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Wyszukujesz frazę "Mazurkiewicz, Alina" wg kryterium: Wszystkie pola


Wyświetlanie 1-2 z 2
Tytuł:
Expression of the gene encoding blood coagulation factor VIII without domain B in E. coli bacterial expression system
Autorzy:
Mazurkiewicz-Pisarek, Anna
Mazurkiewicz, Alina
Mikiewicz, Diana
Baran, Piotr
Ciach, Tomasz
Powiązania:
https://bibliotekanauki.pl/articles/16706242.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
factor VIII
hemophilia type A
recombinant coagulation factor VIII
prokaryotic expression system
E. coli
recombinant protein production system
Opis:
In this article, we have demonstrated the feasibility of generating an active form of recombinant blood coagulation factor VIII using an E. coli bacterial expression system as a potential treatment for hemophilia type A. Factor VIII (FVIII), an essential blood coagulation protein, is a key component of the fluid phase blood coagulation system. So far, all available recombinant FVIII formulations have been produced using eukaryotic expression systems. Mammalian cells can produce catalytically active proteins with all the necessary posttranslational modifications. However, cultivating such cells is time-consuming and highly expensive, and the amount of the obtained product is usually low. In contrast to eukaryotic cells, bacterial culture is inexpensive and allows the acquisition of large quantities of recombinant proteins in a short time. With this study, we aimed to obtain recombinant blood coagulation factor VIII using the E. coli bacterial expression system, a method not previously explored for this purpose. Our research encompasses the synthesis of blood coagulation factor VIII and its expression in a prokaryotic system. To achieve this, we constructed a prokaryotic expression vector containing a synthetic factor VIII gene, which was then used for the transformation of an E. coli bacterial strain. The protein expression was confirmed by mass spectrometry, and we assessed the stability of the gene construct while determining the optimal growth conditions. The production of blood coagulation factor VIII by the E. coli bacterial strain was carried out on a quarter-technical scale. We established the conditions for isolation, denaturation, and renaturation of the protein, and subsequently confirmed the activity of FVIII.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2023, 104, 3; 247-262
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Reduction of Greenhouse Gas Emissions by Replacing Fertilizers with Digestate
Autorzy:
Kowalczyk-Juśko, Alina
Pochwatka, Patrycja
Mazurkiewicz, Jakub
Pulka, Jakub
Kępowicz, Barbara
Janczak, Damian
Dach, Jacek
Powiązania:
https://bibliotekanauki.pl/articles/24201726.pdf
Data publikacji:
2023
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
biogas plant
anaerobic digestion
digestate
fertilizing
emission reduction
Opis:
Digestate from a biogas plant can be a valuable organic and mineral fertilizer. Quantitative proportions of cosubstrates used in three agricultural biogas plants in Poland were analyzed. The composition of digestates was examined and large differences in the content of macronutrients were found, especially N and K. On the basis of the factors used to calculate emissions from the production and use of artificial fertilizers, the greenhouse gas (GHG) reduction resulting from replacing mineral fertilizers with digestate was calculated. In terms of 1 Mg of fresh digestate, this reduction may not seem large, as it amounts to 27.9–61.6 kg of CO2 eq, but it should be taken into account that digestate contains little dry matter. The annual amount of digestate used on an area of 1 ha allows avoiding GHG emissions of 25.8–44.5 Mg CO2 eq.
Źródło:
Journal of Ecological Engineering; 2023, 24, 4; 312--319
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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