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Wyświetlanie 1-4 z 4
Tytuł:
Intra-population genetic diversity of cultivated carrot (Daucus carota L.) assessed by analysis of microsatellite markers
Autorzy:
Maksylewicz, Anna
Baranski, Rafal
Powiązania:
https://bibliotekanauki.pl/articles/1039480.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
SSR
biodiversity
genetic structure
gene pool
Opis:
Intra-population variation of 18 cultivated carrot (Daucus carota L. ssp. sativus) populations of diverse origins was evaluated using codominant microsatellite (SSR) markers. Using 27 genomic and EST-derived SSR markers, 253 alleles were identified with a mean 9.4 alleles per marker. Most of the alleles (60.5%) were rare i.e., with the frequency ≤ 0.05 while only 3.95% of alleles occurred with frequency > 0.6. EST-derived SSR markers were less polymorphic than genomic SSR markers. Differences in allele occurrence allowed 16 out of 18 populations to be assigned to either the Western or Asian carrot gene pools with high probability. Populations could be also discriminated due to the presence of private alleles (25.3% of all alleles). Most populations had excess of alleles in the homozygous state indicating their inbreeding, although heterozygous loci were common in F1 hybrids. Genetic diversity was due to allelic variation among plants within populations (62% of total variation) and between populations (38%). Accessions originating from continental Asia and Europe had more allelic variants and higher diversity than those from Japan and USA. Also, allelic richness and variability in landraces was higher than in F1 hybrids and open-pollinated cultivars.
Źródło:
Acta Biochimica Polonica; 2013, 60, 4; 753-760
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A protocol for sonication-assisted Agrobacterium rhizogenes-mediated transformation of haploid and diploid sugar beet (Beta vulgaris L.) explants
Autorzy:
Klimek-Chodacka, Magdalena
Baranski, Rafal
Powiązania:
https://bibliotekanauki.pl/articles/1039318.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
genetic modification
hairy root
SAAT
ultrasound
Opis:
Hairy root cultures obtained after Agrobacterium rhizogenes-mediated genetic transformation can serve as a model system for studying plant metabolism and physiology, or can be utilized for the production of secondary metabolites. So far no efficient protocol of hairy root development in sugar beet has been publically released. In this work, two A. rhizogenes strains (A4T and LBA1334) carrying a binary vector pBIN-m-gfp5-ER or pCAMBIA1301 possessing gfp and uidA reporter genes were used to transform petiole explants of haploid and diploid sugar beet genotypes. Five treatment combinations of sonicated-assisted Agrobacterium-mediated transformation were compared. Hairy roots appeared on 0% to 54% of explants depending on the treatment combination used. The highest frequency was achieved when explants of a diploid genotype were sonicated for 15 s in the inoculum containing A. rhizogenes of OD600=0.5 and then co-cultured for three days. Using the same treatment combinations the explants of haploid genotypes developed hairy roots with the frequency ranging from 10% to 36%. Transformation efficiency was independent on the bacterial strain used. The results indicate that haploid sugar beet explants are amenable to transformation using A. rhizogenes, and that the efficiency of that process can be increased by applying short ultrasound treatment.
Źródło:
Acta Biochimica Polonica; 2014, 61, 1; 13-17
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cryptographically Strong Elliptic Curves of Prime Order
Autorzy:
Barański, Marcin
Gliwa, Rafał
Szmidt, Janusz
Powiązania:
https://bibliotekanauki.pl/articles/1844627.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Mersenne primes
elliptic curves
security requirements
search algorithm
Magma
Opis:
The purpose of this paper is to generate cryptographically strong elliptic curves over prime fields Fp, where p is a Mersenne prime, one of the special primes or a random prime. We search for elliptic curves which orders are also prime numbers. The cryptographically strong elliptic curves are those for which the discrete logarithm problem is computationally hard. The required mathematical conditions are formulated in terms of parameters characterizing the elliptic curves. We present an algorithm to generate such curves. Examples of elliptic curves of prime order are generated with Magma.
Źródło:
International Journal of Electronics and Telecommunications; 2021, 67, 2; 207-212
2300-1933
Pojawia się w:
International Journal of Electronics and Telecommunications
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Experimental Investigation on Indicated Pressure and Heat Release for Direct Hydrogen Injection in a Dual Fuel Diesel Engine
Autorzy:
Siadkowska, Ksenia
Barański, Grzegorz
Sochaczewski, Rafał
Wendeker, Mirosław
Powiązania:
https://bibliotekanauki.pl/articles/2201773.pdf
Data publikacji:
2022
Wydawca:
Stowarzyszenie Inżynierów i Techników Mechaników Polskich
Tematy:
diesel engine
direct hydrogen injection
dual fuel
combustion engine
Opis:
This paper presents the research results for a diesel and hydrogen fueled engine. The research object is a four-cylinder, four-stroke ADCR engine with a displacement of 2,636 cm3. In the experiments, glow plugs were replaced with compressed hydrogen injectors and a special adapter. Hydrogen was supplied directly into a combustion chamber using a test injector. A hydrogen dose in the tests was changed at selected test points and ranged from 0 to 160 dm3/min. The research were conducted at 1,500 rpm. A hydrogen injection start angle and maximum hydrogen dose were specified from the preliminary experiments. The following parameters were analyzed: indicated mean effective pressure, maximum pressure, crank angle of maximum cylinder pressure occurrence and heat release. The obtained results were statistically analyzed. The conducted analysis focused on determining whether there are significant differences between early and late injection and how these changes affect the measured parameters.
Źródło:
Advances in Science and Technology. Research Journal; 2022, 16, 3; 54--66
2299-8624
Pojawia się w:
Advances in Science and Technology. Research Journal
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-4 z 4

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