Wszystkie pola: Han, Y. - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Triebel-Lizorkin spaces on spaces of homogeneous type
Autorzy:: Han, Y.-S
Powiązania:: https://bibliotekanauki.pl/articles/1290774.pdf
Data publikacji:: 1994
Wydawca:: Polska Akademia Nauk. Instytut Matematyczny PAN
Tematy:: spaces of homogeneous type
$H^p$ and Triebel-Lizorkin spaces
Littlewood-Paley S-function
atomic decomposition
Opis:: In [HS] the Besov and Triebel-Lizorkin spaces on spaces of homogeneous type were introduced. In this paper, the Triebel-Lizorkin spaces on spaces of homogeneous type are generalized to the case where $p_0 < p ≤ 1 ≤ q < ∞$, and a new atomic decomposition for these spaces is obtained. As a consequence, we give the Littlewood-Paley characterization of Hardy spaces on spaces of homogeneous type which were introduced by the maximal function characterization in [MS2].
Źródło:: Studia Mathematica; 1994, 108, 3; 247-273
0039-3223
Pojawia się w:: Studia Mathematica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Interaction between mineral particles during ascharite flotation process and direct force measurement using AFM
Autorzy:: Li, Z.
Han, Y.
Li, Y.
Gao, P.
Powiązania:: https://bibliotekanauki.pl/articles/110122.pdf
Data publikacji:: 2017
Wydawca:: Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:: ascharite
serpentine
quartz
interaction
AFM
DLVO
Opis:: Interaction between mineral particles during ascharite flotation was investigated by flotation, zeta potential, Derjaguin-Landau-Verwey-Overbeek (DLVO) theory, atomic force microscope (AFM) and SEM-EDS tests. Flotation results showed that the ascharite recovery decreased significantly in the presence of serpentine. In order to improve the ascharite recovery, negative charged quartz was used to limit the detrimental effect of serpentine on ascharite flotation in this research. The effect of quartz on improving ascharite recovery was attributed to the particle surface interactions. The DLVO theory was applied to study the particles interaction in the aqueous solution. The fitting curves showed that serpentine could attach to the surface of quartz easily, and quartz would be well dispersed with ascharite. Therefore, quartz can improve the ascharite flotation performance effectively by interaction between particles. Zeta potential tests showed the opposite charges on the surfaces of mineral particles, which was the basic reason leading to particles interaction. The force measurement results of AFM indicated that the attraction force existed between serpentine and ascharite, as well as serpentine and quartz, but the force between quartz and ascharite was repulsive. The DLVO theory was in a good agreement with the results of AFM. Eventually, particles coating were observed by SEM-EDS, which supported the results of DLVO theory and AFM measurements. During the flotation process, addition of quartz would lead to attachment of serpentine to the quartz surface, so the adverse effect of serpentine on decreasing floatability of ascharite was weakened.
Źródło:: Physicochemical Problems of Mineral Processing; 2017, 53, 2; 1161-1174
1643-1049
2084-4735
Pojawia się w:: Physicochemical Problems of Mineral Processing
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Selective flotation of siderite and quartz from a carbonate-containing refractory iron ore using a novel amino-acid-based collector
Autorzy:: Gu, X.
Zhu, Y.
Li, Y.
Han, Y.
Powiązania:: https://bibliotekanauki.pl/articles/110222.pdf
Data publikacji:: 2018
Wydawca:: Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:: flotation
quartz
hematite
siderite
refractory iron ores
molecular simulation
Opis:: A novel and highly-efficient amino-acid-based collector, α-ethylenediamine lauric acid (α-EDA-LA), was studied to selectively beneficiate carbonate-containing refractory hematite ores. Single mineral and synthetic mixture flotation tests were carried out to investigate its floating performance. Zeta potential, fourier transform infrared spectroscopy (FTIR) and Density Functional Theory-based molecular simulation were used to identify the adsorption mechanism. The flotation results showed that quartz could be collected effectively at pH 11.0-12.0 in the reverse flotation. For siderite, the recovery peaked at 83.4% at pH 8.0, where siderite presented different floatability from magnetite and hematite. Exploiting such difference, the separation of siderite could be achieved. Zeta-potential measurements showed that α-EDA-LA adsorption on the surfaces of siderite and quartz decreased the corresponding zeta potentials at pH of 8.0-10.0 and 8.0-12.0, respectively. This means the adsorption overcome the electrostatic repulsion between α-EDA-LA and the mineral surfaces. The molecular simulation indicated that no chemisorption took place between α-EDA-LA and quartz. FTIR analysis suggested that α-EDA-LA was adsorbed on quartz via hydrogen bonding. The adsorption of α-EDA-LA on siderite surface was dominated by chemisorption, while further enhanced by hydrogen bonding. This study filled the gap in the research on siderite flotation reagents and its adsorption mechanism.
Źródło:: Physicochemical Problems of Mineral Processing; 2018, 54, 3; 803-813
1643-1049
2084-4735
Pojawia się w:: Physicochemical Problems of Mineral Processing
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Novel roles for glutathione in linking oxidative stress to phytohormone-dependent signalling
Autorzy:: Noctor, G.
Han, Y.
Chaouch, S.
Mhamdi, A.
Powiązania:: https://bibliotekanauki.pl/articles/80088.pdf
Data publikacji:: 2013
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: conference
glutathione
oxidative stress
phytohormone
antioxidant
protein structure
protein function
signalling
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Effect of calcination temperature on activation behaviors of coal-series kaolin by fluidized bed calcination
Autorzy:: Yuan, S.
Han, Y.
Li, Y.
Gao, P.
Yu, J.
Powiązania:: https://bibliotekanauki.pl/articles/109800.pdf
Data publikacji:: 2018
Wydawca:: Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:: coal-series kaolin
fluidized bed calcination
calcination temperature
activation behaviors
Opis:: This paper is aimed at investigating activation behaviors for coal-series kaolin using fluidized bed calcination as a novel method. The properties of calcined products at different temperature by fluidized bed calcination were evaluated by determination of weight loss rate, whiteness, chemical oxygen demand (COD), aluminum dissolution degree. The thermal behaviors and reaction mechanism were characterized by thermo gravimetric-differential scanning calorimetry (TG-DSC), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), particle size distribution (PSD) and scanning electron microscopy (SEM). The results showed that calcination temperature was essential factor determining the properties and crystallinity of calcined products using fluidized bed calcination. Coal-series kaolin transformed into irregular and amorphous metakaolin with excellent properties as calcined at 600-900 °C, which attributed to the dehydroxylation of kaolinite and combustion of carbon/organic matter. Calcined kaolin eliminated activity ascribed to the recrystallization into mullite when calcination temperature was over 1000 °C. It was believed that fluidized bed calcination was an efficient thermal activation technology for coal-series kaolin and the calcination temperature should be controlled accurately.
Źródło:: Physicochemical Problems of Mineral Processing; 2018, 54, 2; 590-600
1643-1049
2084-4735
Pojawia się w:: Physicochemical Problems of Mineral Processing
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Flotation behavior and separation mechanism of quartz and iron minerals in α-bromolauric acid reverse flotation system
Autorzy:: Han, Y.
Guo, W.
Zhu, Y.
Wei, Y.
Gu, X.
Powiązania:: https://bibliotekanauki.pl/articles/949733.pdf
Data publikacji:: 2018
Wydawca:: Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:: quartz
separation
reverse flotation
iron minerals
α-Bromolauric acid
Opis:: A new type collector α-Bromolauric acid (α-BLA) had been proved to be an efficient collector for quartz flotation. However, the effects of α-BLA on the flotation behavior of iron minerals and quartz-iron separation had not been investigated. In this study, collector α-BLA was synthesized in the laboratory. The flotation behavior of quartz, hematite and magnetite under α-BLA reverse flotation system were investigated and the separation mechanism of quartz-irons was studied by contact angle, zeta-potential and infrared spectroscopy. The results showed that the optimum flotation pH was 11.5 for quartz, 6.45 for hematite and 6.97 for magnetite. The best α-BLA concentrations was 75 mg/dm3 for quartz flotation, 125 mg/dm3 for hematite and magnetite flotation. The activator CaCl2 had little effect on the flotation of hematite and magnetite, but the minimum dosage 50 mg/dm3 of activator CaCl2 was necessary for quartz flotation. On the contrary, starch had no effect on the flotation of quartz, while the recoveries of magnetite and hematite tended to be 0% as starch concentration more than 80 mg/dm3. The separation mechanism of quartz from iron minerals under α-BLA reverse flotation system was that the starch could be selectively adsorbed on the surface of hematite and magnetite in the form of strong hydrogen bond adsorption. However, the same adsorption of starch did not occur on the surface of quartz, so the α-BLA can be successfully adsorbed on the surface of activated quartz to make the quartz strongly hydrophobic, and then to be floated out.
Źródło:: Physicochemical Problems of Mineral Processing; 2018, 54, 3; 992-1003
1643-1049
2084-4735
Pojawia się w:: Physicochemical Problems of Mineral Processing
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: An innovative methodology for recycling iron from magnetic preconcentrate of an iron ore tailing
Autorzy:: Yu, J.
Han, Y.
Gao, P.
Li, Y.
Yuan, S.
Li, W.
Powiązania:: https://bibliotekanauki.pl/articles/110888.pdf
Data publikacji:: 2018
Wydawca:: Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:: magnetic preconcentrate
iron ore tailing
iron recycling
fluidized magnetization roasting
magnetic separation
Opis:: Iron ore tailing is a kind of hazardous solid waste produced by iron and steel industry. In order to separate and recycle iron from the magnetic preconcentrate of an iron ore tailing, an innovative technological route of fluidized magnetization roasting followed by low-intensity magnetic separation was proposed in this paper. The effects of roasting temperature, dosages of reducing gas CO and fluidizing gas N2 on recovery rate of iron were carried out and optimized. The results showed that the hematite was almost reduced to magnetite by a gas mixture of 4 m3/h CO and 1 m3/h N2 at roasting temperature of 540 °C. Under the optimized conditions, a magnetic concentrate assaying 61.4 wt% Fe with a recovery rate of 81.8% was obtained from the magnetic preconcentrate of an iron ore tailing. The iron chemical phase, X-ray diffraction (XRD), and optical metallographic microscope analyses revealed that siderite was converted to magnetite successfully after roasting, and some coarse magnetite-hematite interlocking particles were formed due to insufficient reaction time, which could also be recovered by magnetic separation after liberating from gangue minerals.
Źródło:: Physicochemical Problems of Mineral Processing; 2018, 54, 3; 668-676
1643-1049
2084-4735
Pojawia się w:: Physicochemical Problems of Mineral Processing
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Prediction of the height of overburden fractured zone in deep coal mining: case study
Prognozowanie wysokości strefy spękań w warstwach nadkładu w podziemnych kopalniach węgla: studium przypadku
Autorzy:: Han, Y.
Cheng, J.
Huang, Q.
Zou, D. H. S.
Zhou, J.
Huang, S.
Long, Y.
Powiązania:: https://bibliotekanauki.pl/articles/218745.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: wysokość strefy spękań w warstwie nadkładu
symulacje numeryczne
układ ortogonalny
pomiary in-situ
the hei ght of overburden fractured zone
numerical simulation
orthogonal design
in-situ test
Opis:: In the process of coal extraction, a fractured zone is developed in the overburden above the goaf. If the fractured zone is connected with an aquifer, then water inrush may occur. Hence, research and analysis of the height of overburden fractured zone (HOFZ) are of considerable significance. This study focuses on the HOFZ determination in deep coal mining. First, general deformation failure characteristics of overburden were discussed. Second, a new method, numerical simulation by orthogonal design(NSOD), have been proposed to determinate the HOFZ in deep coal mining. Third, the validity of NSOD is verified in the practical application, compared with empiric al formula in Chinese Regulations and in-situ test. These three methods were applied to determine the HOFZ of working face No. 111303 in No. 5 coal mine. The pre dicted HOFZ of NSOD is found to be similar to the result of the in-situ test (8.9% relative error), whereas the HOFZ calculated by the empirical formula has extremely large error (25.7% relative error). Results show that the NSOD can reliably predict the HOFZ in deep coal mining and reduce time and expenses required for in-situ test.
W trakcie prowadzenia wydobycia węgla w warstwie skalnej leżącej ponad zrobami powstaje strefa spękań. Jeśli nieciągłości te związane są z formacjami wodonośnymi, może nastąpić nagły wypływ wód do kopalni. Stąd też waga problemu i konieczność badania wysokości strefy spękań w warstwach nadkładu zalegających nad wyrobiskiem. W pracy tej główny nacisk położono na określenie wysokości strefy spękań warstwy nadkładu zalegającej ponad wyrobiskiem w kopalni podziemnej. W pierwszej części pracy przedstawiono główne charakterystyki powstawania deformacji i pęknięć w warstwach nadkładu. Następnie zaproponowano nową metodę symulacji numerycznych w układzie ortogonalnym i jej wykorzystanie do określania wysokości strefy spękań w warstwach nadkładu w kopalni podziemnej. Następnie powyższą metodę zweryfikowano w praktycznym zastosowaniu, jako poziomy odniesienia wykorzystano odpowiednie wzory empiryczne określone w przepisach obowiązujących w Chinach oraz wyniki pomiarów in-situ. Powyższe trzy metody zastosowane zostały do określenia wysokości strefy spękań w warstwach nadkładu przy prowadzeniu ściany 111303 w kopalni węgla nr 5. Wyniki obliczeń wysokości strefy spękań uzyskane w oparciu o zaproponowaną nową metodę w dużym stopniu pokrywały się z wynikami pomiarów in-situ (błąd względny 8.9%); podczas gdy obliczenia wysokości strefy spękań w oparciu o odpowiednie wzory empiryczne obarczone były dużym błędem (błąd względny 25.7%). Uzyskane wyniki wskazują wiarygodność obliczeń w oparciu o zaproponowaną metodę, co pozwoli na ograniczenie czasu i kosztów związanych z wykonywaniem pomiarów in-situ.
Źródło:: Archives of Mining Sciences; 2018, 63, 3; 617-631
0860-7001
Pojawia się w:: Archives of Mining Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Telomerase enhances osteogenic ifferentiation of sheep bone marrow mesenchymal stem cells (BMSCs) by up-regulating PI3K/Akt pathway in vitro
Autorzy:: Zhu, X.
Zhou, L.
Liu, Z.
Chen, X.
Wei, L.
Zhang, Z.
Liu, Y.
Zhu, Y.
Wang, Y.
Yang, X.
Han, Y.
Powiązania:: https://bibliotekanauki.pl/articles/2087304.pdf
Data publikacji:: 2020
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: bone marrow mesenchymal stem cells
telomerase
proliferation
osteogenic differentiation
PI3K/Akt signalling pathway
Źródło:: Polish Journal of Veterinary Sciences; 2020, 23, 3; 359-372
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Korean WA-DGNSS User Segment Software Design
Autorzy:: Shah, S. T.
Choi, W.S.
Han, W.Y.
Yun, H.
Kee, C.
Powiązania:: https://bibliotekanauki.pl/articles/116066.pdf
Data publikacji:: 2013
Wydawca:: Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:: global navigation satellite system (GNSS)
Global Positioning System GPS
User Segment
GPS Data Processing Classes
Opis:: Korean WA‐DGNSS is a large scale research project funded by Ministry of Land, Transport and Maritime Affairs Korea. It aims to augment the Global Navigation Satellite System by broadcasting additional signals from geostationary satellites and providing differential correction messages and integrity data for the GNSS satellites. The project is being carried out by a consortium of universities and research institutes. The research team at Electronics and Telecommunications Research Institute is involved in design and development of data processing softwares for wide area reference station and user segment. This paper focuses on user segment software design. Korean WA‐DGNSS user segment software is designed to perform several functions such as calculation of pseudorange, ionosphere and troposphere delays, application of fast and slow correction messages, and data verification. It is based on a layered architecture that provides a model to develop flexible and reusable software and is divided into several independent, interchangeable and reusable components to reduce complexity and maintenance cost. The current version is designed to collect and process GPS and WADGNSS data however it is flexible to accommodate future GNSS systems such as GLONASS and Galileo.
Źródło:: TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2013, 7, 1; 69-74
2083-6473
2083-6481
Pojawia się w:: TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: Oligonol prevented the relapse of dextran sulfate sodium-ulcerative colitis through enhancing Nrf2-mediated antioxidative defense mechanism
Autorzy:: Kim, K.-J.
Park, J.-M.
Lee, J.-S.
Kim, Y.S.
Kangwan, N.
Han, Y.-M.
Kang, E.A.
An, J.M.
Park, Y.K.
Hahm, K.-B.
Powiązania:: https://bibliotekanauki.pl/articles/69501.pdf
Data publikacji:: 2018
Wydawca:: Polskie Towarzystwo Fizjologiczne
Tematy:: ulcerative colitis
inflammatory bowel disease
oxidative stress
oligonol
polyphenol
adaptive response
quinone oxidoreductase
relapse
nuclear factor-kappaB
tumour necrosis factor-alpha
Źródło:: Journal of Physiology and Pharmacology; 2018, 69, 3
0867-5910
Pojawia się w:: Journal of Physiology and Pharmacology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 12.

Tytuł:: Flexible Software Design for Korean WA-DGNSS Reference Station
Autorzy:: Choi, W.S.
Chhattan, S.S.
Kye, J.E.
Han, W.Y.
Yun, H.
Kee, C.
Powiązania:: https://bibliotekanauki.pl/articles/116770.pdf
Data publikacji:: 2013
Wydawca:: Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:: Global Navigation Satellite System (GNSS)
Flexible Software Design
Wide area Reference Station (WRS)
Opis:: In this paper, we describe the software design results of WA‐DGNSS reference station that will be constructed in Korea in the near future. Software design of the WRS (Wide area Reference Station) is carried out by applying object oriented software methodology in order to provide flexibilities: easy of model change (namely ionospheric delay model etc) and system addition (Galileo, GLONASS in addition to GPS etc). Software design results include the use case diagrams for the functions to be executed, the architecture diagram showing components and their relationships, the activity diagrams of behaviors and models among them, and class diagrams describing the attribute and operation.
Źródło:: TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2013, 7, 1; 75-78
2083-6473
2083-6481
Pojawia się w:: TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 13.

Tytuł:: Characterization of Cladding Hull Wastes from Used Nuclear Fuels
Charakterystyka odpadów z okładzin reaktora wodnego ciśnieniowego
Autorzy:: Kang, K. H.
Lee, C. H.
Jeon, M. K.
Han, S. Y.
Park, G. I.
Hwang, S.-M.
Powiązania:: https://bibliotekanauki.pl/articles/357036.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: used nuclear fuels
metal waste
cladding hulls
zircaloy
radioactivity
reaktor wodny ciśnieniowy
odpady metalowe
odpady z okładzin
radioaktywność
Opis:: Used cladding hulls from pressurized water reactor (PWR) are characterized to provide useful information for the treatment and disposal of cladding hull wastes. The radioactivity and the mass of gamma emitting nuclides increases with an increase in the fuel burn-up and their removal ratios are found to be more than 99 wt.% except Co-60 and Cs-137. In the result of measuring the concentrations of U and Pu included in the cladding hull wastes, most of the residues are remained on the surface and the removal ratio of U and Pu are revealed to be over 99.98 wt.% for the fuel burn-up of 35,000 MWd/tU. An electron probe micro-analyzer (EPMA) line scanning shows that radioactive fission products are penetrated into the Zr oxide layer, which is proportional to the fuel burn-up. The oxidative decladding process exhibits more efficient removal ratio of radionuclides.
Źródło:: Archives of Metallurgy and Materials; 2015, 60, 2B; 1199-1203
1733-3490
Pojawia się w:: Archives of Metallurgy and Materials
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 14.

Tytuł:: Improved Mechanical Properties of Al-Si Alloys by Adding an Extrusion Process to Conventional Processing Method
Autorzy:: Han, J. Y.
Femi, O. E.
Kilicaslan, F.
Jeong, C. U.
Baeg, C. H.
Hong, S. J.
Koo, J. M.
Powiązania:: https://bibliotekanauki.pl/articles/355114.pdf
Data publikacji:: 2017
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Al-Si alloys
extrusion
mechanical properties
Opis:: In this study, the mechanical properties of Al-Si alloys was improved by selecting higher Si content and avoiding distortion in Al-Si matrix by adding an extrusion process such that the fabrication processes casting and extrusion were in sequence. Cast billets were extruded at ratios 4:1 and 10:1 to obtained two extruded bars with different processing parameters. The as-extruded samples were characterized and investigated for microstructure and mechanical properties. Optical Microscope was used to examined phase morphologies and microstructures of the extruded Al-Si bars. Mechanical properties were conducted on each sample to study the effectiveness of the additional extrusion process in high Si content Al-Si alloys. The result shows that by increasing the extrusion ratio, the size of the primary Si particle reduces leading to a higher density, better tensile and yield strength compared to the cast billet.
Źródło:: Archives of Metallurgy and Materials; 2017, 62, 2B; 1179-1183
1733-3490
Pojawia się w:: Archives of Metallurgy and Materials
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 15.

Tytuł:: Generation and characterization of a monoclonal antibody against duck Tembusu virus envelope protein
Autorzy:: Han, K.
Zhao, D.
Liu, Y.
Liu, Q.
Huang, X.
Yang, J.
Bi, K.
Xu, T.
Li, Y.
Powiązania:: https://bibliotekanauki.pl/articles/31495.pdf
Data publikacji:: 2016
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:: Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused massive economic losses to the duck industry in China. Envelope (E) protein of DTMUV is an important structural protein, which is able to induce protective immune response in target animals and can be used as specific serological diagnosis tool. In this study, a novel monoclonal antibody, designated mAb 3E9, was generated against DTMUV E protein. It is positive in indirect ELISA against both His-E protein and the purified whole viral antigen. Also, this mAb showed positive reaction with DTMUV in Western blot and indirect immunofluorescence assay, and the isotype was IgG1. End-point neutralizing assay performed in BHK-21 cells revealed that the neutralization titer of 3E9 against DTMUV JS804 strain reached 1:50. Furthermore, functional studies revealed that 3E9 blocks infection of DTMUV at a step on viral attachment. The anti-E mAbs produced in the present work may be valuable in developing an antigen-capture ELISA test for antigen detection or a competitive ELISA test for antibody detection or therapeutic medicine for DTMUV in poultry.
Źródło:: Polish Journal of Veterinary Sciences; 2016, 19, 4
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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