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    Wyświetlanie 1-6 z 6
    Tytuł:
    Comparative molecular analysis of strains of the Aleutian Disease Virus isolated from farmed and wild mink
    Autorzy:
    Czerwonka, Maciej
    Kowalczyk, Marek
    Kostro, Krzysztof
    Jeżewska-Witkowska, Grażyna
    Powiązania:
    https://bibliotekanauki.pl/articles/989585.pdf
    Data publikacji:
    2017
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    AMDV
    VP2 protein
    mink
    molecular phylogenetics
    Opis:
    Introduction and objective. Aleutian Disease is a significant biological factor causing substantial losses in mink farming. The virus inducing the disease also infects wild populations which may constitute an asymptomatic reservoir. To compare genetic variants of the AMD virus occurring in wild and farmed mink populations, an analysis was performed on a fragment of the VP2 protein sequence of the virus infecting both populations, taken from different living environments. Material and method. Genetic material was isolated from 11 farmed animals in which anti-AMDV antibodies had been detected and from 20 wild animals. The DNA obtained was amplified using primers specific for the fragment encoding the VP2 protein. The product obtained was sequenced and bioinformatic analysis was performed. Results. Viral material was detected in 11 farmed and 7 free-living animals. Similarity of sequences averaged 99% within groups and 94% between groups. The sequencing results made it possible to identify characteristic changes for each group. In the isolates from the wild animals, the following changes were observed in the epitope region with respect to the reference sequence: C3704T, G3710A, T3722C, T3746C and A3749G. In the isolates from the farmed animals a G3779A transition was noted. Phylogenetic analysis showed that the variants infecting the two groups occupy separate branches of the phylogenetic tree. Conclusion. The variants of the virus infecting the two groups may have a common origin, but at present they constitute two separate groups, with characteristic differences making it possible to recognize their genotype.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2017, 24, 3
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Tubulinosema loxostegi sp. n. (Microsporidia: Tubulinosematidae) from the Beet Webworm Loxostege sticticalis L. (Lepidoptera: Crambidae) in Western Siberia
    Autorzy:
    MALYSH, Julia M.
    TOKAREV, Yuri S.
    SITNICOVA, Natalia V.
    MARTEMYANOV, Vyacheslav V.
    FROLOV, Andrei N.
    ISSI, Irma V.
    Powiązania:
    https://bibliotekanauki.pl/articles/763407.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Beet webworm, microsporidia, taxonomy, molecular phylogenetics, Tubulinosema
    Opis:
    Adults of beet webworm Loxostege sticticalis were collected in Western Siberia in 2009 and 2010. A microsporidium was found infecting 12 of 50 moths in 2010. The parasite develops in direct contact with host cell cytoplasm, sporogony is presumably disporoblastic. The spores are ovoid, diplokaryotic, 4.2 × 2.4 μm in size (fresh), without a sporophorous vesicle. Electron microscopy showed: (a) tubules on the surface of sporoblasts and immature spores; (b) slightly anisofilar polar tube with 10–14 coils, last 2–3 coils of lesser electron density; (c) bipartite polaroplast with anterior and posterior parts composed of thin and thick lamellae, respectively; (d) an indentation in the region of the anchoring disc; (e) an additional layer of electron-dense amorphous matter on the exospore surface. The spore ultrastructure is characteristic of the genus Tubulinosema. Sequencing of small subunit and large subunit ribosomal RNA genes showed 98–99.6% similarity of this parasite to the Tubulinosema species available on Genbank. A new species Tubulinosema loxostegi sp. n. is established.
    Źródło:
    Acta Protozoologica; 2013, 52, 4
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Photosynthetic performance in Antarctic lichens with different growth forms reflect the diversity of lichenized algal adaptation to microhabitats
    Autorzy:
    Cao, Shunan
    Zhang, Jie
    Zheng, Hongyuan
    Liu, Chuanpeng
    Zhou, Qiming
    Powiązania:
    https://bibliotekanauki.pl/articles/2049638.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Antarctic
    lichens
    lichenized
    molecular phylogenetics
    photosynthesis
    Trebouxia
    Źródło:
    Polish Polar Research; 2015, 2; 175-188
    0138-0338
    2081-8262
    Pojawia się w:
    Polish Polar Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection of Prorocentrum shikokuense in the Mediterranean Sea and evidence that P. dentatum, P. obtusidens and P. shikokuense are three different species (Prorocentrales, Dinophyceae)
    Autorzy:
    Gómez, Fernando
    Zhang, Huan
    Roselli, Leonilde
    Lin, Senjie
    Powiązania:
    https://bibliotekanauki.pl/articles/2041940.pdf
    Data publikacji:
    2022-02-14
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Dinophyta
    harmful algae bloom
    invasive alien species
    non-indigenous species
    molecular phylogenetics
    toxic phytoplankton
    Opis:
    A dinoflagellate identified as Prorocentrum dentatum, P. shikokuense or P. donghaiense is responsible of massive harmful events. Blooms of a species identified as P. shikokuense have been recently reported in the Mediterranean Sea, and an exotic origin, tentatively introduced by ballast waters from Asia, has been hypothesized. The molecular data based on the small-, large subunit, and internal transcriber spacers ribosomal RNA gene (SSU-, LSU-, ITS rRNA) sequences confirmed P. shikokuense in the Mediterranean Sea. The Mediterranean ribotype is identical to a subtropical North Atlantic ribotype, and with slight divergence from the numerous sequences from the Pacific Ocean. To revisit the relationship between P. shikokuense (=P. donghaiense) and P. obtusidens, we provide the first micrographs of P. dentatum and P. obtusidens, the latter collected from the type locality. Our observations indicate that P. dentatum, P. obtusidens, and P. shikokuense are three different species. Their diagnostic morphological characters are: Prorocentrum dentatum is 44–60 μm long, leaf-shaped, pointed and central posterior end, conspicuous anterior shoulder; P. obtusidens is 33–41 μm long, irregular parallelepiped, almost paralel valve margins, eccentric and pointed posterior end, moderate anterior shoulder; and P. shikokuense is <25 μm long, sunflower seed-shaped, round and centric posterior end, inconspicuous anterior shoulder.
    Źródło:
    Acta Protozoologica; 2021, 60; 47-59
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Isospora dendrocinclae n. sp. (Apicomplexa: Eimeriidae) from the Whitechinned Woodcreeper (Dendrocincla merula) from South America
    Autorzy:
    McQuistion, Thomas E.
    Galewsky, Samuel
    Capparella, Angelo P.
    Rebling, Fredrick
    Powiązania:
    https://bibliotekanauki.pl/articles/763497.pdf
    Data publikacji:
    2010
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Isospora dendrocinclae, Passeriformes, Dendrocolaptidae, phylogenetics, ribosomal RNA, molecular systematics
    Opis:
    A new species of Isospora is described from the fecal contents of the white-chinned woodcreeper, Dendrocincla merula merula from Guyana and Dendrocincla merula barletti from Peru. Sporulated oocysts are subspherical to ovoid, 19.2 × 16.5 (15–23 × 14.5–19) μm, with a smooth, colorless, bilayered wall. The average shape index is 1.2. No micropyle or oocyst residuum are present, but the oocysts contain one polar granule. Sporocysts are ovoid, 12.9 × 8.3 (12–14 × 7–10) μm, average shape index of 1.7 with a smooth, single layered wall and composed of a small, knoblike Stieda body and a slightly larger, bubble shaped substieda body. The two sporocysts each contain a compact residuum composed of coarse, non-uniform granules and four randomly arranged, vermiform sporozoites each with a terminal refractile body and a centrally located nucleus. DNA sequences representing ITS-1 and ITS-4 regions of the 5.8S rDNA gene from the two isolates were amplified and compared. In addition to the two isolates showing similar morphological characteristics, they also had identical nucleotide sequences for the ITS-1 and ITS-4 regions of the 5.8S ribosomal gene.
    Źródło:
    Acta Protozoologica; 2010, 49, 2
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Principles and applications of Ligation Mediated PCR methods for DNA-based typing of microbial organisms
    Autorzy:
    Krawczyk, Beata
    Kur, Józef
    Stojowska-Swędrzyńska, Karolina
    Śpibida, Marta
    Powiązania:
    https://bibliotekanauki.pl/articles/1038839.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    rapid methods
    molecular epidemiology
    genotyping
    microbial phylogenetics
    PCR (polymerase chain reaction)
    Opis:
    A significant number of DNA-based techniques has been introduced into the field of microorganisms' characterization and taxonomy. These genomic fingerprinting methods were developed to detect DNA sequence polymorphisms by using general principles, such as restriction endonuclease analysis, molecular hybridization, and PCR amplification. In recent years, some alternative techniques based on ligation of oligonucleotide adapters before DNA amplification by PCR, known as Ligation-Mediated PCR methods (LM PCR), have been successfully applied for the typing of microorganisms below the species level. These molecular methods include: Amplified Fragment Length Polymorphism (AFLP), Amplification of DNA fragments Surrounding Rare Restriction Sites (ADSRRS), PCR Melting Profiles (PCR MP), Ligation Mediated PCR/Shifter (LM PCR/Shifter), Infrequent-Restriction-Site Amplification (IRS PCR), double digestion Ligation Mediated Suppression PCR (ddLMS PCR). These techniques are now applied more and more often because they involve less time, are comparably inexpensive, and require only standard lab equipment. Here, we present a general review of this group of methods showing their possibilities and limitations. We also identify questions and propose solutions which may be helpful in choosing a particular LM PCR method for the achievement of the required goal.
    Źródło:
    Acta Biochimica Polonica; 2016, 63, 1; 39-52
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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