-
deuterium
enzyme
L-phenylalanine
tritium
L-tyrosine - Opis:
- We report the synthesis of isotopomers of L-phenylalanine and L-tyrosine selectively labelled with hydrogen isotopes in the 2-position of the side chain. The deuterium or tritium label was introduced using reductive amination activity of enzyme L-phenylalanine dehydrogenase (EC 1.4.1.20). This way p-phenylpyruvic acid was converted into [2-2H]-, [2-3H]-, and doubly labelled [2-2H/3H]-isotopomers of L-phenylalanine, using deuteriated, tritiated, and mixed (DTO) incubation media, respectively. Similarly, p-hydroxyphenylpyruvic acid was converted into [2-2H]-, [2-3H]-, and [2-2H/3H]-L-tyrosine. Deuterium labelled isotopomers of L-phenylalanine and --tyrosine can be used as markers in the investigation of abnormal metabolism of these amino acids observed in patients with inborn genetic diseases such as phenylketonuria and tyrosinemia.
- Źródło:
-
Nukleonika; 2012, 57, 3; 383-387
0029-5922
1508-5791 - Pojawia się w:
- Nukleonika
- Dostawca treści:
- Biblioteka Nauki
Artykuł