Wszystkie pola: Beauveria bassiana - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Immunoliogical Responses of Hyphantria Cunea (Drury) (Lepidoptera: Arctiidae) to Entomopathogenic Fungi, Beauveria Bassiana (Bals.-Criy) and Isaria Farinosae (Holmsk.) Fr.
Autorzy:: Ajamhasssani, M.
Sendi, J.J.
Zibaee, A.
Askary, H.
Farsi, M.J.
Powiązania:: https://bibliotekanauki.pl/articles/66549.pdf
Data publikacji:: 2013
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:: Five morphological types of hemocytes were recognized in hemolymph of the 4th instar larvae of Hyphantria cunea (Drury). These hemocytes were: prohemocytes, plasmotocytes, granulocytes, oenocytoids, and spherulocytes. Tests were done on the effects of four isolates of the entomopathogenic fungus Beauveria bassiana (Bals.-Criy) (Fashand, spt-22, Ir-K-40 and 566), one isolate of Isaria farinosae (Holmsk.) Fr. (1872c), and latex-beads on the cellular immune defense mechanism and Phenoloxidase (PO) activity of H. cunea. Observation showed that plasmatocytes and granulocytes engulfed fungal pathogens by phagocytosis. The most phagocytosis occurred 30 and 60 min after injection but nodulation occurred in 3 and 6 hours, in all treatments. The total hemocyte count (THC) and granulocyte, and plasmotocyte numbers increased after the injection of spores. Phenoloxidase activity was determined in the presence of L-DOPA (L-3,4-dihydroxyphenylalanine), as a substrate in intervals, after injection of fungal spores, and latex beads. These studies demonstrated that B. bassiana is a promising candidate for biological control of H. cunea.
Źródło:: Journal of Plant Protection Research; 2013, 53, 2
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Purification and characterization of the cuticle-degrading proteases produced by an isolate of Beauveria bassiana using the cuticle of the predatory bug, Andrallus spinidens Fabricius (Hemiptera: Pentatomidae)
Autorzy:: Firouzbakht, H.
Zibaee, A.
Hoda, H.
Sohani, M.M.
Powiązania:: https://bibliotekanauki.pl/articles/65065.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:: The entomopathogenic fungi-like Beauveria bassiana must penetrate via the integument of an insect to reach the hemocoel. Since proteins are the molecules responsible for integument strength in insects, the proteins must synthesise the cuticle degrading proteases which will then enable the proteases to penetrate. It is important to determine the biochemical properties of these proteases so that fungal virulence can be better understood. In the current study, a recently collected isolate of B. bassiana, namely AM-118, was inoculated in liquid media containing 0.5% of Andrallus spinidens Fabricus cuticle to obtain specific proteases. The crude samples were purified via a three step process using ammonium sulfate, Sepharyl G-100, and DEAE-Cellulose Fast Flow. The results revealed two proteases known as subtilisin-like (Pr1), and trypsin-like (Pr2), with the molecular weights of 105 and 103 kDa. The optimal pH and temperature values were found to be 8 and 35°C for Pr1 and 8 and 40°C for Pr2, respectively. Inhibitors like AEBSF, EDTA, TPCK, and phenanthroline significantly affected proteolytic activities. Here, we reported two fungal proteases by high molecular weight from an Iranian isolate of B. bassiana. These findings will help us to better understand fungal virulence against insects.
Źródło:: Journal of Plant Protection Research; 2015, 55, 2
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Changes in cellular immune responses of Chilo suppressalis Walker (Lepidoptera: Crambidae) due to pyriproxyfen treatment
Autorzy:: Mirhaghparast, S.K.
Zibaee, A.
Hoda, H.
Sendi, J.J.
Powiązania:: https://bibliotekanauki.pl/articles/66690.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: cellular immune response
Chilo suppressalis
Asiatic rice borer
striped rice stemborer zob.Asiatic rice borer
Lepidoptera
Crambidae
pyriproxyfen
treatment
Beauveria bassiana
Opis:: The effects of pyriproxyfen were determined on the cellular immunity and phenoloxidase activity in the 4th instar larvae of Chilo suppressalis Walker. The bioassay results revealed the effective concentrations of: 10L : 18C, 30L : 72C and 50L : 190C μg · ml–1. The sole effect of 18 and 72 μg · ml–1 concentrations at intervals of 1–3 h caused a higher number of total hemocytes in the treated larvae than the control, but the reverse results were observed after 6–24 h. The number of plasmatocytes was lower than that of the control for intervals of 3–24 h but the number of granulocytes was higher than the control after 1–3 h although no significant differences were observed at the other times. In the treated larvae, the activities of phenoloxidase were higher and lower than those of the control after 1–3 h and 6–24 h, respectively. The combined effects of pyriproxyfen and the entomopathogenic fungus, Beauveria bassiana isolate B3 caused higher numbers of total hemocytes, plasmatocytes, and granulocytes in the treated larvae by use of the three concentrations of pyriproxyfen, at intervals of 6 and 12 h. Although the numbers of nodules in the larvae treated with concentrations of 18 μg · ml–1 were higher than those of other treatments, the overall numbers were lower than those of the control. Finally, the activity of phenoloxidase in the treated larvae was higher than that of the control, at intervals of 6 and 12 h post-treatment. Findings of the current study indicate an intervening role of pyriproxyfen in the cellular immunity of C. suppressalis to entomopathogenic objects.
Źródło:: Journal of Plant Protection Research; 2015, 55, 3
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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