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    Wyświetlanie 1-6 z 6
    Tytuł:
    The dynamics of the surface layer of lipid membranes doped by vanadium complex : computer modeling and EPR studies
    Autorzy:
    Olchawa, R.
    Man, D.
    Pytel, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/147585.pdf
    Data publikacji:
    2015
    Wydawca:
    Instytut Chemii i Techniki Jądrowej
    Tematy:
    EPR probe
    lipid membrane
    membrane fluidity
    Monte Carlo simulation
    Opis:
    Penetration of the liposome membranes doped with vanadium complex formed in the liquid-crystalline phase from egg yolk lecithin (EYL) by the TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl) spin probes has been investigated. The penetration process was followed by 360 hours at 24◦C, using the electron spin resonance (EPR) method. The spectroscopic parameter of the partition (F) of this probe indicated that a maximum rigidity of the membrane was at 3% concentration of the vanadium complex. Computer simulations showed that the increase in the rigidity of the membrane corresponds to the closure of gaps in the surface layer of the membrane, and indicates the essential role of the membrane surface in transport processes.
    Źródło:
    Nukleonika; 2015, 60, No. 3, part 1; 395-398
    0029-5922
    1508-5791
    Pojawia się w:
    Nukleonika
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The impact of humic substances on the liposome structures : ESR method
    Autorzy:
    Man, D.
    Pisarek, I.
    Braczkowski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/147392.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Chemii i Techniki Jądrowej
    Tematy:
    EYL liposome
    ESR method
    humic substances
    Opis:
    In this paper the changes of membrane fluidity of liposome with additions of humic substances (humic and fulvic acids) were examined. Liposome were done by the sonication of lecithin EYL. Concentrations of humic substances in attitude to EYL varied between 0–10% of weight. The technique of electron spin resonance (ESR) were used for the examination followed by three spin probes with a variety placement of the membrane located. TEMPO probe melted in the hydrophobic membrane and in the aquatic solution which allowed to determine the spectroscopic partition parameter (F), indicating the changes that occur in water-lipid interphase. Probe 5-DOXYL placed directly under the heads of polar lipids and the order parameter measuring by the TII showed the changing of membrane fluidity at surface area. 16-DOXYL probe penetrated the middle of the lipid bilayer membrane and allowed to determine the rotational correlation time τ parameter, which gives us information about changing of the liquidity lipid bilayer. Studies showed that the tested humic substances significantly changed the membrane fluidity of liposome. The dynamics of this process depends on both: the fraction of humic substances and its quality and quantity as well as the placement area of the membrane.
    Źródło:
    Nukleonika; 2013, 58, 3; 439-442
    0029-5922
    1508-5791
    Pojawia się w:
    Nukleonika
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Impact of Plesiomonas shigelloides strain CNCTC 144/92 lipopolysaccharide on DPPC liposome membranes: EPR method
    Autorzy:
    Man, D.
    Pytel, B.
    Pisarek, I.
    Powiązania:
    https://bibliotekanauki.pl/articles/1054999.pdf
    Data publikacji:
    2017-07
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    87.16.Dg
    87.64.kh
    87.80.Lg
    Opis:
    Membrane fluidity measurements were performed for synthetic DPPC liposomes sonicated in aqueous solution and doped by Plesiomonas shigelloides strain CNCTC 144/92 (serotype O74:H5) lipopolysaccharide (LPS) extracted from the phenol (LPS_{PhOH}) and water (LPS_{H₂O}) phases. Concentrations of LPS in relation to DPPC ranged from 0 to 1.4% (molar ratio). The EPR spin probe method was used to describe physicochemical properties of different regions of the lipid bilayer. Since TEMPO spin probe dissolves both in the hydrophobic region of the membrane and in an aquatic environment it is possible to determine the spectroscopic partition parameter F, indicating the changes that occur in the water-lipid interface. The 16-DOXYL probe distributed in the middle of the lipid bilayer makes it possible to obtain the rotational correlation time τ parameter, which provides information about fluidity changes in the liposome membrane. Here we report that increasing concentrations (mainly in the range of 0.4-0.8%) of investigated LPS_{PhOH} and LPS_{H₂O} significantly influence spectroscopic parameters (F and τ). The surface area of the DPPC liposomes membranes was affected predominantly by LPS_{H₂O} while the lipid bilayer was most influenced by LPS_{PhOH}.
    Źródło:
    Acta Physica Polonica A; 2017, 132, 1; 77-80
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Impact of Vanadium(IV)-Oxy Acetylacetonate and Vanadium(III) Acetylacetonatel on the DPPC Liposome Membranes: EPR Studies
    Autorzy:
    Man, D.
    Mrówka, I.
    Wójcik, A.
    Pytel, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/1032414.pdf
    Data publikacji:
    2017-07
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    87.16.Dg
    87.64.kh
    87.80.Lg
    Opis:
    The effect of vanadium (IV)-oxy acetylacetonate (V4) and vanadium(III) acetylacetonate (V3) on the liposome membranes formed of synthetic lecithin (DPPC) was presented in this paper. Liposomes were formed during the sonication of DPPC lecithin in an aqueous medium. The concentration of the vanadium compounds changed in the range of 0% to 2.4% in molar ratio to the lecithin. The EPR technique made use of three spin probes penetrating the different areas of the membrane (as follows: TEMPO, 16-DOXYL stearic acid methyl ester, stearic acid 5-DOXYL methyl ester). TEMPO probe penetrates the interphase water-lipid (partition parameter F), the probe 16-DOXYL locates in the middle of the lipid bilayer (rotational correlation time τ), the probe 5-DOXYL gives a picture of membrane fluidity (the order parameter) just below the polar head groups. The results of our research showed the following conclusions. The change of membrane fluidity, as a function of admixture concentration, was dependent on the type of additives. TEMPO probe recorded an increase in liquidity interphase water-lipid for both admixtures: V3 and V4. 16-DOXYL probe indicated that the admixture V3 increases the fluidity in the center of the lipid bilayer. Admixture V4 significantly less impacted on the change of the membrane middle. The 5-DOXYL probe did not influence on the membrane surface portion, there were not observed significant changes under the impact of admixtures. V3 showed stronger impact on membrane fluidity.
    Źródło:
    Acta Physica Polonica A; 2017, 132, 1; 52-56
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of selected amino acids on the dynamic properties of the liposome membranes : ESR study
    Autorzy:
    Man, D.
    Broda, M.
    Buczek, A.
    Kawecka, A.
    Siodłak, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/146690.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Chemii i Techniki Jądrowej
    Tematy:
    EYL liposomes
    electron spin resonance (ESR) method
    N-methylation
    amino acids
    peptides
    Opis:
    In this work the changes in the fluidity of liposome membranes caused by alanine and butyrine derivatives (Ac-Ala-NMe2 and Ac-Abu-NMe2) were investigated. Liposomes were obtained in the process of egg yolk lecithin (EYL) sonication. The concentration of the admixture in the proportion to EYL varied from 0 to 25% mole. The electron spin resonance (ESR) spectroscopy was used with two different spins probes. Each spin probe penetrates different regions of liposome membrane. The TEMPO probe occurs both in the hydrophobic part of the membrane and in the water environment what allows to determine the spectroscopic parameter F of division of this probe into the membrane and its water surrounding. DOXYL is localized in the central part of the lipid bilayer and is used to obtain the spectroscopic parameter τ – rotation correlation time – whose value gives information about fluidity changes in the middle of the lipid bilayer. The study indicated that the tested as admixtures N-methylated model peptides significantly changed the fluidity of liposome membranes. The dynamic of this process depends both on amino acids derivative and on the membrane region. Both studied compounds increased the fluidity of the surface layer of liposome membrane. At the same time, butyrine derivative caused the stiffening of the middle part of liposome bilayer, but alanine derivative slightly increased the fluidity of this region.
    Źródło:
    Nukleonika; 2013, 58, 3; 443-446
    0029-5922
    1508-5791
    Pojawia się w:
    Nukleonika
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Impact of humic acids on EYL liposome membranes : ESR method
    Autorzy:
    Pytel, B.
    Filipiak, A.
    Pisarek, I.
    Olchawa, R.
    Man, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/146924.pdf
    Data publikacji:
    2015
    Wydawca:
    Instytut Chemii i Techniki Jądrowej
    Tematy:
    EYL liposomes
    ESR method
    humic substances
    Opis:
    In this paper, the effects of model (commercial) and natural (extracted from peat) humic substances on the membrane of liposomes formed with egg yolk lecithin (EYL) are presented. In our research, mass concentrations of fulvic and humic acids were used, which in relation to lecithin varied from 0% to 13%. To study membrane fluidity, electron spin resonance (EPR) was used with two spin probes, penetrating various regions of the lipid bilayer. The effects of model and natural humic substances (humic acids – HAs and fulvic acids – FAs) on the lipid membrane in different regions were researched: the lipid-water interphase, and in the middle of the lipid bilayer. It was shown that FA and HA impact the fluidity of liposome membranes in different ways. Increased mass concentrations of HAs decreased membrane fluidity in both acids: extracted from peat and the model. However, increased mass concentration of FAs extracted from peat, decreased membrane fluidity in the surface region, at the same time stiffening the central part of the bilayer. Increasing the concentration of FAs extracted from peat had the opposite effect when compared to model FA. This effect may be related to the complexation of xenobiotics present in the soil environment and their impact on biological membranes.
    Źródło:
    Nukleonika; 2015, 60, No. 3, part 1; 455-459
    0029-5922
    1508-5791
    Pojawia się w:
    Nukleonika
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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