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    Wyświetlanie 1-8 z 8
    Tytuł:
    Knowledge, protection behaviours and seroprevalence of Lyme borreliosis in inhabitants of Lublin Province, eastern Poland – evaluation of a prophylaxis programme
    Autorzy:
    Zając, V.
    Sroka, J.
    Wójcik-Fatla, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/28763061.pdf
    Data publikacji:
    2023
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2023, 30, 3; 413-424
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Profilaktyka chorób odkleszczowych
    Prevention of tick-borne diseases
    Autorzy:
    Dutkiewicz, J
    Cisak, E.
    Wójcik-Fatla, A.
    Zając, V.
    Sroka, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/179873.pdf
    Data publikacji:
    2014
    Wydawca:
    Centralny Instytut Ochrony Pracy
    Tematy:
    choroby odkleszczowe
    choroby zawodowe
    patogeny
    profilaktyka
    tick-borne diseases
    occupational
    pathogens
    prevention
    Opis:
    Chorobami odkleszczowymi nazywamy choroby zakaźne przenoszone za pośrednictwem drobnych pajęczaków, zwanych kleszczami (lxodida). W Polsce są one wywoływane przez co najmniej 5 gatunków (lub grup gatunków) mikroorganizmów (wirusów, bakterii, pierwotniaków). Spośród nich największe znaczenie ma krętek Borrelia burgdorferi wywołujący boreliozę z Lyme, który jest częstą przyczyną chorób zawodowych u leśników i rolników. Główne kierunki profilaktyki chorób odkleszczowych obejmują: szczepienia ochronne przeciwko kleszczowemu zapaleniu mózgu i opon mózgowo-rdzeniowych, unikanie spożywania surowego mleka, stosowanie odpowiedniej odzieży ochronnej podczas pracy w lesie lub na obrzeżach lasu, przegląd ciała i wzięcie natrysku po powrocie z pracy w lesie, szybkie i umiejętne usuwanie kleszczy przyssanych do ciała, stosowanie repelentów, edukację zdrowotną, opiekę medyczną nad zagrożonymi pracownikami leśnictwa i rolnictwa, działania prewencyjne ze strony pionu bhp lasów państwowych, redukcję liczebności kleszczy w środowisku naturalnym.
    Tick-borne diseases are infectious diseases transmitted by small arachnids called ticks (lxodida). In Poland, these diseases are caused by at least five species (or groups of species) of microorganisms (viruses, bacteria. protozoa). Of which, the most important is the spirochete Borrelia burgdorferi causing Lyme borreliosis, which is the frequent cause of occupational diseases in the forestry and agricultural workers. The main directions of the prevention of tick-borne diseases include: vaccination against tick-borne encephalitis, avoiding of the consumption of raw milk, use of the proper clothing during work in the forest, inspection of the body and taking shower after coming from forest. quick and proper removing of ticks adhered to body, use of repelltents, health education, providing medical care for endangered forestry and agricultural workers, preventive actions pursued by the management of the forest service, reduction of the tick number of natural environment.
    Źródło:
    Bezpieczeństwo Pracy : nauka i praktyka; 2014, 4; 21-23
    0137-7043
    Pojawia się w:
    Bezpieczeństwo Pracy : nauka i praktyka
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Study on Giardia duodenalis and Cryptosporidium spp. infection in veterinarians in Poland
    Autorzy:
    Wójcik-Fatla, A.
    Sroka, J.
    Zając, V.
    Zwoliński, J.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2081928.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Giardia duodenalis
    Cryptosporidium spp.
    veterinarians
    Polska
    Opis:
    The main aim of the study was to determine the risk of two selected zoonosis infections caused by Giardia duodenalis and Cryptosporidium spp. among an occupational group of veterinarians in Poland. Two hundred and ninety-seven samples of stool were tested for the presence of Giardia cysts and Cryptosporidium oocysts using Direct Fluorescent Assay (DFA). There were no positive results for Cryptosporidium. The presence of Giardia cysts was found in two samples of faeces (0.67%). The risk with regard to the parasites Giardia duodenalis and Cryptosporidium spp. seems to be low among the group of veterinarians.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2018, 25, 4; 732-733
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Profilaktyka chorób odzwierzęcych innych niż kleszczowe
    Prevention of zoonotic diseases other than tick-borne
    Autorzy:
    Dutkiewicz, J
    Wójcik-Fatla, A.
    Zając, V.
    Sroka, J.
    Cisak, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/179719.pdf
    Data publikacji:
    2014
    Wydawca:
    Centralny Instytut Ochrony Pracy
    Tematy:
    choroby odzwierzęce
    patogeny
    drogi przenoszenia
    profilaktyka
    zoonoses
    occupational
    pathogens
    transmission routes
    prevention
    Opis:
    W Polsce występuje co najmniej 31 ważnych patogenów (wirusy, bakterie, grzyby, pierwotniaki, robaki), wywołujących zawodowe choroby odzwierzęce inne niż odkleszczowe. Spośród tych patogenów 9 przekazywanych jest głównie drogą powietrzną, 9 - drogą pokarmową i 13 - drogą kontaktu bezpośredniego przez skórę i/lub błony śluzowe. Najważniejsze kierunki profilaktyki chorób odzwierzęcych obejmują: utrzymywanie czystości w pomieszczeniach dla zwierząt i dokonywanie okresowych dezynfekcji, stosowanie odzieży ochronnej i sprzętu ochrony osobistej, profilaktykę medyczną, wybijanie zagrożonych stad, tępienie gryzoni, szczepienia ochronne ludzi i zwierząt, zachowanie szczególnej ostrożności przy odbieraniu porodów u przeżuwaczy, przestrzeganie zasad higieny przy spożywaniu pokarmów i wody i edukację zdrowotną.
    In Poland, there are at least 31 important pathogens (viruses, bacteria, fungi, protozoa, helminths) causing occupational zoonotic diseases other than tick-borne. Of that number, 9 are transmitted mainly by airborne route, 9 by alimentary route and 13 by direct contact through the skin and/or mucous membranes.The main directions using protective clothing and equipment, medical prophylaxis, eradicating endangered flocks, combating rodents, vaccinating humans and animals, taking precautions at parturitions of ruminants, maintaining hygiene at food and water consumption, and health education.
    Źródło:
    Bezpieczeństwo Pracy : nauka i praktyka; 2014, 6; 12-14
    0137-7043
    Pojawia się w:
    Bezpieczeństwo Pracy : nauka i praktyka
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prevalence of Toxoplasma gondii infection in cats in southwestern Poland
    Autorzy:
    Sroka, J.
    Karamon, J.
    Dutkiewicz, J.
    Wójcik-Fatla, A.
    Zając, V.
    Cencek, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/2081746.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Polska
    PCR
    seroprevalence
    Toxoplasma gondii
    IFAT
    cats
    oocysts
    coproscopy
    Opis:
    Objective. An assessment of the prevalence of Toxoplasma gondii infection in cats from southwestern Poland using serology, coproscopy and PCR methods. Materials and method. In total, 208 cats (139 females and 68 males), aged 0.5–12 years (mean=2.6) from 25 localities in southwestern Poland were examined by indirect immunofluorescence assay (IFAT) to estimate the T. gondii serological status. Faecal samples of 41 cats were examined for the presence of oocysts/DNA T. gondii by microscopy and Real-time/nested PCR. After flotation (with NaNO3), pellets from faecal samples were disrupted by 10 cycles of freezing (liquid nitrogen) and warming. DNA was extracted using QIamp DNA Stool Mini Kit (Qiagen), according to the manufacturer’s instruction. Results. The positive results in IFAT for anti-T. gondii IgG and IgM antibodies were found in 143 of 208 tested cats (68.8%). Among positive results, 14.5%, 34.1% and 51.4% were detected in titre ranges 128–512, 1,000–2,000 and ≥ 4,000, respectively. In 23.1% of cat sera anti- T. gondii IgM antibodies were found. The prevalence of anti-Toxoplasma antibodies was significantly greater in older cats (>1 year) (83.5%) than in younger cats (48.3%) (P<0.05), in females (74.1%) than in males (58.8%) (P<0.05), and in cats kept outdoors than indoors (69.7% vs. 16.7%) (P<0.01). Among the 41 faecal samples examined, the presence of structures resembling T. gondii oocysts was found in 2 samples (4.9%), and for one of these samples (2.4% of the total) the result was also confirmed by PCR. Conclusions. T. gondii infection in domestic cats is highly prevalent in southwestern Poland. Information on the prevalence of infection in cats can be useful for assessing T. gondii environmental contamination and the risk for public health.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2018, 25, 3; 1-5
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Streptococcus suis: a re-emerging pathogen associated with occupational exposure to pigs or pork products. Part II – pathogenesis
    Autorzy:
    Dutkiewicz, J.
    Zając, V.
    Sroka, J.
    Wasiński, B.
    Cisak, E.
    Sawczyn, A.
    Kloc, A.
    Wojcik-Fatla, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2081593.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Streptococcus suis
    pathogenesis
    infection stages
    interaction with host cells and tissues
    virulence factors
    genomic
    determinants of pathogenicity
    biofilm formation
    Opis:
    Abstract Streptococcus suis is a re-emerging zoonotic pathogen that may cause severe disease, mostly meningitis, in pigs and in humans having occupational contact with pigs and pork, such as farmers, slaughterhose workers and butchers. The first stage of the pathogenic process, similar in pigs and humans, is adherence to and colonisation of mucosal and/or epithelial surface(s) of the host. The second stage is invasion into deeper tissue and extracellular translocation of bacterium in the bloodstream, either free in circulation or attached to the surface of monocytes. If S. suis present in blood fails to cause fatal septicaemia, it is able to progress into the third stage comprising penetration into host’s organs, mostly by crossing the blood-brain barrier and/or blood–cerebrospinal fluid barrier to gain access to the central nervous system (CNS) and cause meningitis. The fourth stage is inflammation that plays a key role in the pathogenesis of both systemic and CNS infections caused by S. suis. The pathogen may induce the overproduction of pro-inflammatory cytokines that cause septic shock and/or the recruitment and activation of different leukocyte populations, causing acute inflammation of the CNS. Streptococcus suis can also evoke – through activation of microglial cells, astrocytes and possibly other cell types – a fulminant inflammatory reaction of the brain which leads to intracranial complications, including brain oedema, increased intracranial pressure, cerebrovascular insults, and deafness, as a result of cochlear sepsis. In all stages of the pathogenic process, S. suis interacts with many types of immunocompetent host’s cells, such as polymorphonuclear leukocytes, mononuclear macrophages, lymphocytes, dendritic cells and microglia, using a range of versatile virulence factors for evasion of the innate and adaptive immune defence of the host, and for overcoming environmental stress. It is estimated that S. suis produces more than 100 different virulence factors that could be classified into 4 groups: surface components or secreted elements, enzymes, transcription factors or regulatory systems and transporter factors or secretion systems. A major virulence factor is capsular polysaccharide (CPS) that protects bacteria from phagocytosis. However, it hampers adhesion to and invasion of host’s cells, release of inflammatory cytokines and formation of the resistant biofilm which, in many cases, is vital for the persistence of bacteria. It has been demonstrated that the arising by mutation unencapsulated S. suis clones, which are more successful in penetration to and propagation within the host’s cells, may coexist in the organism of a single host together with those that are encapsulated. Both ‘complementary’ clones assist each other in the successful colonization of host’s tissues and persistence therein. S. suis has an open pan-genome characterized by a frequent gene transfer and a large diversity. Of the genetic determinants of S. suis pathogenicity, the most important are pathogenicity islands (PAI), in particular, a novel DNA segment of 89 kb length with evident pathogenic traits that has been designated as 89K PAI. It has been estimated that more than one-third of the S. suis virulence factors is associated with this PAI. It has been proved that the virulent S. suis strains possess smaller genomes, compared to avirulent ones, but more genes associated with virulence. Overall, the evolution of the species most probably aims towards increased pathogenicity, and hence the most significant task of the current research is an elaboration of a vaccine, efficient both for humans and pigs.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2018, 25, 1; 186-203
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Toxoplasma gondii infection in selected species of free-living animals in Poland
    Autorzy:
    Sroka, J.
    Karamon, J.
    Wójcik-Fatla, A.
    Dutkiewicz, J.
    Bilska-Zając, E.
    Zając, V.
    Piotrowska, W.
    Cencek, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085130.pdf
    Data publikacji:
    2019
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Polska
    Toxoplasma gondii
    nested PCR
    genotyping
    free-living animals
    Opis:
    Introduction and objective. Free-living animals can play an important role as a reservoir of Toxoplasma gondi;, however, data concerning this issue in Poland are still limited.The aim of study was to assess the occurrence of T. gondii infection by using molecular methods in free-living animals in selected regions of Poland. Materials and method. Tissues samples of 396 animals (foxes, muskrats, birds, martens, badgers, polecats, raccoons, minks, raccoon dogs, otters, small rodents and insectivores, and grass snakes were collected from various regions of Poland. After samples digestion, DNA was isolated using QIAmp DNA Mini Kit (Qiagen). DNA extraction from small rodents and insectivores samples was performed without digestion. Next, nested PCR (B1 gene) and, for a part of nested PCR positive amplicons, RFLP PCR, were performed according to the method by Grigg and Boothroyd (2001). The other part of nested PCR positive DNA isolates were genotyped using 5 genetic markers: SAG1, SAG2 (5’- and 3’), SAG3, BTUB and GRA6, based on the method by Dubey et al. (2006). These PCR products were sequenced and compared with the NCBI database using Blast. Results. In total, in 50 of the 396 examined animals DNA of T. gondii was detected (12.6%). The highest percentages of positive results in PCR was obtained in martens (40.9%) and badgers (38.5%), lower in birds (27.3%) and the lowest in foxes (7.4%). The RFLP and multilocus PCR analysis showed the dominance of T. gondii clonal type II (or II/III). Conclusions. The results of this study indicate the frequent T. gondii infection among free-living animals in Poland, especially martens and badgers, which may indirectly indicate that these animals contribute to the spread of the parasite in the sylvatic environment in Poland. The genotyping analysis showed the dominance of T. gondii clonal type II (or II/III).
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2019, 26, 4; 656-660
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Potential sources of infection with selected zoonotic agents in the veterinary work environment - pilot studies
    Autorzy:
    Wójcik-Fatla, A.
    Sroka, J.
    Zając, V.
    Sawczyn-Domańska, A.
    Kloc, A.
    Zwoliński, J.
    Kłapeć, T.
    Studzińska, M.B.
    Chmura, R.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085448.pdf
    Data publikacji:
    2020
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    environment
    Coxiella burnetii
    Toxoplasma gondii
    veterinarians
    Leptospira spp
    Cryptosporidium spp
    Giardia duodenalis
    Opis:
    Introduction and Objective. The problem of occupational biohazards is very important, especially in the field of agriculture and in human and veterinary medicine. The aim of the study was to determine the potential sources of infection in veterinary professionals with selected zoonotic agents, including: Toxoplasma gondii, Giardia duodenalis, Leptospira spp., Cryptosporidium spp. and Coxiella burnetii. Materials and Method. A total of 50 air samples from barns, piggeries and veterinary surgeries were examined for the presence of Leptospira spp. and C. burnetii DNA. Serum samples of 86 pigs and 80 cows were tested for the presence of antibodies to Leptospira spp. and to phase I and II C. burnetii antigens. Serum of 70 cats were tested for the presence of antibodies to T. gondii and 65 samples of cat faeces for the presence of T. gondii oocysts. The presence of G. duodenalis and Cryptosporidium spp. were examined in 50 of dog faeces and 50 of bovine faeces samples. Results. DNA of Leptospira spp. was detected in 2 air samples from the piggeries (4%). C. burnetii DNA was not found in any sample. Anti-Leptospira spp. antibodies were detected in 51 (59.3%) of examined pigs. Neither anti-Leptospira spp. nor anti-C. burnetii antibodies were found among samples of bovine serum. Anti-T. gondii antibodies was found in 52 cat serum samples (74.3%). Among samples of cat faeces, no T. gondii oocysts were detected. In one sample of cattle stool (2%), G. duodenalis was detected and in another (2%) – Cryptosporidium spp. G. duodenalis was detected in 7 samples (14%) and Cryptosporidium spp. in 2 samples (2%) of dog faeces. Conclusions. The results of this study demonstrate the potential risk of infection with Leptospira spp. in veterinarians working with pigs. Veterinarians could be also be at risk of infection with T. gondii and G. duodenalis.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2020, 27, 1; 146-150
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-8 z 8

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