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Tytuł:
Incubator for individual eggs of fish
Autorzy:
Bardega, R.
Luczynski, M.
Powiązania:
https://bibliotekanauki.pl/articles/363084.pdf
Data publikacji:
2007
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
jakość ikry
inkubacja
przeżywalność
reprodukcja ryb
egg quality
incubation
survival
fish reproduction
Opis:
We have constructed and tested a device for incubation of individual eggs of fish. We measured the diameter of fertilized and water hardened eggs of vendace (Coregonus albula). Each egg developed in a separate incubator, which was a modified Pasteur pipette. Incubation of 315 eggs resulted in 249 hatched embryos (eleutheroembryos). The technique enabled identification of each eleutheroembryo hatching from a given egg. Example of relationship between external diameter of egg and the length of eleutheroembryo showed potential applications of the technique in studies on fish reproduction biology.
Źródło:
Environmental Biotechnology; 2007, 3, 2; 49-51
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biotechnology in freshwater finfish aquaculture
Autorzy:
Luczynski, M.
Ocalewicz, K.
Powiązania:
https://bibliotekanauki.pl/articles/363230.pdf
Data publikacji:
2007
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
akwakultura
biotechnologia
inżynieria genetyczna
pstrąg tęczowy
aquaculture
fish
biotechnology
genetic engineering
rainbow trout
Źródło:
Environmental Biotechnology; 2007, 3, 2; 37-38
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microsatellite DNA polymorphism in sturgeon species and their hybrids reared in Polish aquaculture farms
Autorzy:
Fopp-Bayat, D.
Luczynski, M.
Powiązania:
https://bibliotekanauki.pl/articles/363194.pdf
Data publikacji:
2006
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
jesiotr
identyfikacja gatunkowa
DNA mikrosatelitarne
hybrydy
polimorfizm
hybrids
microsatellite DNA
polymorphism
PCR
species identification
sturgeons
Opis:
Highly variable microsatellite DNA loci show high levels of variation per locus and provide molecular markers for species and for populations of small effective size. In this study we applied microsatellite markers to identify specimens belonging to several sturgeon species and their interspecific hybrids. Nine microsatellite loci (Afu-19, Afu-22, Afu-34, Afu-39, Afu-54, Afu-57, Afu-58, Afu-68, Afu-69) were analysed for five species (beluga, Siberian sturgeon, Russian sturgeon, sterlet and paddlefish) and for their three interspecific hybrids: Siberian sturgeon x Russian sturgeon, beluga x sterlet (called bester) and for beluga x bester. Certain alleles at five loci: Afu-22, Afu-39, Afu-54, Afu-57 and Afu-68 were diagnostic for the examined sturgeon species and their interspecific hybrids.
Źródło:
Environmental Biotechnology; 2006, 2, 1; 11-19
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Gynogenesis in northern pike: UV-inactivation of spermatozoa and the heat shock inducing meiotic diploidization
Autorzy:
Luczynski, M. J.
Dabrowski, K.
Kucharczyk, D.
Glogowski, J.
Luczynski, M.
Szczerbowski, A.
Babiak, I.
Powiązania:
https://bibliotekanauki.pl/articles/363226.pdf
Data publikacji:
2007
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
Esox lucius
gynogeneza
szok termiczny
szczupak pospolity
kontrola płci
UV
gynogenesis
heat shock
northern pike
sex control
Opis:
Gynogenetic northern pike (Esox lucius L.) were produced using UV irradiated sperm and heat shock applied to inseminated eggs shortly after gamete activation. Milt was diluted in immobilizing solution (1:9) and UV irradiated (6.4 W*m-2) for 2-20 min, with dosage in the range of 768-7680 J*m-2. Genetic inactivation of spermatozoa was most efficient when milt was irradiated for 8 min (3072 J*m-2). Insemination of eggs with irradiated milt yielded 100% haploid larvae with hatching rate at 72.1۪.8% (mean±SD), expressed as a percentage of inseminated eggs. Haploid embryo developed and most of them hatched (showing "haploid syndrome") but all haploid larvae died within 48 hours after hatching. After insemination with irradiated sperm the eggs were exposed to a thermal shock of 34°C or 34.5°C, lasting 3 or 5 min, applied 11-16 min after gamete activation. The efficiency of heat shock and survival in experimental groups significantly depended on the source (individual female effect) and quality of eggs. The time of application and duration of the heat shock also affected the survival of embryo and percentage of gynogenetic larvae. Gynogenetic larvae were obtained in all experimental groups with hatching rate as high as 24.2ۭ.6%.
Źródło:
Environmental Biotechnology; 2007, 3, 2; 39-43
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of ancient mitochondrial DNA of the Baltic Sea sturgeon (Acipenser sp.)
Autorzy:
Fopp-Bayat, D.
Ciesielski, S.
Luczyński, M.
Powiązania:
https://bibliotekanauki.pl/articles/363174.pdf
Data publikacji:
2005
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
jesiotr
historyczny mtDNA
filogeneza molekularna
gen cytochromu b
Morze Bałtyckie
Acipenser baeri
Acipenser oxyrinchus
Acipenser sturio
ancient mtDNA
cytochrome b gene
molecular phylogeny
Opis:
Genetic relatedness between Baltic Sea sturgeon (Acipenser sturio L.) specimens caught in different geographic areas is not clear. According to previous studies, fish captured in different locations within the historic area of A. sturio habitation are genetically different to each other. We have examined a fragment (191 base pairs) of mitochondrial cytochrome b gene of four specimens of A. sturio found in Poland: three fish were preserved in museums of natural history and the bone of one fish was from an archaeological site. DNA sequences of the three museum samples were identical, whereas the DNA sequence of the archaeological sample differed in the 918 position of the cytochrome b gene. All the analyzed DNA fragments were similar to those of Acipenser baeri and genetically distant to Acipenser sturio and A. oxyrinchus.
Źródło:
Environmental Biotechnology; 2005, 1, 1; 29-33
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessment of mercury in muscles, liver and gills of marine and freshwater fish
Autorzy:
Luczynska, J.
Luczynski, M.J.
Paszczyk, B.
Powiązania:
https://bibliotekanauki.pl/articles/1189779.pdf
Data publikacji:
2016
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
Tematy:
fish
total mercury
mercury
muscle
liver
gill
marine fish
freshwater fish
body weight
total length
biotic factor
condition factor
Opis:
In this study, the total mercury concentration was determined in the muscles, liver and gills of six fish species (rainbow trout Oncorhynchus mykiss Walb., carp Cyprinus carpio L., bream Abramis brama L., perch Perca fluviatilis L., ide Leuciscus idus L. and flounder Platichthys flesus L.). The fish were acquired from October to November 2012. Mercury was analyzed by atomic absorption spectrometry using thermal decomposition, compounds of mercury and amalgamation. The mercury content in fish organs reached 0.006-0.168 (in mg kgˉ¹ wet weight) in muscles, 0.001-0.027 in gills and 0.003-0.045 in the liver. The muscles of perch and ide had significantly more Hg compared to the other fish’s muscles (P≤0.05). The liver and gills of perch, ide and flounder contained more Hg than the same tissues of the other fish (P≤0.05). Generally, the highest Hg content was determined in muscles (except rainbow trout) (P≤0.05), whereas the lowest Hg content was found in gills (except perch) (P≤0.05). The content of Hg in gills of perch did not differ from the one in the liver (P>0.05). There was a positive correlation between the weight or length of a fish and the Hg concentration in its tissues, except for the length and Hg in the gills of carp. However, a statistically significant positive correlation between the body weight and the Hg levels in fish appeared only in the case of the organs of ide (P≤0.004) and muscles of carp (P≤0.038). The correlation between the factor condition and the content of Hg, albeit positive (0.106 < r < 0.811) except for the organs of flounder, was not statistically significant (P>0.05).
Źródło:
Journal of Elementology; 2016, 21, 1; 113-129
1644-2296
Pojawia się w:
Journal of Elementology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Preliminary evaluation of mutagenic activity of two amino derivatives of cyclopenta[c]phenanthrene
Autorzy:
Łuczyński, M. K.
Góra, M.
Mielżyńska, D.
Brzuzan, P.
Tejs, S.
Pirogowicz, P.
Luczynski, M.
Powiązania:
https://bibliotekanauki.pl/articles/363278.pdf
Data publikacji:
2007
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
test Amesa
fenantren
mutacje DNA
WWA
Ames test
phenanthrene
DNA mutations
PAHs
Opis:
The mutagenicity of two, newly synthesized amino-derivatives of cyclopenta[c]phenanthrene (CP[c]Ph) was investigated in the Ames test using TA98 and TA100 histidine dependent Salmonella typhimurium strains. Neither of the examined compounds showed mutagenic activity without metabolic activation. The incorporation of an activation system caused high mutagenicity of CP[c]Ph derivatives in both S. typhimurium strains. The results are compared with the previous data on the mutagenic activity of unsubstituted CP[c]Ph and possible mechanisms of activation are discussed.
Źródło:
Environmental Biotechnology; 2007, 3, 1; 15-19
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genotoxicity of cyclopentha[c]phenanthrene and its two derivatives based on an in vitro micronucleus test
Autorzy:
Rudnicka, K.
Łuczyński, M. K.
Góra, M.
Powiązania:
https://bibliotekanauki.pl/articles/363298.pdf
Data publikacji:
2012
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
derivatives
genotoxicity
in vitro micronucleus test
PAHs
V79
pochodne
genotoksyczność
test mikrojądrowy in vitro
Opis:
Environmental pollution causes a variety of health problems, including cancer. Many known pollutants have carcinogenic properties and polycyclic aromatic hydrocarbons (PAH) belong to this group. In this study, an in vitro culture of V79 cells of the Chinese hamster was subjected to three tested PAHs: 5-amino-2,3-dihydro-1H-cyclopenta-[c]phenanthrene (ACP[c]Ph), 5-amino-9-methoxy-2,3-dihydro-1H-cyclopenta[c]phenanthrene (AMCP[c]Ph) and cyclopenta[c]phenanthrene (CP[c]Ph). The in vitro micronucleus (MN) assay was applied in order to evaluate the genotoxic properties of the studied compounds. The highest genotoxic effect was observed for AMCP[c]Ph in a concentration of 0.02μg·ml-1. The genotoxic effect of the other two compounds was slightly lower.
Źródło:
Environmental Biotechnology; 2012, 8, 2; 63-66
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessment of mercury in muscles, liver and gills of marine and freshwater fish
Autorzy:
Luczynska, J.
Luczynski, M.J.
Paszczyk, B.
Powiązania:
https://bibliotekanauki.pl/articles/13699.pdf
Data publikacji:
2016
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
Tematy:
fish
total mercury
mercury
muscle
liver
gill
marine fish
freshwater fish
body weight
total length
biotic factor
condition factor
Opis:
In this study, the total mercury concentration was determined in the muscles, liver and gills of six fish species (rainbow trout Oncorhynchus mykiss Walb., carp Cyprinus carpio L., bream Abramis brama L., perch Perca fluviatilis L., ide Leuciscus idus L. and flounder Platichthys flesus L.). The fish were acquired from October to November 2012. Mercury was analyzed by atomic absorption spectrometry using thermal decomposition, compounds of mercury and amalgamation. The mercury content in fish organs reached 0.006-0.168 (in mg kgˉ¹ wet weight) in muscles, 0.001-0.027 in gills and 0.003-0.045 in the liver. The muscles of perch and ide had significantly more Hg compared to the other fish’s muscles (P≤0.05). The liver and gills of perch, ide and flounder contained more Hg than the same tissues of the other fish (P≤0.05). Generally, the highest Hg content was determined in muscles (except rainbow trout) (P≤0.05), whereas the lowest Hg content was found in gills (except perch) (P≤0.05). The content of Hg in gills of perch did not differ from the one in the liver (P>0.05). There was a positive correlation between the weight or length of a fish and the Hg concentration in its tissues, except for the length and Hg in the gills of carp. However, a statistically significant positive correlation between the body weight and the Hg levels in fish appeared only in the case of the organs of ide (P≤0.004) and muscles of carp (P≤0.038). The correlation between the factor condition and the content of Hg, albeit positive (0.106 < r < 0.811) except for the organs of flounder, was not statistically significant (P>0.05).
Źródło:
Journal of Elementology; 2016, 21, 1
1644-2296
Pojawia się w:
Journal of Elementology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
East-Siberian coregonid fishes: their occurrence, evolution and present status
Autorzy:
Yakhnenko, V. M.
Mamontov, A. M.
Luczynski, M.
Powiązania:
https://bibliotekanauki.pl/articles/363220.pdf
Data publikacji:
2008
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
system wodny
zlodowacenie
aktywność tektoniczna
jezioro
pochodzenie
coregonidy
specjacja
sympatryczność
allopatry
coregonids
glaciation
origin of lakes
speciation
sympatry
tectonic activity
water systems
Opis:
Coregonid fishes (coregonids) (Salmonidae, Coregoninae) of Siberia show immense morphological and ecological variability, what is reflected in their complex interspecific and intraspecific structure: i.e. occurrence of numerous species, forms and endemic species. Studies of the coregonids of Siberia is crucially important for understanding of their origin, taxonomy and evolution. We have analysed electrophoretically some 13 enzyme systems from over 20 samples of East-Siberian coregonids. This paper was intended as an introduction to a number of primary papers that are planned that will describe the biological and genetic characteristics of studied populations.
Źródło:
Environmental Biotechnology; 2008, 4, 2; 41-53
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Polymorphism of microsatellite loci - a tool in studying biodiversity of paddlefish aquaculture broodstock
Autorzy:
Kaczmarczyk, D.
Kohlmann, K.
Kersten, P.
Luczynski, M.
Powiązania:
https://bibliotekanauki.pl/articles/363088.pdf
Data publikacji:
2007
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
izolacja DNA
DNA mikrosatelitarne
amplifikacja PCR
DNA extraction
microsatellite DNA
PCR amplification
Opis:
American paddlefish (Polyodon spathula) is a new species in Polish aquaculture, its broodstocks are few and small, and it is possible that all mature fish originated from only a few spawners. Studies on polymorphism of highly variable microsatellite DNA allow revealing genetic characteristics of individual spawners as well as estimation of genetic variation within and divergence between broodstocks. This paper describes optimised protocols for isolation of DNA from fin tissues, amplification of nine microsatellite loci using PCR technique, and for fish genotyping using automatic capillary DNA sequencer. Our technique was tested towards the fin samples taken from all paddlefish reared in Poland and approaching their sexual maturity; the study included also samples taken from 47 fish of the Ukrainian breeding center (Gorny Tykich).
Źródło:
Environmental Biotechnology; 2007, 3, 2; 44-48
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Relative quantification of CYP1A gene expression in whitefish (Coregonus lavaretus) exposed to benzo[a]pyrene
Autorzy:
Brzuzan, P.
Jurczyk, Ł.
Łuczyński, M. K.
Góra, M.
Powiązania:
https://bibliotekanauki.pl/articles/363188.pdf
Data publikacji:
2005
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
ekspresja genu CYP1A
sieja
benzo(a)piren
real-time PCR
benzo(a)pyrene
Coregonus lavaretus
CYP1A gene expression
RealTime PCR
relative quantification
Opis:
The expression of CYP1A (cytochrome P4501A) can be induced by a number of aromatic compounds in teleost fishes. We developed a real-time PCR assay for measuring relative quantities (RQ) of CYP1A mRNA in whitefish (Coregonus lavaretus). To test for the usefulness of the assay we performed a treatment study, using benzo[a]pyrene (B[a]P) a model CYP1A inducer. Primers for the CYP1A gene were adapted from the literature, whereas those for [beta]-actin (endogenous control) were designed from a region that was found to be conserved among salmonid [beta]-actin genes. A group of hatchery raised whitefish, with an average body mass of 15 g and total length of 12 cm were given an intraperitoneal injection (10 mg/kg) of B[a]P in corn oil (2 mg B[a]P/ml corn oil) or corn oil alone (Control). After 48 h, whitefish liver, head kidney and brains were collected for mRNA isolation and analysis. In all three tissues sampled, CYP1A mRNA was affected by treatment with B[a]P. Head kidney tissue showed the greatest induction potential (RQ=11.00) from base levels (RQ=1.00), followed by liver (RQ=9.45), and brain (RQ=3.76). These results demonstrated that CYP1A was highly inducible by B[a]P in whitefish head kidney and liver, and to some extent, in brain tissue. The approach presented here has the advantage of providing rapid and accurate measures of CYP1A induction in various tissues of fish responding to PAH contaminant exposure.
Źródło:
Environmental Biotechnology; 2005, 1, 1; 11-15
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of by-products and waste in the synthesis of nanosilver particles
Autorzy:
Trepanowska, B.
Łuczyński, M. K.
Kulesza, S.
Adamczak, M.
Powiązania:
https://bibliotekanauki.pl/articles/363244.pdf
Data publikacji:
2015
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
nanobiotechnology
nanoparticles
nanosilver
plant extracts
raspberry
strawberry
nanotechnologia
nanocząstki
nanosrebro
ekstrakt roślinny
malina
truskawka
Opis:
Extracts from strawberry and raspberry leaves, carrot pomace, and spent grains, were tested as bioreducing agents for the synthesis of nanosilver particles (AgNP). Based on UV vis spectra, the leaf extracts produced the most AgNP, carrot pomace was less effective, and spent grains did not produce AgNP. The dynamic light scattering method revealed that AgNP ranged from 1 to 92nm in size, and that over 80% of the particles were about 10nm. Energy dispersive X ray spectroscopy showed that elements that typically stabilize nanoparticles were present. The well diffusion method (nutrient agar medium) indicated that AgNP synthesized with raspberry leaf extract exerted strong bacteriostatic and bactericidal activity against Gram negative bacteria and weaker activity against Gram positive bacteria. Although further analysis is needed to determine the mechanism of their synthesis, the results of this study show that plant extract based synthesis can produce nanoparticles with controlled size and morphology.
Źródło:
Environmental Biotechnology; 2015, 11, 1; 14-19
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
CYP1A gene expression in adipose fin of rainbow trout (Oncorhynchus mykiss Walbaum) exposed to benzo[a]pyrene
Autorzy:
Brzuzan, P.
Woźny, M.
Łuczyński, M. K.
Góra, M.
Powiązania:
https://bibliotekanauki.pl/articles/363276.pdf
Data publikacji:
2007
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
płetwa tłuszczowa
benzo(a)piren
CYP1A mRNA
skrzela
pstrąg tęczowy
real-time PCR
adipose fin
benzo(a)pyrene
gill
rainbow trout
Real-Time PCR
Opis:
Proximate to the environment, adipose fin of fish may be considered as a lipid storing tissue, and thus can be a target for either waterborne or dietary polycyclic aromatic compounds (PACs). We determined the effects of benzo[a]pyrene (B[a]P), a model PAC member, on CYP1A gene expression in adipose fin and compared that with the effects in gill of juvenile rainbow trout (Oncorhynchus mykiss Walbaum) using the quantitative reverse transcription polymerase chain reaction (Q-RT-PCR). The results of the study demonstrated that constitutive CYP1A mRNA was present in adipose fin of rainbow trout, but the transcripts were far less abundant than those in gill tissue. We confirmed high CYP1A gene induction potential of the gills in rainbow trout injected with benzo[a]pyrene, but also showed moderately and transiently induced CYP1A mRNA in adipose fin. The modest and transitory gene expression may preclude rainbow trout adipose fin CYP1A mRNA levels from using it as an indicator of sustained exposure of fish to the polycyclic aromatic compounds.
Źródło:
Environmental Biotechnology; 2007, 3, 1; 20-24
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Oxidative metabolism, mutagenic and carcinogenic properties of some polycyclic aromatic hydrocarbons
Autorzy:
Łuczyński, M. K.
Góra, M.
Brzuzan, P.
Wilamowski, J.
Kozik, B.
Powiązania:
https://bibliotekanauki.pl/articles/363172.pdf
Data publikacji:
2005
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
metabolizm tlenowy
właściwości mutagenne
właściwości rakotwórcze
wielopierścieniowe węglowodory aromatyczne
oxidative metabolism
mutagenic properties
carcinogenic properties
polycyclic aromatic hydrocarbons
Opis:
This attempt to synthesize the present state of knowledge on the correlation between the structure of chosen PAHs and their mutagenic or carcinogenic activity shows that the dependencies cannot be treated univocally. Extreme complexity of the subject, typical for investigations on the boundary between chemistry and biology, inclines one to search for new ways of synthesis of new compounds, which would be of interest for biologists studying the problem of chemical carcinogenesis. On the other hand, there is a strong need for elaborating a standardized test that would explicitly define the carcinogenic properties of a specific compound. The use of a variety of methods for the determination of carcinogenic potency of chemical compounds combined with simultaneous differentiation of structure of examined compounds, sometimes leads to contradictory conclusions. Correlation between mutagenic and carcinogenic activity of some compounds is also sometimes questionable. Modern molecular biology should allow for elaborating a method which would give a satisfactory and reliable answer to the fundamental question: is a specific compound carcinogenous for a human organism? And if so - to what extent?
Źródło:
Environmental Biotechnology; 2005, 1, 1; 16-28
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
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