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    Wyświetlanie 1-9 z 9
    Tytuł:
    Analysis of DNA transcription termination sequences of gene coding for phaC1 polymerase in Pseudomonas species
    Autorzy:
    Możejko, J.
    Królicka, M.
    Ciesielski, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/363160.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    polihydroksykwasy
    Pseudomonas
    analiza sekwencji
    terminator transkrypcji
    polyhydroxyalkanoates
    sequence analysis
    transcription terminator
    Opis:
    Polyhydroxyalkanoates (PHAs) are natural polyesters that are synthesized by many bacteria as an intracellular carbon and energy compound. Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) have gained much interest in research on microbial biopolymers because of their ease of chemical modification. Mcl-PHAs are naturally synthesized by Pseudomonas species by transformation of wide range of substrates. The physiological background of mcl-PHAs synthesis is known, and key genes engaged in this process are discovered already, but the knowledge about their molecular regulation is still limited. Especially, there is lack of information concerning the transcription termination of gene coding for PHA polymerase (phaC1). It is assumed that the main role could play Rho-independent termination, which is related to presence of palindromic sequences, which leads to formation of the hairpin structure and to dissociation of the ternary elongation complex (TEC). In this work, DNA sequences located after phaC1 gene belonging to nineteen Pseudomonas strains were investigated. Among all analyzed strains, five had palindromic sequences, typical for Rhoindependent terminators. Our results proved, that gene phaC1, coding for PHA polymerase, can be independently regulated only in some species.
    Źródło:
    Environmental Biotechnology; 2011, 7, 1; 12-16
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Substrate influence on the structure of methanogenic Archaea communities during anaerobic digestion
    Autorzy:
    Dąbrowska, D.
    Bułkowska, K.
    Ciesielski, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/363216.pdf
    Data publikacji:
    2015
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    anaerobic digestion
    Archaea community structure
    biogas
    PCR-DGGE
    fermentacja metanowa
    biogaz
    Opis:
    This study compares the diversity of methanogenic Archaeal communities that developed during biogas production in reactors fed with different substrates. Reactor I was fed with silages of maize and of alfalfa and timothy; and Reactor II was fed with these silages plus pig slurry and glycerol as co substrates. The Archaeal community structure was studied using polymerase chain reaction––denaturing gradient gel electrophoresis based on the 16S rRNA gene. In both reactors, Methanosphaerula palustris was most abundant, and species belonging to Methanolinea, Methanoculleus, and Methanotorris were present. Only Reactor I, where the ammonia concentration was lower, had species belonging to Methanospirillum and Methanosarcina. Thus, it appears that addition of pig slurry increased the ammonia concentration, which inhibited the growth of Methanospirillum and Methanosarcina.
    Źródło:
    Environmental Biotechnology; 2015, 11, 2; 41-47
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Analysis of ancient mitochondrial DNA of the Baltic Sea sturgeon (Acipenser sp.)
    Autorzy:
    Fopp-Bayat, D.
    Ciesielski, S.
    Luczyński, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/363174.pdf
    Data publikacji:
    2005
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    jesiotr
    historyczny mtDNA
    filogeneza molekularna
    gen cytochromu b
    Morze Bałtyckie
    Acipenser baeri
    Acipenser oxyrinchus
    Acipenser sturio
    ancient mtDNA
    cytochrome b gene
    molecular phylogeny
    Opis:
    Genetic relatedness between Baltic Sea sturgeon (Acipenser sturio L.) specimens caught in different geographic areas is not clear. According to previous studies, fish captured in different locations within the historic area of A. sturio habitation are genetically different to each other. We have examined a fragment (191 base pairs) of mitochondrial cytochrome b gene of four specimens of A. sturio found in Poland: three fish were preserved in museums of natural history and the bone of one fish was from an archaeological site. DNA sequences of the three museum samples were identical, whereas the DNA sequence of the archaeological sample differed in the 918 position of the cytochrome b gene. All the analyzed DNA fragments were similar to those of Acipenser baeri and genetically distant to Acipenser sturio and A. oxyrinchus.
    Źródło:
    Environmental Biotechnology; 2005, 1, 1; 29-33
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparison of PCR-DGGE and Nested-PCR-DGGE Approach for Ammonia Oxidizers Monitoring in Membrane Bioreactors’ Activated Sludge
    zalety i wady wykorzystywania techniki nested-PCR w monitoringu bakterii utleniających amoniak w osadzie czynnym bioreaktora membranowego
    Autorzy:
    Ziembińska-Buczyńska, A.
    Wiszniowski, J.
    Ciesielski, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/204755.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    ammonia oxidizing bacteria
    AOB
    16S rRNA gene
    Polymerase Chain Reaction – Denaturing Gradient Gel Electrophoresis
    PCR-DGGE
    nested-PCR
    utlenianie amoniaku
    bakteria utleniająca amoniak
    gen kodujący 16S rRNA
    Opis:
    Nitritation, the first stage of ammonia removal process is known to be limiting for total process performance. Ammonia oxidizing bacteria (AOB) which perform this process are obligatory activated sludge habitants, a mixture consisting of Bacteria, Protozoa and Metazoa used for biological wastewater treatment. Due to this fact they are an interesting bacterial group, from both the technological and ecological point of view. AOB changeability and biodiversity analyses both in wastewater treatment plants and lab-scale reactors are performed on the basis of 16S rRNA gene sequences using PCR-DGGE (Polymerase Chain Reaction – Denaturing Gradient Gel Electrophoresis) as a molecular biology tool. AOB researches are usually led with nested PCR. Because the application of nested PCR is laborious and time consuming, we have attempted to check the possibility of using only first PCR round to obtain DGGE fingerprinting of microbial communities. In this work we are comparing the nested and non-nested PCR-DGGE monitoring of an AOB community and presenting advantages and disadvantages of both methods used. The experiment revealed that PCR technique is a very sensitive tool for the amplification of even a minute amount of DNA sample. But in the case of nested-PCR, the sensitivity is higher and the template amount could be even smaller. The nested PCR-DGGE seems to be a better tool for AOB community monitoring and complexity research in activated sludge, despite shorter fragments of DNA amplification which seems to be a disadvantage in the case of bacteria identification. It is recommended that the sort of analysis approach should be chosen according to the aim of the study: nested-PCR-DGGE for community complexity analysis, while PCR-DGGE for identification of the dominant bacteria.
    Nitiritacja – pierwszy etap nitryfikacji, jest uznawany za krok limitujący przebieg całości procesu utleniania amoniaku. Bakterie utleniające amoniak (ang. ammonia oxidizing bacteria, AOB), które prowadzą ten proces są stałymi mieszkańcami osadu czynnego – mieszaniny bakterii, Protozoa i Metazoa, wykorzystywanych do biologicznego oczyszczania ścieków. Z tego powodu są one interesujące zarówno z punktu widzenia technologii, jak i ekologii mikroorganizmów. Analizy zmienności i bioróżnorodności bakterii utleniających amoniak, zarówno w oczyszczalni ścieków, jak i w reaktorach w skali laboratoryjnej, są prowadzone w oparciu o sekwencje genu kodującego 16S rRNA z użyciem metody biologii molekularnej, jaką jest PCR-DGGE (Polymerase Chain Reaction – Denaturing Gradient Gel Electrophoresis). Analizy te są zazwyczaj prowadzone techniką tzw. nested-PCR. Ze względu na fakt, że metoda ta wymaga większego nakładu pracy i czasu, niż tradycyjny jednoetapowy PCR (ang. non-nested PCR) podjęto próbę sprawdzenia możliwości zastosowania techniki jednoetapowego PCR do uzyskania wzorów prążkowych DGGE bakterii utleniających amoniak. W tej pracy zaprezentowano wyniki analizy PCR-DGGE z użyciem technik nested i non-nested PCR oraz podjęto próbę wykazania ich wad i zalet.
    Źródło:
    Archives of Environmental Protection; 2014, 40, 4; 31-38
    2083-4772
    2083-4810
    Pojawia się w:
    Archives of Environmental Protection
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cyclopenta[c]phenanthrene induction of CYP1A in brain of rainbow trout (Oncorhynchus mykiss)
    Autorzy:
    Brzuzan, P.
    Woźny, M.
    Łuczyński, M. K.
    Góra, M.
    Ciesielski, S.
    Kuźmiński, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/363272.pdf
    Data publikacji:
    2007
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    benzo(a)piren
    mózg
    CYP1A
    cyklopenta[c]fenantren
    absolutna kwantyfikacja mRNA
    pstrąg tęczowy
    benzo(a)pyrene
    brain
    cyclopenta[c]phenanthrene
    mRNA absolute quantification
    rainbow trout
    Opis:
    We assessed the effects of cyclopenta[c]phenanthrene (CP[c]Ph) and benzo[a]pyrene (B[a]P; positive control) on CYP1A gene expression in brain of juvenile rainbow trout (Oncorhynchus mykiss) using the quantitative reverse transcription polymerase chain reaction (Q-RT-PCR). A group of hatchery raised rainbow trout, with an average body mass of 49.4 g and total length of 15.5 cm were given an intraperitoneal injection (10 mg*kg-1) of either CP[c]Ph or B[a]P in corn oil (2 mg*mi-1 corn oil) or corn oil alone (control). After 24 and 48 h, trout brains were collected for mRNA isolation and analysis. After 24 hours of the exposure, only B[a]P-treated rainbow trout had 10-fold higher number of CYP1A transcripts (mean = 3.63*106 transcripts*µg-1 total RNA) than control fish (3.24*105 transcripts*µg-1 total RNA; Tukey test, P<0.05). After 48 hrs, significantly higher levels of CYP1A expression (Tukey test, P<0.001) were found in either CP[c]Ph- or B[a]P- induced group (1.45*106 and 6.92*106 transcriptsźµg-1 total RNA, respectively) over a control group (mean=1.41*105 transcripts*µg-1 total RNA). The finding that CYP1A in brain tissue was inducible by CP[c]Ph, a polycyclic aromatic hydrocarbon (PAH) of different than B[a]P planar characteristics, may further validate the use of rainbow trout brain CYP1A mRNA levels as a biomarker of PAH exposure.
    Źródło:
    Environmental Biotechnology; 2007, 3, 1; 10-14
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    cDNA fingerprint from the hepatopancreatic glands of pond snails (Lymnaea stagnalis) exposed to benzo[a]pyrene
    Autorzy:
    Woźny, M.
    Kowal, M.
    Ciesielski, S.
    Florczyk, M.
    Wiśniewski, R.
    Malicka, E.
    Brzuzan, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/363204.pdf
    Data publikacji:
    2014
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    bioindicator
    differential display
    expressed sequence tags
    polycyclic aromatic hydrocarbons
    bioindykator
    obraz zróżnicowanej ekspresji
    ekspresja fragmentów sekwencji
    policykliczne węglowodory aromatyczne
    Opis:
    Identification of differentially expressed genes that could be potentially used as biomarkers of PAH exposure of common invertebrate animal (like freshwater snail) would be a valuable resource for investigators interested in toxicology and biomonitoring of aquatic environments. Therefore, the aim of this research was to investigate effects of waterborne benzo[a]pyrene (B[a]P) exposure on mRNA expression in the pond snail’s (Lymnaea stagnalis) hepatopancreatic gland. Toward this end, mature individuals of pond snail (L. stagnalis) were treated with 50µM B[a]P solution in a short 36h static exposure test. Differential Display PCR (DD-PCR) was used to generate a unique cDNA fingerprint of genes that were differentially expressed in the tissues of exposed and unexposed snails. To assess the putative identity of the isolated cDNA amplicons (ESTs), BLAST queries were performed to find similarities in their nucleotide sequence. Real-Time qPCR analysis was used to verify the DD-PCR expression profile. Finally, an additional independent exposure study, including higher dose of B[a]P (100µM), was conducted to validate the expression of selected ESTs. BLAST revealed that only 3 out of 9 isolated ESTs had meaningful information on their putative nucleotide sequence identity. The highest similarity was scored for EST-A1, identified as the transcript of UAP-like protein, found to be up-regulated after B[a]P exposure. The original expression pattern that was observed in DD-PCR step was coherent with results of the qPCR verification for 3 out of 5 analyzed ESTs. However, changes in the ESTs expression were modest and the treatment with B[a]P resulted in significant down-regulation for only 1 unidentified fragment (EST-G42, almost 2-fold; p<0.05) when compared to untreated snails. Although no significant changes were observed for EST-A1 and EST-G42 in the validation study, their expression pattern was consistent with that obtained from DD-PCR. Surprisingly, EST-C5 remained in contrast to the DD-PCR part, but it showed significant down-regulation in group of snails exposed to 100µM B[a]P (3.5-fold; p<0.05). The obtained results show that diverse genes may be involved in the molecular response of the pond snail’s hepatopancreas to treatment with B[a]P. However, further research is needed to confirm the utility of the discovered EST as PAH biomarkers in biomonitoring practices with L. stagnalis as bioindicator species.
    Źródło:
    Environmental Biotechnology; 2014, 10, 1; 8-17
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Accelerating PageRank computations
    Autorzy:
    Wierzchoń, S. T.
    Kłopotek, M. A.
    Ciesielski, K.
    Czerski, D.
    Dramiński, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/206365.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Instytut Badań Systemowych PAN
    Tematy:
    Page rank
    power method
    Jacobi iterations
    Monte Carlo
    Opis:
    Different methods for computing PageRank vectors are analysed. Particularly, we note the opposite behavior of the power method and the Monte Carlo method. Further, a method of reducing the number of iterations of the power method is suggested.
    Źródło:
    Control and Cybernetics; 2011, 40, 2; 259-274
    0324-8569
    Pojawia się w:
    Control and Cybernetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Map Quality Measurements for GNG and SOM based Document Collection Maps
    Autorzy:
    Kłopotek, M. A.
    Wierzchoń, S. T.
    Ciesielski, K.
    Dramiński, M.
    Czerski, D.
    Kujawiak, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/92814.pdf
    Data publikacji:
    2006
    Wydawca:
    Uniwersytet Przyrodniczo-Humanistyczny w Siedlcach
    Tematy:
    Search Engines
    map of document collection
    Opis:
    The paper presents a proposal of a set of measures for comparison of maps of document collections as well as preliminary results concerning evaluation of their usefulness and expressive power.
    Źródło:
    Studia Informatica : systems and information technology; 2006, 1(7); 65-76
    1731-2264
    Pojawia się w:
    Studia Informatica : systems and information technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Carbon nanotubes functionalized by salts containing stereogenic heteroatoms as electrodes in their battery cells
    Autorzy:
    Zdanowska, S.
    Pyzalska, M.
    Drabowicz, J.
    Kulawik, D.
    Pavlyuk, V.
    Girek, T.
    Ciesielski, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/779468.pdf
    Data publikacji:
    2016
    Wydawca:
    Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
    Tematy:
    multi-walled carbon nanotubes (MWCNT)
    battery
    bromination
    charge transfer
    X-ray spectroscopy
    electronic structure
    Opis:
    This paper concentrates on electrochemical properties of groups of multi-walled carbon nanotubes (MWCNT) functionalized with substituents containing a stereogenic heteroatom bonded covalently to the surface of the carbon nanotube. This system was tested in Swagelok-type cells. The cells comprised a system (functionalized CNT with salts containing S and P atoms) with a working electrode, microfiber separators soaked with electrolyte solution, and a lithium foil counter/reference (commercial LiCoO2 ) electrode. The electrolyte solution was 1 M LiPF6  in propylene carbonate. Using standard techniques (cyclic voltammetry/chronopotentiometry), galvanostatic cycling was performed on the cells at room temperature with a CH Instruments Model 600E potentiostat/galvanostat electrochemical measurements. Methods of functionalization CNT were compared in terms of the electrochemical properties of the studied systems. In all systems, the process of charge/discharge was observed.
    Źródło:
    Polish Journal of Chemical Technology; 2016, 18, 4; 22-26
    1509-8117
    1899-4741
    Pojawia się w:
    Polish Journal of Chemical Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-9 z 9

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