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Wyszukujesz frazę "Xin, Li" wg kryterium: Autor


Wyświetlanie 1-3 z 3
Tytuł:
High-grade mutant OmpF induces decreased bacterial survival rate
Autorzy:
Zhao, Zhi-ping
Liu, Ting-ting
Zhang, Li
Luo, Min
Nie, Xin
Li, Zai-xin
Pan, Yu
Powiązania:
https://bibliotekanauki.pl/articles/1039304.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
antibiotic resistance
mOmpF
OmpF
outer membrane protein
bacterial survival rate
Opis:
OmpF plays very important roles in the influx of antibiotics and bacterial survival in the presence of antibiotics. However, high-grade mutant OmpF and its function in decreasing bacterial survival rate have not been reported to date. In the present study, we cloned a high-grade mutant OmpF (mOmpF) and sequence analysis suggested that over 45 percent of the DNA sequence was significantly mutated, leading to dramatic changes in over 55 percent of the amino acid sequence. mOmpF protein was successfully expressed. When grown in the presence of antibiotic, the bacterial survival rate decreased and the antibiotic inhibition zone became larger with the increase of the mOmpF. It was concluded that concentration of high-grade mutant mOmpF dramatically influenced the bacterial survival rate. The study presented here may provide insights into better understanding of the relationships between structure and function of OmpF.
Źródło:
Acta Biochimica Polonica; 2014, 61, 2; 369-373
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Human neutrophil peptide 3 could be functionally expressed in Rhodobacter sphaeroides
Autorzy:
Nie, Xin
Zhang, Li
Hu, Zongli
Liu, Yi
Zhao, Zhiping
Powiązania:
https://bibliotekanauki.pl/articles/1039101.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Rb. sphaeroides
HNP3
expression system
antimicrobial activity
spectral absorption
Opis:
Human neutrophil peptides (HNPs) possess high antimicrobial activities against a broad spectrum of microorganisms. Rhodobacter sphaeroides is the best-characterized photosynthetic bacterium and exhibits potential as a novel expression system. Up to date, no literature has been reported regarding expression of HNP3 in Rb. sphaeroides. In the present study, the HNP3 gene fragment was amplified by SOE PCR and ligated into photosynthetic bacteria light-harvesting complex 2 (LH2) expression vector leading to HNP3 fusion protein expression vector. The HNP3 fusion protein was successfully expressed as rapidly evaluated by the LH2 characteristic peaks at ~800 nm and ~850 nm before purification and SDS/PAGE. Subsequently, the HNP3 fusion protein was purified by one-step affinity chromatography, and could be rapidly detected by the color and the spectral absorption at ~800 nm and ~850 nm before SDS/PAGE. Antimicrobial activity assay suggested that the HNP3 fusion protein exhibited high antimicrobial activity towards E. coli. The present study may supply an insight into employing the novel Rb. sphaeroides expression system, exhibiting dramatic advantages over currently used commercial expression system, to heterologously express human neutrophil peptides.
Źródło:
Acta Biochimica Polonica; 2015, 62, 2; 259-263
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Strain measurement based on fixed wavelength transmission of tapered long-period fiber grating
Autorzy:
Tang, Yong
Zhang, Hao
Zhang, Li
Yu, Xin Feng
Bai, Yun Feng
Powiązania:
https://bibliotekanauki.pl/articles/2172862.pdf
Data publikacji:
2022
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
tapered fiber
long-period fiber
fiber grating
strain sensor
fixed dual-wavelength ratio
Opis:
This paper studies the relationship between transmission intensity and strain based on tapered long-period fiber grating at a fixed wavelength. In experiments, tapered long-period fiber grating was prepared by the electric melting method. Experimental results show that two resonance peaks appeared at 1482 and 1537 nm, respectively. Here is the elaboration of the relationship between the resonant wavelength and the strain, its wavelength-strain sensitivity is 20 pm/με, and the linearity was negative. Then our next study was about the relationship between transmission intensity and strain at a fixed wavelength. The results show that the transmission intensity at a fixed wavelength is related to the exponent with strain. The coupled-mode theory is applied to simulate the relationship between fixed wavelength and strain. The simulation results matched the experimental results. Two fixed wavelength transmission intensity ratio was used, and the ratio showed a linear relationship with the strain, and the slope is –0.018 dB/με. Therefore, within the 0.01% resolution of our detector, we could resolve a 0.16 με strain change. We can select the appropriate light source and detector to achieve higher measurement accuracy. Thus, there is a great potential in fiber grating strain sensors.
Źródło:
Optica Applicata; 2022, 52, 4; 511--519
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-3 z 3

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