Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

English (EN)·Polski (PL)·Yкраїнський (UK)
Przejdź do strony domowej biblioteki Centralna Biblioteka Wojskowa Link otwiera się w nowym oknie Logo biblioteki Prolib Integro - strona głównaCentralna Biblioteka Wojskowa

Wyszukujesz frazę "Zhang, Z." wg kryterium: Autor

  Lista filtrów
Wyrównaj
    Wyświetlanie 1-14 z 14
    Tytuł:
    Analysis of recent coastline evolution due to marine reclamation projects in the Qinzhou Bay
    Autorzy:
    Yan, Y.
    Zhang, Z.
    Wang, C.
    Zhang, L.
    Huang, Y.
    Zhang, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/258862.pdf
    Data publikacji:
    2017
    Wydawca:
    Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
    Tematy:
    Qinzhou Bay
    marine reclamation
    remote sensing
    geospatial analysis
    coastline evolution
    Opis:
    Intense marine reclamation activities bring obvious benefits, meanwhile, these activities also influence the coastal natural formation, hydrodynamic processes and water environment etc. In order to investigate the exact influence in the Qinzhou Bay brought by marine reclamation behaviors in recent years, based on the remote sensing image data in the Qinzhou Bay in 2006, 2008, 2009, 2012 and 2014, with the help of GIS and RS software, the thesis interprets Qinzhou Bay coastline and artificial reclamation by man-machine interaction and makes quantitative analysis in the Qinzhou Bay coast evolution recently. The results show: (1) Qinzhou Bay coastal morphology change degree was strong in 2006 - 2012 and the coastline changed greatly. Originally part of the natural shore segments which had twists and turns were gradually filled into artificial coast, the coast constantly advanced to the sea, coastline became regular and straight; (2) The change degree of coastline was relatively small in 2006-2008, the average artificial coastline increased 8.86 km/a, the average artificial reclamation area was 20.79 km2 /a; in 2008-2012, the change range of coastline was the greatest, coastline changed most severely, the average artificial coastline increased 16.07 km/a, the average artificial reclamation area was 58.52 km2 /a; however, in 2012-2014, coastline change degree relatively slowed, the average artificial coastline increased 4.04 km per year, the average artificial reclamation area was 3.464 km2 /a. (3) Compared to the studies of predecessors, the innovation of this paper is that it is the first to carry out the detailed research on coastline evolution caused by reclamation engineerings of the Qinzhou Bay, then formed the change results on coastline that caused by intense human activities in the Qinzhou Bay, provided the area of the marine environment protection with full and accurate data.
    Źródło:
    Polish Maritime Research; 2017, S 2; 188-194
    1233-2585
    Pojawia się w:
    Polish Maritime Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Research of the Self-Restoration Speed [-5pt]of Low Remanence Toroid Cores
    Autorzy:
    Huang, Z.
    Zhang, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/1807990.pdf
    Data publikacji:
    2009-06
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    01.30.Cc
    75.60.Ej
    Opis:
    Theory analysis and experiments have been done to find out the factors which decide on the self-restoration speed of low remanence toroid cores. The results show that in the right working circuit, the core with appropriate pulse permeability can be restored to its remanence after excitation within 1 μs without reset current. Therefore, the toroid core restoration in a MHz repeat frequency, multi-pulse induction cavity is likely to be solved by choosing the right material of cores and use proper working circuit.
    Źródło:
    Acta Physica Polonica A; 2009, 115, 6; 1189-1191
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparative experiment study on nitrogen injection and free desorption of methane-rich bituminous coal under triaxial loading
    Eksperymenty i badania porównawcze efektów wstrzykiwania azotu i swobodnej desorpcji na węglu bitumicznym o wysokiej zawartości metanu w warunkach obciążeń trójosiowych
    Autorzy:
    Zhang, L.
    Ye, Z.
    Tang, J.
    Hao, D.
    Zhang, C.
    Powiązania:
    https://bibliotekanauki.pl/articles/219975.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    wstrzykiwanie gazu
    metan
    tempo wypierania
    swobodna desorpcja
    gas injection
    methane
    displacement ratio
    free desorption
    Opis:
    As a kind of associated geological gas, coalbed methane (CBM) is mainly adsorbed in the coal seam. The coal-methane adsorption phenomenon can be described by Langmuir monolayer adsorptio n model, BET multilayer adsorption model and the Theory of Volume Filling of Micropore (TVFM), whereas the binary gas adsorption phenomenon can be described by the extended Langmuir Model. For the CBM in the low permeability coal seam, the amount of gas released by direct drainage is relatively limited, which cannot eliminate the gas explosion and outburst hazards. Gas injection is an effective method to promote methane drainage. In this paper, the free desorption and nitrogen injection displacement experiments are comparatively analyzed, which allows verifying the effectiveness of nitrogen injection’s enhancement to gas drainage. The experiment of injecting nitrogen gas into the coal body shows that the coal fracture can be maintained or expanded by the injected gas pressure so that more methane can be released. The nitrogen injection has a higher time efficiency than that of free desorption as well. The displacement ratio of N2/CH4 is in the range of 1-3. Both the injection pressure and confining pressure affect the displacement ratio. The analysis of the desorbed gas components shows that the relationship between the methane component and gas flooding time is an “inverted S” shape curve, and the appropriate time for the methane collection can be inferred by the time interval of the rapid decline of the curve.
    Metan zawarty w złożu węgla występuje w głównej mierze w postaci zaadsorbowanej, jako gaz związany. Zjawisko adsorpcji metanu na węglu opisuje model adsorpcji jednowarstwowej Langmuira, model adsorpcji wielowarstwowej BET oraz teoria objętościowego wypełniania mikroporów. Z kolei procesy desorpcji mieszanin dwóch gazów opisuje rozszerzony model Langmuira. W przypadku metanu zawartego w złożu węgla o niskiej przepuszczalności, ilość gazu uwalnianego poprzez proste odgazowanie jest ograniczona i wciąż nie eliminuje ryzyka wybuchu gazu oraz wyrzutu skał i gazu. Wstrzykiwanie gazów jest jedną ze skutecznych metod odgazowania. W pracy tej przeprowadzono badanie porównawcze rezultatów swobodnej desorpcji gazu oraz wstrzyknięcia do złoża azotu, w celu zademonstrowania skuteczności odgazowania poprzez wprowadzenie azotu. Eksperyment polegający na wstrzykiwaniu azotu do calizny węglowej pokazuje możliwość zachowania lub powiększenia pęknięć w strukturze węgla pod działaniem ciśnienia wstrzykiwanego gazu, co umożliwi uwolnienie większych ilości metanu. Ponadto, wprowadzenie azotu okazuje się rozwiązaniem skuteczniejszym w dłuższym okresie czasu niż swobodna desorpcja. Stosunek wypierającego metan N2 do CH4 wynosi w przybliżeniu 1:3. Zarówno ciśnienie wstrzykiwanego gazu jak i wielkość ciśnienia złożowego mają wpływ na tempo wypierania metanu przez azot. Analiza składników desorbowanego gazu wskazuje, że zależność ilości metanu od czasu wypływu gazu opisywana jest na wykresie krzywą w postaci odwróconej litery S, zaś odpowiedni czas na odzysk metanu odczytujemy jako przedział odpowiadający nagłemu obniżeniu widocznemu na wykresie.
    Źródło:
    Archives of Mining Sciences; 2017, 62, 4; 911-928
    0860-7001
    Pojawia się w:
    Archives of Mining Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Decay of Electric Charge on Corona Discharge Polyethylene
    Autorzy:
    Zhang, L.
    Xu, Z.
    Chen, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/1807998.pdf
    Data publikacji:
    2009-06
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    52.80.-s
    81.05.Lg
    84.30.Jc
    Opis:
    In this paper, surface potential decay after corona charge deposition has been investigated for low density polyethylene (LDPE) films. We believe that the three ways of electron charge decay on the surface of insulating polyethylene film is reasonable. The factor of neutralizing with opposite charge in air is negligible, and leakage along insulator surface or through the body is much more important for electron charge decay. Both ways are related with surface voltage of the sample. When the voltage is lower than a critical value, surface leakage contributes most. If the voltage is higher than this value, the electron energy on the surface of the sample exceeds the critical value and it is easy to cross the "deep trap". In this situation, the body leakage is more important, and the decay on the surface of sample speeds. That is why there exists decay curve crossing phenomenon.
    Źródło:
    Acta Physica Polonica A; 2009, 115, 6; 1197-1200
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Robust stability of a family of matrices
    Autorzy:
    Wang, L.
    Zhang, L.
    Wang, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/205534.pdf
    Data publikacji:
    2001
    Wydawca:
    Polska Akademia Nauk. Instytut Badań Systemowych PAN
    Tematy:
    rezultat wierzchołkowy
    rodzina macierzy
    stabilność odporności
    struktura wieloliniowej niepewności
    teoria algebry
    algebra theory
    matrix families
    multilinear uncertainty structure
    robust stability
    vertex results
    Opis:
    For a family of matrices with multilinear uncertainty structure, stability of the entire matrix family can be guaranteed by stability of its vertex matrices.
    Źródło:
    Control and Cybernetics; 2001, 30, 3; 331-337
    0324-8569
    Pojawia się w:
    Control and Cybernetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Contribution on fluid inclusion abundance to activation of quartz flotation
    Autorzy:
    Yuan, Y.
    Zhang, L.
    Guan, J.
    Zhang, C.
    Wu, J.
    Chen, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/110067.pdf
    Data publikacji:
    2018
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    recovery
    flotation
    mineralogy
    fluid Inclusions
    fluid inclusion abundance
    Opis:
    In this study, comparative experiments were conducted on the recovery of quartz using flotation with different fluid inclusion abundances. A large number of fluid inclusions with various sizes have been found in natural quartz. Micrographs, inductively coupled plasma, electron probe microanalysis, homogenization temperature, Raman spectra, zeta potentials, X-ray photoelectron spectroscopy, scanning electron microscope, and energy dispersive spectrometer were used to characterize the fluid inclusions and quartz, as well as the adsorption tests and single mineral flotation experiments to investigate its floatability. The results demonstrated that it was more likely for quartz with higher fluid inclusion abundance to connect with Fe3+ sufficiently to achieve a high level of flotation recovery, due to the powerful collecting ability by sodium dodecyl sulphonate to Fe3+. Furthermore, the mechanism indicated that the adsorption between quartz and Fe3+ was a process of chemisorption.
    Źródło:
    Physicochemical Problems of Mineral Processing; 2018, 54, 3; 981-991
    1643-1049
    2084-4735
    Pojawia się w:
    Physicochemical Problems of Mineral Processing
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    CO2 concentration affects in vitro pig embryo developmental capacity
    Autorzy:
    Zhang, L.
    Lin, Z.
    Bi, Y.
    Zheng, X.
    Xiao, H.
    Hua, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087657.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    CO2
    developmental capacity
    pig embryos
    in vitro
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 3; 609-614
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Light Effect in Semiconductor Bridge Plasma Ignition
    Autorzy:
    Zhang, L.
    Li, N.
    Wan, Z.
    Zhu, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/358252.pdf
    Data publikacji:
    2017
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
    Tematy:
    SCB plasma ignition
    non-heat effect
    light effect
    free radicals
    Opis:
    Heat is considered to play an important role in Semiconductor Bridge (SCB) plasma ignition. Nevertheless, in this paper a non-heat effect is reported for SCB ignition of primary explosives. An initial comparison showed that there is no reasonable correlation between the ease of plasma ignition and the 5-s explosion temperature. Meanwhile the addition of Pb3O4 was found to make lead styphnate (LS) more active to SCB plasma ignition whereas the heat decomposition of this mixture was not accelerated. In terms of the phenomena mentioned above and the response of primary explosives to SCB plasma, we propose an effect of light in SCB plasma ignition. The free radical concentration change indicates that light enhances the activity of primary explosives in SCB plasma ignition. Regarding the mixture of LS and Pb3O4, the additive itself does not make LS sensitive to the SCB plasma. However, the supplement makes LS active under light exposure. As a result, the effect of light on SCB plasma ignition was confirmed by the experiments conducted in this study. This paper provides a new understanding of SCB plasma ignition from the viewpoint of explosives, which is of importance for the design of SCBs.
    Źródło:
    Central European Journal of Energetic Materials; 2017, 14, 4; 996-1006
    1733-7178
    Pojawia się w:
    Central European Journal of Energetic Materials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Analytical Descriptions of Dynamic Softening Mechanisms for Ti-13Nb-13Zr Biomedical Alloy in Single Phase and Two Phase Regions
    Autorzy:
    Quan, G.-Z.
    Wang, X.
    Li, Y.-L.
    Zhang, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/353917.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    biomedical titanium alloy
    flow stress
    dynamic recovery
    dynamic recrystallization
    dynamic softening
    Opis:
    Dynamic softening behaviors of a promising biomedical Ti-13Nb-13Zr alloy under hot deformation conditions across dual phase α + β and single phase β regions were quantitatively characterized by establishing corresponding dynamic recovery (DRV) and dynamic recrystallization (DRX) kinetic models. A series of wide range hot compression tests on a Gleeble-3500 thermo-mechanical physical simulator were implemented under the strain rate range of 0.01-10 s−1 and the temperature range of 923-1173 K. The apparent differences of flow stress curves obtained in dual phase α + β and single phase β regions were analyzed in term of different dependence of flow stress to temperature and strain rate and different microstructural evolutions. Two typical softening mechanisms about DRV and DRX were identified through the variations of a series of stress-strain curves acquired from these compression tests. DRX is the dominant softening mechanism in dual phase α + β range, while DRV is the main softening mechanism in single phase β range. The DRV kinetic model for single phase β region and the DRX kinetic model for dual phase α + β region were established respectively. In addition, the microstructures of the compressed specimens were observed validating the softening mechanisms accordingly.
    Źródło:
    Archives of Metallurgy and Materials; 2017, 62, 4; 2029-2043
    1733-3490
    Pojawia się w:
    Archives of Metallurgy and Materials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A new adaptation for in vitro co-culture of single porcine parthenogenetic embryos using feeder cells
    Autorzy:
    Zhang, L.
    Lin, Z.
    Hua, Z.
    Zheng, X.
    Xiao, H.
    Hua, W.
    Ren, H.
    Zhu, Z.
    Molenaar, A.
    Bi, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087368.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    feeder cells
    porcine
    embryos
    in vitro
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 4; 711-716
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Structural Stabilities and Elastic Constants of EuX (X = O, S, Se, and Te) Compounds at High Pressure
    Autorzy:
    Kong, B.
    Zhou, Z.
    Sun, X.
    Zhang, L.
    Ling-Hu, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/1400144.pdf
    Data publikacji:
    2013-04
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    61.66.-f
    46.70.-p
    61.43.-j
    75.50.-y
    Opis:
    The structural stabilities, elastic properties and charge transfers of EuX (X = O, S, Se, Te) compounds as a function of pressure are investigated extensively using first-principles calculations. The ground-state parameters, such as lattice constants, bulk modulus are predicted and compared with the available data, our results are satisfactory. The calculated phase transition pressures from the NaCl-type (B1) structure to the CsCl-type (B2) structure for EuX (X = O, S, Se, Te) also accord with the experiments. Particularly, the elastic constants of EuX (X = O, S, Se, Te) under zero pressure and high pressure are simulated appropriately for the first time via density functional theory. The softening behaviors of the elastic shear modulus $C_{44}$ under pressure for the B1 phase of EuX (X = O, S, Se, Te) are captured, which should be responsible for the pressure-induced structural phase transition in the EuX system. It is also suggested that the softening behavior might be induced partly by the p → d and f → d electron transfers of Eu atom under pressure. In addition, the aggregate elastic modulus (B, G, E), Poisson's ratio (σ), Debye temperature $\Theta p_{D}$ are also successfully obtained for both B1 and B2 phases of EuX.
    Źródło:
    Acta Physica Polonica A; 2013, 123, 4; 720-727
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Seroprevalence of antibodies to classical swine fever virus and porcine reproductive and respiratory syndrome virus in healthy pigs in Hunan Province, China
    Autorzy:
    Yu, H.
    Zhang, L.
    Cai, Y.
    Hao, Z.
    Luo, Z.
    Peng, T.
    Liu, L.
    Wang, N.
    Wang, G.
    Deng, Z.
    Zhan, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/16539345.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    classical swine fever virus (CSFV)
    porcine reproductive and respiratory syndrome virus (PRRSV)
    pig
    seroprevalence
    antibody
    Opis:
    Classical swine fever (CSF) and porcine reproductive and respiratory syndrome (PRRS) are responsible for major economic losses and represent a threat to the swine industry worldwide. Routine surveillance serology for CSF and PRRS viruses is critical to maintaining the health status of sow farms in Hunan Province, which is one of the top pig production provinces in China. The aim of our study was to investigate the serological statistics of CSF virus (CSFV) and PRRS virus (PRRSV) in Hunan Province. The cohort serum samples were collected from vaccinated and unvaccinated pigs. Our findings showed that the average rates of CSFV and PRRSV antibody seropositivity were 82.2% (95% CI: 80.1-84.3) and 84.8% (95% CI: 82.5-87.1), respectively, in the immunized group and that these rates were higher than those in the unvaccinated group (58.6% for CSFV and 47.8% for PRRSV). Additionally, the level of CSFV antibody in piglet serum declined gradually with age, whereas PRRSV-specific antibody level increased initially (1 to 2 weeks old) and then declined with age (2 to 4 weeks old). In summary, we investigated the difference in CSFV/PRRSV antibody levels among piglets at various weeks old (1 to 4 weeks) to further establish the duration of maternal immunity in piglets. In addition, routine monitoring of CSFV/PRRSV antibodies in immunized pigs was carried out to evaluate the efficacy of vaccination.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 3; 375-381
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Weighted prediction method with multiple time series using multi-kernel least squares support vector regression
    Metoda ważonej predykcji wielokrotnych szeregów czasowych z wykorzystaniem wielojądrowej regresji wektorów wspierających metodą najmniejszych kwadratów (LS-SVR)
    Autorzy:
    Guo, Y. M.
    Ran, C. B.
    Li, X. L.
    Ma, J. Z.
    Zhang, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/302067.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Polskie Naukowo-Techniczne Towarzystwo Eksploatacyjne PAN
    Tematy:
    szereg czasowy
    predykcja ważona
    regresja wektorów wspierających metodą najmniejszych kwadratów (LS-SVR)
    uczenie wielojądrowe (MKL)
    time series
    weighted prediction
    least squares support vector regression (LS-SVR)
    multiple kernel learning (MKL)
    Opis:
    Least squares support vector regression (LS-SVR) has been widely applied in time series prediction. Based on the case that one fault mode may be represented by multiple relevant time series, we utilize multiple time series to enrich the prediction information hiding in time series data, and use multi-kernel to fully map the information into high dimensional feature space, then a weighted time series prediction method with multi-kernel LS-SVR is proposed to attain better prediction performance in this paper. The main contributions of this method include three parts. Firstly, a simple approach is proposed to determine the combining weights of multiple basis kernels; Secondly, the internal correlative levels of multiple relevant time series are computed to present the different contributions of prediction results; Thirdly, we propose a new weight function to describe each data's different effect on the prediction accuracy. The experiment results indicate the effectiveness of the proposed method in both better prediction accuracy and less computation time. It maybe has more application value.
    Regresja wektorów wspierających metodą najmniejszych kwadratów (LS-SVR) jest szeroko stosowana w predykcji szeregów czasowych. Opierając się na fakcie, że jeden rodzaj niezdatności może być reprezentowany przez wiele relewantnych szeregów czasowych, w niniejszej pracy wykorzystano wielokrotne szeregi czasowe do wzbogacenia informacji predykcyjnych ukrytych w szeregach czasowych oraz posłużono się metodą uczenia wielojądrowego (multi-kernel) w celu mapowania informacji do wysoko wymiarowej przestrzeni cech, a następnie zaproponowano metodę ważonej predykcji wielokrotnych szeregów czasowych z wykorzystaniem wielojądrowej regresji LS-SVR służącą osiągnięciu lepszej wydajności prognozowania.Metoda składa się z trzech głównych części. Po pierwsze, zaproponowano prosty sposób określania łącznej wagi wielu jąder podstawowych. Po drugie, obliczono wewnętrzne poziomy korelacyjne wielokrotnych szeregów czasowych w celu przedstawienia różnego udziału wyników prognozowania. Po trzecie, zaproponowano nową funkcję wagi do opisu różnego wpływu poszczególnych danych na trafność predykcji. Wyniki doświadczenia wskazują na skuteczność proponowanej metody zarówno jeśli chodzi o lepszą trafność predykcji jak i krótszy czas obliczeniowy. Proponowane rozwiązanie ma potencjalnie dużą wartość aplikacyjną.
    Źródło:
    Eksploatacja i Niezawodność; 2013, 15, 2; 188-194
    1507-2711
    Pojawia się w:
    Eksploatacja i Niezawodność
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Impact of different sources of donor cells upon the nuclear transfer efficiency in Chinese indigenous Meishan pig
    Autorzy:
    Hua, Z.
    Xu, G.
    Liu, X.
    Bi, Y.
    Xiao, H.
    Hua, W.
    Li, L.
    Zhang, L.
    Ren, H.
    Zheng, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/31224.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Somatic cell nuclear transfer (SCNT) is currently the most efficient and precise method to generate genetically tailored pig models for both agricultural and biomedical research. However, its efficiency is crucially dependent on the source of nuclear donor cells. In this study, we compared the cloning efficiency by using three lines of donor cells that are derived from fetal, newborn and adult fibroblasts of Chinese indigenous Meishan pig. We showed that cleavage rate and blastocyst formation rate of the reconstructed embryos were not significantly different between the fetal (80.7% and 15.6%) and newborn ear skin (77.5% and 12.3%) fibroblast groups (p>0.05), but in both groups these indices were significantly higher than that found in the adult ear skin (70.5% and 8.8%; p<0.05). Reconstructed embryos derived from fetal, newborn, and adult ear skin fibroblasts were transferred to four surrogates, respectively. For the fetal, newborn, and adult ear skin fibroblasts, the number of pregnancies were two (50.0%), two (50.0%), and one (25.0%), respectively, and the number of deliveries were two (50.0%), one (25.0%), and zero (0.0%), respectively. Seven and two cloned piglets were obtained from the fetal and newborn ear skin fibroblasts respectively, while no piglets were obtained from the adult ear skin fibroblasts. Two cloned piglets from the newborn ear skin fibroblasts died shortly after birth because of neonatal asphyxia caused by dystocia. The birth weights of the piglets derived from the fetal and newborn ear skin fibroblasts were 1230.5 and 1310.0g, respectively, which were statistically insignificant (p>0.05), but both were significantly higher than that of the control groups (p<0.05). Microsatellite analyses demonstrated that the genotypes of all cloned piglets were identical to their donor cells. Therefore, cloned pigs were successfully produced using two sources of donor cells isolated from the fetal and newborn ear skin fibroblasts of Meishan piglet, and indicating a better cloning efficiency than that obtained from adult fibroblasts. We concluded that the nuclear donor cell lines have significant impact on the developmental competence of cloned embryos as well as on the cloning efficiency of Meishan pig.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-14 z 14

      Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies