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Tytuł:
β-Glucan-Mediated Alleviation of NaCl Stress in Ocimum basilicum L. in Relation to the Response of Antioxidant Enzymes and Assessment DNA Marker
Autorzy:
Alhasnawi, Arshad Naji
Powiązania:
https://bibliotekanauki.pl/articles/124951.pdf
Data publikacji:
2019
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
NaCl-stress
beta-glucan
antioxidants
molecular markers
DNA variations
genetic stability
Opis:
Salinity is one of the most important abiotic stresses which can negatively affect the plant metabolic processes in the world. This can impact the plant production, either for economic or sustenance benefits. The salinity stress can cause many physiological and biochemical changes in the plants. β-glucans are important polysaccharides, which are present in the cell walls of various cereal grains. They protect the plant responses and occur in plant suspensions. In this study, the researchers attempted to investigate various physiological mechanisms and determine the role of the β-glucans in the NaCl-mediated stress conditions on the Ocimum basilicum L. seedlings. For this purpose, they carried out an experiment for assessing various shoot and root parameters along with the antioxidant enzyme activities, proline levels and the ISSR markers. When the seedlings were exposed to the NaCl stress conditions, they showed a significant decrease in the growth parameters and an increase in the antioxidant and proline levels compared to the control seedlings grown under normal saline conditions. On the other hand, the β-glucantreated seeds, when grown under the saline stress conditions, showed better growth parameters as well as high antioxidant enzyme activities and proline levels, compared to the control and NaCl-treated plants. Furthermore, a PCR analysis was carried out using the ISSR-marker technology, which could help in evaluating the DNA fingerprints and genetic variations in the plants. The results indicated that the exogenous application of the β-glucans could protect the antioxidant enzyme activities and protect the plants against the salinity stresses, without affecting the DNA-markers without affecting the genetic variations and could be a better choice for use in DNA-markers.
Źródło:
Journal of Ecological Engineering; 2019, 20, 8; 90-99
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The use of bulk segregant analysis to identify a RAPD marker linked to the Mla locus of barley
Autorzy:
Czembor, Paweł Cz.
Czembor, Jerzy H.
Powiązania:
https://bibliotekanauki.pl/articles/2198932.pdf
Data publikacji:
2004-06-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
barley
Blumeria graminis f. sp. hordei
bulked segregant analysis
DNA marker
Mla locus
RAPD
Opis:
Resistance to powdery mildew, Blumeria graminis f.sp. hordei, is a major goal of many barley breeding programs. Resistance conferred by genes located at Mla locus is commonly used by barley breeders for effective control of powdery mildew. The use of molecular markers may facilitate barley breeding for powdery mildew resistance. In this study, bulked segregant analysis (BSA) was used to determine random amplified polymorphic DNAs (RAPDs) markers linked to Mla locus. Thirty one homozygous (17 resistant and 14 susceptible) F3 families from a cross between variety Pallas and single plant line E 1059-1-1 carrying gene at Mla locus were used as plant material. A total of 385 random 10-mer primers were screened to identify polymorphism between the appropriate resistant and susceptible DNA bulks and parents in BSA analysis. Only one PCR marker OPAA3400 (primer sequence: 5’-TTAGCGCCCC-3’), amplified in polymerase chain reaction (PCR) proved close linkage and was positioned in distance of 10 cM from Mla locus with 5.0 LOD threshold.
Źródło:
Plant Breeding and Seed Science; 2004, 49; 41-49
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of anthropogenic pollution in river water based on the genetic diversity of Aeromonas Hydrophila
Ocena zanieczyszczenia antropogenicznego wody rzecznej na podstwawie zróżnicowania genetycznego Aeromonas Hydrophila
Autorzy:
Korzekwa, K.
Gołaś, I.
Harnisz, M.
Powiązania:
https://bibliotekanauki.pl/articles/204566.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
woda rzeczna
zanieczyszczenie antropogeniczne
zróżnicowanie genetyczne
marker wielolekooporności
Aeromonas hydrophila
river water
aquaculture
DNA marker
genetic diversity
Opis:
Aeromonas hydrophila is a valuable indicator of the quality of water polluted by sewage and pathogens that pose a risk for humans and cold-blooded animals, including fi sh. The main aim of this research was to evaluate anthropogenic pollution of river water based on genetic diversity of 82 A. hydrophila strains by means of RAPD, semi-random AP-PCR (ISJ) and the rep-BOX conservative repeats test. Genetic diversity of A. hydrophila was HT = 0.28 (SD = 0.02) for all DNA markers (RAPD, semi random and rep-BOX). None of the analyzed electrophoretic patterns was identical, implying that there were many sources of strain transmission. The presence of genes for aerolysin (aerA), hemolysin (ahh1) and the cytotoxic enzyme complex (AHCYTOGEN) was verifi ed for all tested strains, and drug resistance patterns for tetracycline, enrofl oxacin and erythromycin were determined. The most diverse A. hydrophila strains isolated from river water were susceptible to enrofl oxacine (HS = 0.27), whereas less diverse strains were susceptible to erythromycin (HS = 0.24). The presence of the multidrug resistance marker (ISJ4-25; 1100 bp locus) in the examined strains (resistant to three analyzed drugs) indicates that intensive fi sh cultivation affects the microbiological quality of river water.
Aeromonas hydrophila jest cennym wskaźnikiem jakości wody w przypadku zanieczyszczeń ściekami oraz mikroorganizmami względnie patogennymi dla człowieka i zwierząt zimnokrwistych, w tym ryb. Celem niniejszych badań była ocena zanieczyszczenia antropogenicznego na podstawie zróżnicowania genetycznego 82 szczepów A. hydrophila poprzez analizy RAPD, pół-przypadkowo amplifi kowanej klasy AP-PCR (ISJ) i konserwatywnego powtórzenia rep-BOX. Zróżnicowanie genetyczne A. hydrophila wyniosło HT = 0,28 (SD = 0,02) dla wszystkich markerów DNA (RAPD, pół-przypadkowe i rep-BOX). Wszystkie szczepy dla wszystkich markerów ujawniły indywidualny wzór elektroforetyczny, nie ujawniono jednego źródła rozprzestrzeniania się szczepów. U szczepów potwierdzono obecność genów aerolizyny (aerA), hemolizyny (ahh1) i kompleksu enzymów cytotoksycznych (AHCYTOGEN), jak również określono wzorzec oporności na tetracyklinę, enrofl oksacynę i erytromycynę. Najbardziej zróżnicowane okazały się szczepy A. hydrophila wrażliwe na enrofl oksacynę (HS = 0,27) a najmniej zróżnicowane były szczepy wrażliwe na erytromycynę (HS = 0,24). Wyselekcjonowany marker wielolekooporności (locus ISJ4-25, 1100 pz) obecny u szczepów (opornych na 3 rozpatrywane leki) świadczy o wpływie intensywnej hodowli ryb na jakość mikrobiologiczną wody rzecznej.
Źródło:
Archives of Environmental Protection; 2012, 38, 3; 41-50
2083-4772
2083-4810
Pojawia się w:
Archives of Environmental Protection
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular identification of blast resistance genes in rice genotypes using gene-specific markers
Autorzy:
Al-Daej, M.I.
Ismail, M.
Rezk, A.A.
El-Malky, M.M.
Powiązania:
https://bibliotekanauki.pl/articles/80189.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
molecular identification
resistance gene
rice genotype
Oryza sativa
DNA marker
single-nucleotide polymorphism
simple sequence repeat
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2019, 100, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of DNA markers against illegal logging as a new tool for the Forest Guard Service
Autorzy:
Nowakowska, J.A.
Powiązania:
https://bibliotekanauki.pl/articles/38611.pdf
Data publikacji:
2011
Wydawca:
Instytut Badawczy Leśnictwa
Tematy:
application
DNA marker
DNA structure
wood
molecular identification
Forest Guard Service
tree species
determination
DNA profile
polymerase chain reaction
Opis:
DNA markers are currently the most precise tool for forest tree species identification and can be used for comparative analyses of plant material. Molecular diagnosis of evidence and reference material is based on comparing the structure of DNA markers duplicated in the PCR reaction and estimation of the DNA profiles obtained in studied wood samples. For this purpose, the microsatellite DNA markers are the most suitable tool because of their high polymorphism and accurate detection of structural changes in the genome. The analysis of tree stump DNA profiles let avoid timely collection of data such as tree age, diameter, height and thickness, although such a piece of information may advantageous in wood identification process. For each examined tree species, i.e. Pinus sylvestris L., Picea abies (L.) Karst., Quercus robur L. and Q. petraea (Matt.) Liebl., Fagus sylvatica L., Betula pendula L., and Alnus glutinosa L., wood identification was possible via the DNA profiles established on a basis of minimum 4 microsatellite nuclear DNA loci, and at least one cytoplasmatic (mitochondrial or chloroplast) DNA marker. Determination of the DNA profiles provided fast and reliable comparison of genetic similarity between material of evidence (wood, needles, leaves, seeds) and material of reference (tree stumps) in the forest. This was done with high probability (approximately 98– 99%).
Źródło:
Folia Forestalia Polonica. Series A . Forestry; 2011, 53, 2
0071-6677
Pojawia się w:
Folia Forestalia Polonica. Series A . Forestry
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Species-specific chloroplast DNA polymorphism in the trnV-rbcL region in Pinus sylvestris and P. mugo
Autorzy:
Wachowiak, W
Baczkiewicz, A.
Celinski, K.
Prus-Glowacki, W.
Powiązania:
https://bibliotekanauki.pl/articles/41357.pdf
Data publikacji:
2004
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Scotch pine
Pinus sylvestris
Pinus mugo
dwarf pine
hybridization
DNA marker
mtDNA
trnV-rbcL region
chloroplast
DNA polymorphism
Opis:
Four cpDNA regions were analyzed with the use of PCR-RFLP technique and nucleotide sequences of two mtDNA regions were characterized in order to find P. sylvestris and P. mugo species specific markers useful for studies of the species hybridization. The difference in the restriction fragment patterns of trnV-rbcL region after digestion with MvaI endonuclease was detected. The analyses of the species representatives from various geographic regions revealed that the observed polymorphism is species specific. No differences have been disclosed in the analyzed trnS-trnT, trnK1-trnK2, trnC-trnD cpDNA regions. The P. sylvestris and P.mugo mtDNA sequences of orf25 and coxI regions proved to be identical.
Źródło:
Dendrobiology; 2004, 51; 67-72
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The preliminary stage of SCAR markers development for Ranunculus subgen. Batrachium
Autorzy:
Jopek, M.
Powiązania:
https://bibliotekanauki.pl/articles/80542.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
preliminary stage
SCAR molecular marker
Batrachium
aquatic plant
Ranunculaceae
aquatic environment
DNA marker
internal transcribed spacer
random amplified polymorphic DNA
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rumex thyrsiflorus Fingerh. – sex ratios among seedlings and explants cultured in vitro
Autorzy:
Dziedzic, K.
Cygan, M.
Mizia, P.
Kwolek, D.
Slesak, H.
Powiązania:
https://bibliotekanauki.pl/articles/81088.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
Rumex thyrsiflorus
Rumex acetosa
sex ratio
sex chromosome
seedling
explant
in vitro culture
chromosome
scanning electron microscopy
DNA marker
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Frequent D-loop polymorphism in mtDNA enables genotyping of 1400-year-old human remains from Merowingian graves
Autorzy:
Zeller, M
Mirghomizadeh, F.
Wehner, H.D.
Blin, N.
Powiązania:
https://bibliotekanauki.pl/articles/2042042.pdf
Data publikacji:
2000
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
X chromosome
ancient remains
amplification technique
Y chromosome
ancient DNA
mtDNA
remains
Merowingian culture
polymorphism
mitochondrial DNA
man
DNA marker
DNA extraction
DNA
Źródło:
Journal of Applied Genetics; 2000, 41, 4; 285-292
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic variation of Picea abies in southern Germany as determined using isozyme and STS markers
Autorzy:
Konnert, M
Powiązania:
https://bibliotekanauki.pl/articles/40969.pdf
Data publikacji:
2009
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
international conference
Europe
forest ecosystem
plant breeding
tree
Norway spruce
Picea abies
genetic variation
Germany
isoenzyme
Bavaria
DNA marker
plant genetics
provenance
Opis:
Over 50 populations of Norway spruce from Bavaria were analysed at 23 isozyme gene loci. The mean genetic distances between these populations were quite small. A geographical grouping could not be observed, and discrimination between provenances from high and low altitudes was not identifiable using this marker type, either. The only difference between spruce populations from South Bavaria and those from Northeast Bavaria is in the presence of some distinct rare alleles. The highest values for the genetic diversity were detected for spruce stands in Northeast Bavaria (Frankonian Forest). Using STS markers, further genes of the nuclear genome of Picea abies can be dealt with. The genetic differences found on the basis of ten STS markers between different Picea abies seed lots and/or seedling populations are generally 2-3 times greater than those found by means of isozyme gene markers. DNA markers turned out to be an appropriate and substantial addition or even more a suitable alternative to isozyme markers for analysing genetic variation and testing provenance identity. Their advantages consist in a markedly wider variation as well as in the enlarged genome segments investigated.
Źródło:
Dendrobiology; 2009, 61 Supplement
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A green light for plants
Autorzy:
Jasinski, M.
Powiązania:
https://bibliotekanauki.pl/articles/80884.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bioinformatics
climate change
demand
DNA marker
feed
food
food security
gene expression
global population
grain
green light
plant
plant biotechnology
plant genetics
plant science
worldwide consumption
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2011, 92, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Description of DNA analysis techniques and their application in oat (Avena L.) genome research
Charakterystyka technik analiz DNA oraz ich wykorzystanie w badaniach owsa (Avena L.)
Autorzy:
Okon, S.
Kowalczyk, K.
Powiązania:
https://bibliotekanauki.pl/articles/26845.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
DNA analysis technique
application
oat
Avena
genome
molecular marker
crossbreeding efficiency
genetic map
hexaploid
diploid
plant species
crown rust
powdery mildew
plant resistance
DNA marker
polymerase chain reaction
Opis:
DNA markers are used not only to estimate genetic similarity and distance but also to select and identify desirable forms, to assess the adjustment of breeding material, to confirm crossbreeding efficiency, to determine seed purity, and to identify the genes which determine important functional traits. In the case of oat, DNA markers were used to construct and increase the density of genetic maps both in hexaploid and diploid species. The development of markers for some important traits provides a fast selection of genotypes containing dwarf genes as well as the resistance genes to crown rust and powdery mildew. Numerous analyses of genetic similarity between different species belonging to the genus Avena which are currently carried out may contribute to explaining the process of evolution within this genus and may also explain the development of particular species of oat.
Markery DNA znalazły zastosowanie nie tylko w ocenie podobieństwa lub dystansu genetycznego, ale również w selekcji i identyfikacji pożądanych form, ocenie wyrównania materiałów hodowlanych, potwierdzaniu skuteczności krzyżowań, ocenie czystości materiału siewnego czy też do identyfikacji genów, warunkujących ważne cechy użytkowe. U owsa posłużyły one między innymi do konstrukcji i zagęszczenia map genetycznych zarówno gatunków heksaploidalnych jak i diploidalnych. Opracowanie markerów dla niektórych cech użytkowych pozwala na szybką selekcję genotypów zawierających geny karłowatości, odporności na rdzę koronową czy mączniaka prawdziwego. Natomiast prowadzone liczne analizy podobieństwa genetycznego różnych gatunków z rodzaju Avena mogą przyczynić się do wyjaśnienia ewolucji w obrębie tego rodzaju jak również mogą wyjaśnić powstawanie poszczególnych gatunków owsa.
Źródło:
Acta Agrobotanica; 2012, 65, 1
0065-0951
2300-357X
Pojawia się w:
Acta Agrobotanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessment of 8-oxo-7,8-dihydro-2′-deoxyguanosine as a marker of oxidative DNA damage in gasoline filling station attendants
Autorzy:
Beerappa, Ravichandran
Venugopal, Dhananjayan
Sen, Somnath
Ambikapathy, Mala
Rao, Rajmohan H.
Powiązania:
https://bibliotekanauki.pl/articles/2179055.pdf
Data publikacji:
2013-10-01
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
DNA damage
ELISA
petrol filling attendants
urinary 8-oxo-7
8-dihydro-2’-deoxyguanosine
Opis:
Objectives: The urinary excretion of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) was used as a biomarker of oxidative DNA damage. The urinary 8-oxodG levels in petrol filling station attendants (exposed) at various petrol bunks were estimated as well as in the unexposed (cashier) population. Materials and Methods: A total of 100 workers (79 petrol fillers and 21 cashiers) aged from 20 to 41 years participated in the study. An informed consent was taken from each participant. Information on personal habits and health was obtained through a questionnaire. After shifts, urine samples were collected analyzed for 8-oxodG using enzyme-linked immunosorbent assay (ELISA). Results: Fifty-three percent of workers were in the 21-30 years age group. The maximum level of 8-oxodG was observed in the age group ≥ 41 years and the minimum in the age group of 31-40 years. The maximum level of 8-oxodG was observed among those workers who had ≥ 21 years of experience. The concentrations of 8-oxodG were significantly higher in petrol fillers than those in cashiers (p < 0.05). Conclusions: Despite the conflicting results obtained in our study it was shown that 8-oxodG is related to chemical exposure. Further research is needed embracing a bigger number of participants to highlight the correlations between the exposure and the effects.
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2013, 26, 5; 780-789
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Integrated statistical and rule-mining techniques for dna methylation and gene expression data analysis
Autorzy:
Mallik, S.
Mukhopadhyay, A.
Maulik, U.
Powiązania:
https://bibliotekanauki.pl/articles/1396742.pdf
Data publikacji:
2013
Wydawca:
Społeczna Akademia Nauk w Łodzi. Polskie Towarzystwo Sieci Neuronowych
Tematy:
statistical analysis
gene marker
methylation
genetic algorithm
DNA
Opis:
For determination of the relationships among significant gene markers, statistical analysis and association rule mining are considered as very useful protocols. The first protocol identifies the significant differentially expressed/methylated gene markers, whereas the second one produces the interesting relationships among them across different types of samples or conditions. In this article, statistical tests and association rule mining based approaches have been used on gene expression and DNA methylation datasets for the prediction of different classes of samples (viz., Uterine Leiomyoma/class-formersmoker and uterine myometrium/class-neversmoker). A novel rule-based classifier is proposed for this purpose. Depending on sixteen different rule-interestingness measures, we have utilized a Genetic Algorithm based rank aggregation technique on the association rules which are generated from the training set of data by Apriori association rule mining algorithm. After determining the ranks of the rules, we have conducted a majority voting technique on each test point to estimate its class-label through weighted-sum method. We have run this classifier on the combined dataset using 4-fold cross-validations, and thereafter a comparative performance analysis has been made with other popular rulebased classifiers. Finally, the status of some important gene markers has been identified through the frequency analysis in the evolved rules for the two class-labels individually to formulate the interesting associations among them.
Źródło:
Journal of Artificial Intelligence and Soft Computing Research; 2013, 3, 2; 101-115
2083-2567
2449-6499
Pojawia się w:
Journal of Artificial Intelligence and Soft Computing Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
DNA fingerprints generated by R.18.1 DNA probe in two stocks of chickens: Green Legged Patridgenous [GLP] and Rhode Island Red [RIR]
Autorzy:
Rosochacki, S J
Hillel, J
Jaszczak, K
Zawadzka, M
Powiązania:
https://bibliotekanauki.pl/articles/2046824.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
multi-locus DNA probe
hybridization
animal breeding
poultry
molecular marker
DNA
chicken
Opis:
Hybridization of a multi-locus DNA probe, R 18.1, to genomic DNA from poultry showed a highly polymorphic fingerprint pattern. The detected DNA fingerprints are individually specific and differ between Green Legged Patridgenous (GLP) and Rhode Island Red (RIR) stock of chickens. The average numbers of detected bands in RIR were 18.68 and in GLP - 15.33, but the average band sharing levels were 0.619 and 0.431, respectively. The level of polymorphism may be connected possibly with a higher level of inbreeding in the examined stock of chicken.
Źródło:
Journal of Applied Genetics; 1997, 38, 2; 173-178
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł

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