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    Wyświetlanie 1-7 z 7
    Tytuł:
    Adeno-associated virus vector-mediated gene delivery to the vasculature and kidney.
    Autorzy:
    Kapturczak, Matthias
    Chen, Sifeng
    Agarwal, Anupam
    Powiązania:
    https://bibliotekanauki.pl/articles/1041403.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    kidney
    vasculature
    viral vectors
    endothelium
    recombinant adeno-associated virus
    gene therapy
    Opis:
    Relatively successful elsewhere, gene delivery aimed at the vasculature and kidney has made very little progress. In the kidney, the hurdles are related to the unique structure–function relationships of this organ and in the blood vessels to a variety of, mostly endothelial, factors making the delivery of transgenes very difficult. Among gene-therapeutic approaches, most viral gene delivery systems utilized to date have shown significant practical and safety-related limitations due to the level and duration of recombinant transgene expression as well as their induction of a significant host immune response to vector proteins. Recombinant adeno-associated virus (rAAV) vectors appear to offer a vehicle for safe, long-term transgene expression. rAAV-based vectors are characterized by a relative non-immunogenicity and the absence of viral coding sequences. Furthermore, they allow for establishment of long-term latency without deleterious effects on the host cell. This brief review addresses problems related to transgene-delivery to kidney and vasculature with particular attention given to rAAV vectors. The potential for gene therapy as a strategy for selected renal and vascular diseases is also discussed.
    Źródło:
    Acta Biochimica Polonica; 2005, 52, 2; 293-299
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Report of the turnip aphid, Lipaphis erysimi (Kaltenbach, 1843) from Missouri, USA
    Autorzy:
    Adhab, M.A.
    Schoelz, J.E.
    Powiązania:
    https://bibliotekanauki.pl/articles/65607.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    turnip aphid zob.mustard aphid
    mustard aphid
    Lipaphis erysimi
    virus vector
    cauliflower mosaic virus
    Missouri
    USA
    Opis:
    The turnip aphid, Lipaphis erysimi (Kaltenbach, 1843), is one of the most destructive pests in the United States. It has been reported in 33 states, but had not been reported in the state of Missouri. In this study we report this species for the first time in Missouri.
    Źródło:
    Journal of Plant Protection Research; 2015, 55, 3
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparison of Myzus persicae [Sulz.] and Macrosiphum euphorbiae [Thom.] development on boxthorn [Lycium halimifolium Mill.]
    Autorzy:
    Hurej, M.
    Kukula, A.
    Barabas, I.
    Powiązania:
    https://bibliotekanauki.pl/articles/65674.pdf
    Data publikacji:
    2003
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Lycium halimifolium
    development
    life cycle
    age structure
    plant virus
    aphid virus vector
    peach potato aphid
    Myzus persicae
    aphid
    population
    Macrosiphum euphorbiae
    Źródło:
    Journal of Plant Protection Research; 2003, 43, 1
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cloning of a Rift Valley Fever fusion gene in a plant virus derived replicon vector
    Autorzy:
    Omosimua, Rebecca Oziohu
    Iyappan, Gowtham
    Obembe, Olawole
    Ogunkanmi, Adebayo
    Sathishkumar, Ramalingam
    Powiązania:
    https://bibliotekanauki.pl/articles/1193478.pdf
    Data publikacji:
    2021
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    DNA Cloning
    Escherichia coli DH5α cells
    PBYR2e3K2Mc-GFP
    PBYR2e3K2Mc-RVFV
    RVF disease
    RVFV fusion gene
    bean yellow dwarf derived vector
    emerging disease
    geminivirus vector
    zoonotic disease
    Opis:
    Rift Valley fever (RVF) disease is an emerging viral zoonotic disease caused by Rift Valley fever virus (RVFV). RVF disease is seemingly becoming more severe in endemic populations and in areas of new outbreak. RVF disease is listed by WHO as requiring urgent research and development attention. There is no therapeutics or licensed vaccine for human use in the case of an eventual outbreak. There is a need to clone RVFV genes in cloning vectors which may be useful to produce antigens in plant cells or may be tested directly as DNA vaccines. A synthesized Rift Valley fever virus fusion gene was cloned in a bean yellow dwarf virus derived replicon vector; PBYR2e3K2Mc-GFP by removing and replacing the GFP gene. The cloned PBYR2e3K2Mc-RVFV fusion gene was confirmed by PCR, restriction digestion and DNA sequencing. This cloned RVFV fusion gene in plant virus vector can be used for subsequent protein expression in plants cells or used directly tested as DNA vaccines in future studies.
    Źródło:
    World Scientific News; 2021, 158; 159-172
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection of barley- and wheat-specific forms of Wheat dwarf virus in their vector Psammotettix alienus by duplex PCR assay
    Autorzy:
    Trzmiel, K.
    Klejdysz, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/66781.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    wheat dwarf virus
    virus
    cereal crop
    detection
    barley
    Psammotettix alienus
    Hemiptera
    Auchenorrhyncha
    Cicadellidae
    leafhopper
    duplex polymerase chain reaction
    Opis:
    Wheat dwarf virus (WDV) has been one of the most common viruses on cereal crops in Poland in the last years. This single stranded DNA virus is transmitted by the leafhopper spec, Psammotettix alienus (Dahlb.) in a persistent manner. It induces yellowing and streaking of leaves, dwarfing or even death of infected plants. The presence of barley- and wheat-specific forms of WDV (WDV-B and WDV-W) and their vector were previously reported in the country, however the literature data did not include any information on the infectivity of the vector in Poland. A duplex polymerase chain reaction (PCR) procedure was developed and optimized for simultaneous detection and differentiation of both forms in the vector. Two sets of primers amplify 734 bp and 483 bp specific fragments for WDV-W and WDV-B, respectively. The results were verified by a sequencing method. The studies were carried out on insect samples collected in autumn from four different locations in Greater Poland. The results confirmed the presence of WDV-W in the tested samples. They also suggested the concomitant of both forms of the virus in the vector. Additional studies to determine virus-vector relationships should be undertaken.
    Źródło:
    Journal of Plant Protection Research; 2018, 58, 1
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Nematodes in the soil and roots of spring barley grown in crop rotation and long-term monoculture
    Nicienie w glebie i korzeniach jęczmienia jarego uprawianego w płodozmianie i monokulturze długookresowej
    Autorzy:
    Skwiercz, Andrzej T.
    Adamiak, Ewa
    Stefanovska, Tatiana
    Szelągowska, Patrycja
    Zatoń, Kinga
    Sobolewska, Magdalena
    Powiązania:
    https://bibliotekanauki.pl/articles/2216864.pdf
    Data publikacji:
    2018
    Wydawca:
    Politechnika Bydgoska im. Jana i Jędrzeja Śniadeckich. Wydawnictwo PB
    Tematy:
    nematode
    pathogenic fungi
    vector of cereal virus
    grzyby patogenne
    nicienie
    wektory wirusów zbóż
    Opis:
    Background. Species structure of plant parasitic nematode populations from the rhizosphere of spring barley grown in an 18-year-old crop rotation and in a 48-year-old monoculture were analyzed and compared. Material and methods. Plots were established in fields of spring barley grown in an 18-year-old crop rotation and in a 48-year-old monoculture. Four $1 m^2$ plots were located in each corner of each field. Four soil samples from each $1 m^2$ plot were taken by a pedestrian cane of a $3 cm$ section at a depth of $40 cm$ in the vicinity of the barley roots at start (BBCH 09) and a day after harvest (BBCH 99). Each soil sample weighed 1 kg and contained 50 g of fresh roots and spikes. The sample from each of the four $1 m^2$ plots was a replicate (and at the same time, the combination of the soil + roots + stems), hence the isolated nematode from each part of the sample was a set of nematodes associated with the host on $1 m^2$. Results. Populations of dominating species such as Bitylenchus dubius, Merlinius microdorus, Pratylenchus neglectus and Heterodera avenae became higher in the monoculture than in the crop rotation. H. avenae eggs and larvae were infected by pathogenic fungi in 50% of samples from the monoculture (vs. 60% of the cysts from the crop rotation), and 18–35% of Pratylenchus specimens were colonized by bacteria, mainly by Bacillus penetrans. Conclusion. The results illustrated nematological changes occurring in long-term cropping systems and provided additional information necessary to fight dangerous viral vectors for the examined cereal.
    W pracy przedstawiono wyniki badań struktury gatunków pasożytniczych populacji nicieni roślinnych z ryzosfery jęczmienia jarego uprawianego w 18-letnim płodozmianie i 48-letniej monokulturze. W każdym rogu obu pól znajdowały się cztery poletka o powierzchni $1 m^2$. Cztery próbki gleby z każdego poletka pobrano laską glebową o przekroju $3 cm$ na głębokości $40 cm$ w pobliżu korzeni jęczmienia na początku (BBCH 09) i dzień po zbiorach (BBCH 99). Każda próbka gleby ważyła 1 kg, z czego 50 g stanowiły świeże korzenie i kłosy. Każda próbka, z której wyizolowano nicienie, stanowi połączenie powtórzeń każdego wariantu doświadczenia (połączenie gleba + korzenie + łodygi). Populacje gatunków dominujących, takich jak Bitylenchus dubius, Merlinius microdorus, Pratylenchus neglectus i Heterodera avenae, są znacznie wyższe w monokulturze niż w płodozmianie. Jaja i larwy gatunku H. avenae zostały zainfekowane chorobotwórczymi grzybami w 60% próbek pochodzących z monokultury jęczmienia (w porównaniu z płodozmianem, którego infekcja dotyczyła 50% cyst). Z kolei bakterie, głównie Bacillus penetrans, skolonizowały nicienie z rodzaju Pratylenchus, co stanowiło przedział 18–35% pobranych próbek. Uzyskane wyniki obrazują zmiany nematologiczne zachodzące w długotrwałych systemach uprawy jęczmienia jarego. Dostarczają one dodatkowych informacji niezbędnych do podjęcia odpowiednich działań w celu zwalczania niebezpiecznych wektorów wirusowych dla badanego zboża.
    Źródło:
    Acta Scientiarum Polonorum. Agricultura; 2018, 17, 3; 115-124
    1644-0625
    Pojawia się w:
    Acta Scientiarum Polonorum. Agricultura
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Targeting site-specific chromosome integration.
    Autorzy:
    Nuno-Gonzalez, Patricia
    Chao, Hsu
    Oka, Kazuhiro
    Powiązania:
    https://bibliotekanauki.pl/articles/1041401.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    adeno-associated virus
    phage C31 integrase
    helper-dependent adenovirus
    hybrid vector
    viral vector
    gene therapy
    Opis:
    The concept of gene therapy was introduced with great promise and high expectations. However, what appeared simple in theory has not translated into practice. Despite some success in clinical trials, the research community is still facing an old problem: namely, the need for a vector that can deliver a gene to target cells without adverse events while maintaining a long-term therapeutic effect. Some of these challenges are being addressed by the development of hybrid vectors which meld two different viral systems to incorporate efficient gene delivery and large cloning capacity with site-specific integration. The two known systems that integrate genes into specific sites in mammalian genomes are the adeno-associated virus and phage integrases. Recent experiments with hybrid vectors incorporating both of these systems are encouraging. However, extensive research should be directed towards the safety and efficacy of this approach before it will be available for gene therapy.
    Źródło:
    Acta Biochimica Polonica; 2005, 52, 2; 285-291
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-7 z 7

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