Autor: Bartkowiak-Broda, I - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: A new RAPD marker identifying restorer lines for CMS ogura system
Nowy marker typu RAPD identyfikujacy linie restorery dla systemu CMS ogura
Autorzy:: Furguth, A
Bartkowiak-Broda, I.
Powiązania:: https://bibliotekanauki.pl/articles/834103.pdf
Data publikacji:: 2005
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Tematy:: oilseed rape
CMS-ogura system
restorer line
hybrid
winter oilseed rape
Brassica napus
random amplified polymorphic DNA
Rfo restorer gene
molecular marker
Źródło:: Rośliny Oleiste - Oilseed Crops; 2005, 26, 2; 595-602
1233-8273
Pojawia się w:: Rośliny Oleiste - Oilseed Crops
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Comparison of isozyme, RAPD and AFLP markers in genetic similarity assessment of CMS ogura F1 hybrids of winter oilseed rape (Brassica napus L.) parental lines
Autorzy:: Liersch, A.
Bocianowski, J.
Kozak, M.
Bartkowiak-Broda, I.
Powiązania:: https://bibliotekanauki.pl/articles/19138.pdf
Data publikacji:: 2013
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:: Isozyme, RAPD and AFLP markers were evaluated and compared for their ability to determine genetic similarity in a set of 18 parental lines of winter oilseed rape F1 hybrids developed using CMS ogura. Five isozyme systems, 64 RAPD starters and 23 EcoRI+3/MseI+3 AFLP primer combinations generated 597 polymorphic markers. These polymorphic fragments were chosen for estimation of genetic similarity. Of the total number of polymorphic products, polymorphic zymograms constituted only 3.0% of the markers, 57 RAPD primers 37.7%, and 23 AFLP primer combinations 59.3%. The size of RAPD polymorphic products ranged from 564 to 2100 bp. On average there were four amplified bands per primer, with 61.0% polymorphism. The AFLP polymorphic fragments ranged from 72 to 1352 bp in size. AFLP assays generated 15 bands per primer pair on average and detected roughly four times more bands than with RAPD analysis. The genetic similarity coefficients based on all marker data range from 0.52 to 0.84. The correlation of genetic similarities based on RAPD and AFLP markers was 0.58. Estimated genetic similarity based on isozyme data was not correlated with genetic similarity derived from the two DNA-based markers. The dendrogram constructed with the three types of markers taken together grouped all the winter oilseed rape parental lines into several similar clusters. The genomic distribution and frequency of the RAPD and AFLP markers can serve well as estimators of genetic similarity between parental lines of F1 CMS ogura hybrids.
Źródło:: Acta Biologica Cracoviensia. Series Botanica; 2013, 55, 1
0001-5296
Pojawia się w:: Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:: Biblioteka Nauki