- Tytuł:
- Biochemical characterization of a catalase from Vibrio vulnificus, a pathogen that causes gastroenteritis
- Autorzy:
-
Pei, Jihua
Wang, Haijun
Wu, Limin
Xia, Shenglong
Xu, Changlong
Zheng, Bo
Li, Tianya
Jiang, Yi - Powiązania:
- https://bibliotekanauki.pl/articles/1038620.pdf
- Data publikacji:
- 2017
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
catalase
Vibrio vulnificus
kinetics
mutation
gastroenteritis. - Opis:
- Vibrio vulnificus is a virulent human pathogen causing gastroenteritis and possibly life threatening septicemia in patients. Most V. vulnificus are catalase positive and can deactivate peroxides, thus allowing them to survive within the host. In the study presented here, a catalase from V. vulnificus (CAT-Vv) was purified to homogeneity after expression in Escherichia coli. The kinetics and function of CAT-Vv were examined. CAT-Vv catalyzed the reduction of H2O2 at an optimal pH of 7.5 and temperature of 35°C. The Vmax and Km values were 65.8±1.2 U/mg and 10.5±0.7 mM for H2O2, respectively. Mutational analysis suggests that amino acids involved in heme binding play a key role in the catalysis. Quantitative reverse transcription-PCR revealed that in V. vulnificus, transcription of CAT-Vv was upregulated by low salinity, heat, and oxidative stresses. This research gives new clues to help inhibit the growth of, and infection by V. vulnificus.
- Źródło:
-
Acta Biochimica Polonica; 2017, 64, 3; 543-549
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł