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Wyświetlanie 1-15 z 18
Tytuł:
In vitro co-stimulation of anti-tumor activity by soluble B7 molecules
Autorzy:
He, Wei
Hu, Zhong-Bo
Liu, Fang
Feng, Xian-Qi
Zou, Ping
Powiązania:
https://bibliotekanauki.pl/articles/1041178.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
immunotherapy
tumor escape
gene fusion
recombinant fusion proteins
Opis:
In order to investigate the anti-tumor activity of a soluble B7-1/immunoglobulin G fusion protein and explore an effective method to eliminate immune escape of tumor cells, a recombinant vector encoding this fusion protein was constructed and constitutively expressed in Chinese hamster ovary cells. After purification with protein G affinity chromatography, the soluble fusion protein was tested for bioactivity. Results showed that the fusion protein could significantly increase the density of B7-1 molecules on WEHI-3 cells, a mouse leukemia cell line. Through allogeneic mixed lymphocyte tumor cultures, it was demonstrated that, with the presence of the first signal, it could also significantly enhance T cell activation and killing activity against WEHI-3 cells and interleukin-2 secretion by activated mouse T lymphocytes. The conclusion can be drawn that the soluble B7-IgG fusion protein has a potent capacity to generate or enhance anti-tumor immune response in vitro, and its clinical value deserves further investigation.
Źródło:
Acta Biochimica Polonica; 2006, 53, 4; 807-813
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Upregulation of GRP78 and caspase-12 in diastolic failing heart
Autorzy:
Sun, Yingying
Liu, Guanghui
Song, Tao
Liu, Fang
Kang, Weiqiang
Zhang, Yun
Ge, Zhiming
Powiązania:
https://bibliotekanauki.pl/articles/1040708.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
caspase-12
diastolic heart failure
GRP78; hypertension
apoptosis
Opis:
Background: The endoplasmic reticulum (ER) fulfills multiple cellular functions. Various stimuli can potentially cause ER stress (ERS). ERS is one of the intrinsic apoptosis pathways and apoptosis plays a critical role in hypertension. Glucose regulated protein 78 (GRP78) has been widely used as a marker for ERS and caspase-12 mediated apoptosis was a specific apoptotic pathway of ER. The expression of GRP78 and caspase-12 remains poorly understood in the diastolic heart failure resulting from hypertension. Methods: We used spontaneously hypertensive rats (SHRs) to establish a model of diastolic heart failure, and performed immunohistochemistry, western blot, and real-time PCR to analyze GRP78 and caspase-12. Results: We found that GRP78 and caspase-12 had enhanced expression at protein and mRNA levels. Conclusions: These results suggest that GRP78 and caspase-12 were upregulated in cardiomyocytes and ERS can contribute to cardiac myocyte apoptosis in the diastolic heart failure resulting from hypertension.
Źródło:
Acta Biochimica Polonica; 2008, 55, 3; 511-516
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Investigations on a novel collector for anionic reverse flotation separation of quartz from iron ores
Autorzy:
Fang, Ji
Ge, Yingyong
Liu, Shunbing
Yu, Jun
Liu, Cheng
Powiązania:
https://bibliotekanauki.pl/articles/1448585.pdf
Data publikacji:
2021
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
reverse flotation
iron ore
quartz
collector
fatty acid
Opis:
It is necessary to exploit an easy and effective way to obtain flotation collector. In this paper, oleic acid was modified by oxidation reaction to obtain an excellent collector. The flotation performances of oleic acid and its oxidation products were systematically investigated and a novel collector ((E)-8,11-dihydroperoxyoctadec-9-enoic acid, EDEA) was obtained. Single mineral flotation tests results showed that EDEA had strong flotation power towards quartz at pH＞11 and hardly floated hematite and magnetite at pH=8~13. The recovery of quartz could achieve 92.35% with 120 mg/L EDEA under the condition of pH=12 and dosage of $CaCl_2$ 60mg/L while the recovery was 62.44% with 120 mg/L oleic acid. Bench scale flotation tests results showed that EDEA had a preferable effect on separating quartz from iron ore especially at low flotation temperature (288K). The mechanism research revealed that EDEA attracted on quartz surface through electrostatic attraction and the neighboring EDEA molecules could form intermolecular hydrogen bonds which resulted in a closer alignment of EDEA on quartz surface.
Źródło:
Physicochemical Problems of Mineral Processing; 2021, 57, 1; 136-155
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Research on the maritime logistics pricing model of risk-averse retailer-dominated dual-channel supply chain
Autorzy:
Fang, Q.
Tong, Z.
Ren, L.
Liu, A.
Powiązania:
https://bibliotekanauki.pl/articles/258648.pdf
Data publikacji:
2018
Wydawca:
Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
Tematy:
dual channel
risk-averse
retailer-dominated
supply chain
Opis:
Price decision is studied in a risk-averse retailer-dominated dual-channel supply chain, which consisting of one manufacturers and one retailer with both off-line and on-line channels. Firstly, two mean-variance models in centralized and decentralized supply chain are established. Secondly, the optimal solutions under the two decision modes are compared and analyzed. The results shows that the price of dual-channel of retailer decreased with the increase of retailers’ risk- aversion coefficient and the standard deviation of the fluctuation of market demand, while the wholesale price changes is on the contrary; in addition, when the market demand is greater than a certain value, the prices of dual channel are correspondingly higher in decentralized supply chain than in centralized supply chain, and vice versa. In addition, when the retailer’s risk aversion is in a certain interval, the expected utility of the whole supply chain is greater in centralized supply chain than in decentralized decision, and vice versa. Finally, a numerical example is given to verify the above conclusions.
Źródło:
Polish Maritime Research; 2018, S 2; 107-116
1233-2585
Pojawia się w:
Polish Maritime Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Accurate Frequency Estimation Based On Three-Parameter Sine-Fitting With Three FFT Samples
Autorzy:
Liu, X.
Ren, Y.
Chu, C.
Fang, W.
Powiązania:
https://bibliotekanauki.pl/articles/220451.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
frequency estimation
CRLB
three-parameter sine-fitting
RMSE
golden section
Opis:
This paper presents a simple DFT-based golden section searching algorithm (DGSSA) for the single tone frequency estimation. Because of truncation and discreteness in signal samples, Fast Fourier Transform (FFT) and Discrete Fourier Transform (DFT) are inevitable to cause the spectrum leakage and fence effect which lead to a low estimation accuracy. This method can improve the estimation accuracy under conditions of a low signal-to-noise ratio (SNR) and a low resolution. This method firstly uses three FFT samples to determine the frequency searching scope, then – besides the frequency – the estimated values of amplitude, phase and dc component are obtained by minimizing the least square (LS) fitting error of three-parameter sine fitting. By setting reasonable stop conditions or the number of iterations, the accurate frequency estimation can be realized. The accuracy of this method, when applied to observed single-tone sinusoid samples corrupted by white Gaussian noise, is investigated by different methods with respect to the unbiased Cramer-Rao Low Bound (CRLB). The simulation results show that the root mean square error (RMSE) of the frequency estimation curve is consistent with the tendency of CRLB as SNR increases, even in the case of a small number of samples. The average RMSE of the frequency estimation is less than 1.5 times the CRLB with SNR = 20 dB and N = 512.
Źródło:
Metrology and Measurement Systems; 2015, 22, 3; 403-416
0860-8229
Pojawia się w:
Metrology and Measurement Systems
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Improved virtual space vector modulation for neutral point voltage oscillation and common-mode voltage reduction in neutral point clamped three-level inverter
Autorzy:
Fang, Junlong
Wang, Guangya
Li, Ran
Liu, Siyuan
Wang, Shuyu
Powiązania:
https://bibliotekanauki.pl/articles/1841339.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
three-level inverter
vector conversion
virtual space vector modulation
falownik trójpoziomowy
konwersja wektorowa
modulacja wektora przestrzeni wirtualnej
Opis:
The neutral point clamped (NPC) three-level inverter is widely used in high-voltage and high-power applications. However, neutral point voltage oscillation (NPVO) and common-mode voltage (CMV) problems exist in the NPC three-level inverter. In this paper, an improved virtual space vector modulation (VSVM) is proposed based on the reconstruction of a virtual small vector and a virtual medium vector. Compared with the traditional VSVM, an improved VSVM can effectively reduce the CMV. On this basis, a vector conversion method is proposed to further reduce the NPVO in the whole range. Simulation results verify the effectiveness and superiority of the improved VSVM.
Źródło:
Archives of Electrical Engineering; 2021, 70, 1; 203-218
1427-4221
2300-2506
Pojawia się w:
Archives of Electrical Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Balancing reliability and maintenance cost rate of multi-state components with fault interval omission
Równoważenie wskaźników niezawodności i kosztów utrzymania elementów wielostanowych z pominięciem przedziału wystąpienia uszkodzenia
Autorzy:
Dong, Wenjie
Liu, Sifeng
Yang, Xiaoyu
Wang, Huan
Fang, Zhigeng
Powiązania:
https://bibliotekanauki.pl/articles/301323.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Polskie Naukowo-Techniczne Towarzystwo Eksploatacyjne PAN
Tematy:
multi-state systems (MSSs)
fault effect omission
stochastic process
repairable model
maintenance cost rate
systemy wielostanowe (MSS)
pominięcie wpływu uszkodzenia
proces stochastyczny
model naprawialny
polityka utrzymania ruchu
Opis:
For the repairable multi-state component, reliability indexes are analyzed based on a homogenous Continuous Time Markov Chain (CTMC). If the component can work well when its repair time is sufficiently short, a threshold value for maintenance is introduced. When the fault interval is less than threshold time, the fault effect is considered neglected. In this paper, comparisons of availability show differences of the new model and the original model with or without fault interval omission. In addition, balancing the maintenance cost and lifetime of multi-state components is an important issue when threshold values are considered. Both constants and non-negative random variables are modeled respectively. Finally, numerical examples are presented to illustrate the results obtained in this paper.
W przypadku naprawialnych elementów wielostanowych, wskaźniki niezawodności analizuje się w oparciu o łańcuch Markowa z czasem ciągłym. Jeśli element może działać prawidłowo, mimo uszkodzenia, dzięki wystarczająco krótkiemu czasowi naprawy, wprowadza się próg czasowy dla konserwacji. Gdy przedział czasu, w którym następuje uszkodzenie jest krótszy niż próg czasowy dla działań konserwacyjnych, wpływ uszkodzenia uważa się za nieistotny. Przeprowadzone w niniejszym artykule porównania gotowości wykazały różnice między nowym modelem a modelem oryginalnym z pominięciem lub bez pominięcia przedziału wystąpienia uszkodzenia. Ponadto, przy rozważaniu wartości progowych, ważną kwestią jest równoważenie kosztów utrzymania i żywotności elementów wielostanowych. W pracy próg wystąpienia uszkodzenia zamodelowano, odpowiednio, zarówno jako wartość stałą jak i nieujemną zmienną losową. Na koniec przedstawiono przykłady ilustrujące wyniki przedstawionych badań.
Źródło:
Eksploatacja i Niezawodność; 2019, 21, 1; 37-45
1507-2711
Pojawia się w:
Eksploatacja i Niezawodność
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An approach to suppress low-frequency oscillation in the hybrid multi-Infeed HVDC of mixed H2/H∞ robust-based control theory
Autorzy:
Li, Congshan
Liu, Yan
Li, Yikai
He, Ping
Fang, Yan
Sheng, Tingyu
Powiązania:
https://bibliotekanauki.pl/articles/2042784.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
additional damping controller
CSP
HMIDC
LFO
mixed 2/∞ control
TLS-ESPRIT
Opis:
A hybrid multi-infeed HVDC (HMIDC) system is composed of line-commutated converter-based high-voltage direct current (LCC-HVDC) and voltage-source converterbased high-voltage direct current (VSC-HVDC), whose receiving ends have electrical coupling. To solve the problem of low-frequency oscillation (LFO) caused by insufficient damping in the HMIDC system, according to the multi-objective mixed 2/∞ output feedback control theory with regional pole assignment, an additional robust damping controller is designed in this paper, which not only has good robustness, but also has strong adaptability to the change of system operation mode. In the paper, the related oscillation modes and transfer function of the controlled plant are obtained, which are identified by the total least squares estimation of signal parameters via rotary invariance technology (TLS-ESPRIT). In addition, the control-sensitive point (CSP) for suppressing LFO based on the small disturbance test method is determined, which is suitable for determining the installation position of the controller. The results show that the control sensitivity factor of VSC-HVDC is greater than that of LCC-HVDC so that adding an additional damping controller to VSC-HVDC is better than LCC-HVDC in suppressing the LFO of HMIDC. Finally, a hybrid double infeed DC transmission system with three receiving terminals is built on PSCAD/EMTDC where the time-domain simulations are performed to verify the correctness of the CSP selection and the effectiveness of the controller.
Źródło:
Archives of Electrical Engineering; 2022, 71, 1; 109-124
1427-4221
2300-2506
Pojawia się w:
Archives of Electrical Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of polyacrylamide on the process of removing impurities in the rare earth leachate
Autorzy:
Wu, Xiaoyan
Zhou, Fang
Liu, Chufan
Feng, Jian
Zhang, Zhenyue
Chi, Ruan
Powiązania:
https://bibliotekanauki.pl/articles/1448504.pdf
Data publikacji:
2021
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
polyacrylamide
rare earth leachate
impurity removal
flocs
Opis:
The impurity removal process of the rare earth (RE) leachate is usually accompanied by the formation of flocs, and a certain amount of polyacrylamide flocculant needs to be added in this process. However, few studies have investigated the effect of the flocculant on the impurity removal process. Therefore, this paper compares the influence on the process of removing impurities with or without the adding of polyacrylamide (PAM). The results showed that the addition of PAM had little effect on the removal rate of impurities, but it was conducive to the recovery of RE. When ammonium bicarbonate was firstly adopted to regulate the solution pH to 5.0, and then sodium sulfide was used to adjust the pH to 5.2, and 0.5 mL/100 mL of PAM was added to the leachate, 96% $Al^{3+}$ and 98% $Zn{2+}$ were removed from the leachate, while 95% RE was maintained. In addition, PAM could accelerate the settlement of flocs and then shorten the production cycle. The increase in RE recovery rate may be due to the addition of PAM to shorten the settling time of the flocs, thereby reducing the RE ions adsorbed during the flocculation process and increasing the recovery rate of the RE.
Źródło:
Physicochemical Problems of Mineral Processing; 2021, 57, 1; 182-191
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Direct reuse of rare earth oxalate precipitation mother liquor for rare earth leaching
Autorzy:
Wu, Xiaoyan
Zhou, Fang
Feng, Jian
Liu, Xueme
Zhang, Zhenyue
Chi, Ruan
Powiązania:
https://bibliotekanauki.pl/articles/109869.pdf
Data publikacji:
2019
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
weathered crust elution-deposited rare earth ore
oxalic acid
precipitation
mother liquor
Opis:
In the recovery process of rare earth (RE) from the weathered crust elution-deposited rare earth ore, ammonium sulfate is used as the leaching liquor to leach RE, and then the leachate containing RE3+ can be precipitated by oxalic acid and the RE oxalate precipitation mother liquor is reused for RE leaching process after removing the residual oxalic acid by precipitation with calcium hydroxide. However, the reuse process of precipitation mother liquor cannot proceed in the strong acid and alkali restricted areas and the discharge of mother liquor which contains a large amount of ammonium salt will cause ammonia-nitrogen waste and pollution. In order to realize the reuse of the precipitation mother liquor in this area, the direct reuse of RE oxalate precipitation mother liquor for RE leaching was investigated in this study. The RE oxalate precipitation process and the RE leaching process with oxalic acid were studied. The results showed that the residual oxalic acid concentration in the mother liquor can be controlled lower than 0.8 g/dm3 at pH 2-3 when the RE concentration in the leachate was 0.1- 1.5 g/dm3 and the RE precipitation rate reached to 94%. In addition, RE leaching efficiency was up to 90% while the oxalic acid concentration in the prepared mother liquor was 0.2-0.8 g/dm3, pH 2-3. Therefore, the precipitation mother liquor with oxalic acid concentration less than 0.8 g/dm3 could be directly reused for RE leaching. However, considering the different performance of RE ores, the recommended oxalic acid concentration in the direct used precipitation mother liquor was lower than 0.6 g/dm3.
Źródło:
Physicochemical Problems of Mineral Processing; 2019, 55, 3; 760-769
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of Ultraviolet Absorber on Photo-Degradation of Epoxy Coating Studied by Slow Positron Beam
Autorzy:
Wang, Zheng
Liu, Fuwei
Li, Jingjing
He, Chunqing
Peng, Xiangyang
Huang, Zhen
Fang, Pengfei
Powiązania:
https://bibliotekanauki.pl/articles/1033239.pdf
Data publikacji:
2017-11
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
epoxy coating
photo-degradation
ultraviolet absorber
slow positron beam
microstructure
Opis:
The photo-degradation progress of epoxy coating and the effect of ultraviolet absorber under UV-B irradiation have been investigated in slow positron beam and by the Fourier transform infrared spectrometer. After 120 h of irradiation, the value of S parameter in sample bulk is reduced while compared with the virgin sample. The result is mostly due to post-cure process happening in this initial irradiation stage. As the irradiation time increases to 360 h, the S parameter decreases sharply. This is due to the growth of carbonyl group and the generation of free radical. After 528 h or longer time of irradiation, a very low S value was obtained near sample surface, indicating the formation of a dead surface layer. Positron results also reveal that the addition of ultraviolet absorber suppresses the development of the dead layer after long-term UV-B irradiation. Ultraviolet absorber has a suppressing effect on generation of polar groups towards sample bulk. The addition of ultraviolet absorber is a key factor that affects the photo-degradation of epoxy coating.
Źródło:
Acta Physica Polonica A; 2017, 132, 5; 1523-1526
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Photo-oxidative Aging Behaviors of Acrylic Coatings during UV Irradiation Investigated by Positron Annihilation Spectroscopy
Autorzy:
Zhao, Xipo
Cai, Xiaolin
Liu, Chang
Chen, Ting
He, Chunqing
Fang, Pengfei
Peng, Shaoxian
Powiązania:
https://bibliotekanauki.pl/articles/1033253.pdf
Data publikacji:
2017-11
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
Acrylic coating
UV irradiation
positron annihilation spectroscopy
Opis:
Slow positron annihilation spectroscopy, contact angle and scanning electron microscope (SEM) were employed to investigate the surface polarity and morphologies as functions of aging time in acrylic coatings during UV-a irradiation. The S parameter plots of each exposed sample are lower than that of the virgin sample in the whole range of E. The S parameter plots become lower as the exposing longer time, indicated that the irradiation generated more polar groups and increased the oxidation layer depth. Combined with surface morphology and the surface energy of acrylic coating, the variation of contact angle, and surface energy, polar component and dispersive component is related with surface polarity and morphologies.
Źródło:
Acta Physica Polonica A; 2017, 132, 5; 1527-1530
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Morphological differentiation and phylogenetic homogeneity in Usnea aurantiaco-atra reveal the complexity of lichen symbiosis
Autorzy:
Cao, Shunan
Zheng, Hongyuan
Cao, Yunshu
Liu, Chuanpeng
Zhu, Lingxiang
Peng, Fang
Zhou, Qiming
Powiązania:
https://bibliotekanauki.pl/articles/2042115.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Antarctic
lichens
symbiont
ITS
molecular phylogeny
morphology
anatomy
Źródło:
Polish Polar Research; 2018, 39, 2; 313-328
0138-0338
2081-8262
Pojawia się w:
Polish Polar Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Adsorption of lanthanum(III) and yttrium(III) on kaolinite: kinetics and adsorption isotherms
Autorzy:
Zhou, Fang
Feng, Jian
Xie, Xiong
Wu, Baihong
Liu, Qi
Wu, Xiaoyan
Chi, Ruan
Powiązania:
https://bibliotekanauki.pl/articles/109951.pdf
Data publikacji:
2019
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
adsorption
kaolinite
rare earth
kinetics
Opis:
Experimental investigations were carried out using kaolinite to adsorb two rare earth ions, lanthanum ion (La3+) and yttrium ion (Y3+), which will provide some useful information and new insights on the mineralization process and fractionation phenomenon of weathered crust elution-deposited rare earth ores. The results showed that the equilibrium adsorption capacity of Y3+ is greater than La3+ under the same experimental conditions. The adsorption of rare earth ions presents strongly temperature dependent indicating an endothermic adsorption process. The pseudo-first-order kinetic model and the pseudo-second-order kinetic model were applied to discuss the adsorption kinetics. It was found that the adsorption rate of rare earth follows the pseudo-second-order kinetic model among the adsorption temperature range. Furthermore, the adsorption process of rare earth ions on kaolinite followed the Langmuir isotherm model confirmed by the correlation of experimental equilibrium data to standard isotherm model, Langmuir and Freundlich isotherms. The activation energies for the adsorption of La3+ and Y3+ on kaolinite are 28.1903 kJ/mol and 25.4190 kJ/mol, respectively. All kaolinite before and after adsorption were characterized by XRD and SEM-EDX to understand the adsorption mechanism. The obtained results suggested that the adsorption of La3+ and Y3+ on kaolinite is an endothermic and chemisorption process.
Źródło:
Physicochemical Problems of Mineral Processing; 2019, 55, 4; 928-939
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A novel tetrameric lectin from Lycoris aurea with four mannose binding sites per monomer
Autorzy:
Liu, Jiwei
Xu, Xiaochao
Liu, Jinzhi
Balzarini, Jan
Luo, Yongtin
Kong, Yang
Li, Jian
Chen, Fang
Van Damme, Els
Bao, Jinku
Powiązania:
https://bibliotekanauki.pl/articles/1041131.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
sequence alignment
mannose-binding lectin
Lycoris aurea agglutinin
molecular modeling and docking
Opis:
The mannose-binding agglutinin from bulbs of Lycoris aurea (LAA) agglutinates rabbit but not human erythrocytes. The molecular mass of the monomer in SDS/PAGE is 12 kDa while the apparent molecular mass in gel filtration is 48 kDa, indicating that LAA is a homotetramer. The full-length cDNA of LAA contains 683 bp with an open reading frame encoding a protomer of 162 amino-acid residues. Hydrophobic Cluster Analysis and molecular modeling of the 109-residue mature polypeptide suggested a similar secondary and tertiary structure to those of Narcissus pseudonarcissus agglutinin (NPA). Molecular docking revealed that, besides the three mannose-binding sites common among Amaryllidaceae lectins, LAA also contains a fourth unique mannose-binding site formed by a tryptophan cluster. The existence of four mannose-binding sites in each monomer of LAA is very unusual and has only been reported for NPA earlier.
Źródło:
Acta Biochimica Polonica; 2007, 54, 1; 159-166
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
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