- Tytuł:
- The YJL185C, YLR376C and YJR129C genes of Saccharomyces cerevisiae are probably involved in regulation of the glyoxylate cycle
- Autorzy:
-
Boniewska-Bernacka, Ewa
Wysocki, Robert
Grochowalska, Renata
Machnicka, Beata
Ułaszewski, Stanisław
Lachowicz, Tadeusz - Powiązania:
- https://bibliotekanauki.pl/articles/1041166.pdf
- Data publikacji:
- 2006
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
glyoxylate cycle
multicopy suppressor
Saccharomyces cerevisiae
acidification mutants
yeast - Opis:
- The ER24 aci (acidification) mutant of Saccharomyces cerevisiae excreting protons in the absence of glucose was transformed with a multicopy yeast DNA plasmid library. Three different DNA fragments restored the wild-type phenotype termed Aci- because it does not acidify the complete glucose medium under the tested conditions. Molecular dissection of the transforming DNA fragments identified two multicopy suppressor genes YJL185C, YJR129C and one allelic YLR376C. Disruption of either of the three genes in wild-type yeast strain resulted in acidification of the medium (Aci+ phenotype) similarly to the original ER24 mutant. These data indicate the contribution of the ER24 gene product Ylr376Cp and of the two suppressor gene products Yjl185Cp and Yjr129Cp to a complex regulation of the glyoxylate cycle in yeast.
- Źródło:
-
Acta Biochimica Polonica; 2006, 53, 4; 739-745
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł