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    Wyświetlanie 1-7 z 7
    Tytuł:
    The in vitro effect of bovine lactoferrin on the activity of organ leukocytes in rainbow trout [Oncorhynchus mykiss], European eel [Anguilla anguilla] and wels catfish [Silurus glanis]
    Autorzy:
    Malaczewska, J
    Wojcik, M.
    Wojcik, R.
    Siwicki, A.K.
    Powiązania:
    https://bibliotekanauki.pl/articles/30969.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    fish
    rainbow trout
    Oncorhynchus mykiss
    European eel
    Anguilla anguilla
    catfish
    Silurus glanis
    in vitro effect
    bovine lactoferrin
    lactoferrin
    leucocyte
    freshwater fish
    Opis:
    Lactoferrin (LF) is a glycoprotein found in milk, neutrophil granules, secretions and selected organs of mammals. Lactoferrin exhibits antibacterial, antiviral, fungicidal, immunoregulatory and other functions. Although fish are devoid of this protein and its cell receptors, LF effect on the immune mechanisms of fish has been demonstrated. The objective of this study was to investigate the effect of bovine lactoferrin, applied in vitro, on the activity of head kidney and spleen leukocytes in three freshwater fish species: rainbow trout (Oncorhynchus mykiss), European eel (Anguilla anguilla) and weis catfish (Silurus glanis). The obtained results validate LF beneficial effect on the respiratory burst of phagocytes in rainbow trout and weis catfish despite the fact that the potential killing activity against Aeromonas hydrophila was not stimulated in any of the studied species. Bovine lactoferrin enhanced the proliferation of T-lymphocytes in rainbow trout and European eel, as well as of B-lymphocytes in rainbow trout.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 1; 83-88
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The in vitro effect of kynurenic acid on the rainbow trout (Oncorhynchus mykiss) leukocyte and splenocyte activity
    Autorzy:
    Malaczewska, J.
    Siwicki, A.K.
    Wojcik, R.
    Turski, W.A.
    Kaczorek, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/32542.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Kynurenic acid (KYNA), an endogenous neuroprotectant formed along the kynurenine pathway of tryptophan degradation, is a selective ligand of the GPR35 receptor, which can be found on the surface of various populations of human immune cells. In infections and inflammations, KYNA produces an anti-inflammatory effect through this receptor, by depressing the synthesis of reactive oxygen species and pro-inflammatory cytokines. However, it is still unrecognized whether receptors for kynurenic acid are also localized on immune cells of poikilothermic animals, or whether KYNA is able to affect these cells. The objective of this study has been to determine the effect of different concentrations of kynurenic acid (12.5 μM to 10 mM) on the viability and mitogenic response of lymphocytes and on the activity of phagocytic cells isolated from blood and the spleen of rainbow trout. The results imply low toxicity of kynurenic acid towards fish immune cells, and the proliferative effect observed at the two lowest concentrations of KYNA (12.5 μM and 25 μM) seems indicative of endogenous kynurenic acid being capable of activating fish lymphocytes. Non-toxic, micromole concentrations of KYNA, however, had no influence on the mitogenic response of lymphocytes nor on the activity of phagocytes in rainbow trout under in vitro conditions. There is some likelihood that such an effect could be observed at lower, nanomole concentrations of KYNA.
    Źródło:
    Polish Journal of Veterinary Sciences; 2014, 17, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of beta-hydroxy-beta-methylbutyrate (HMB) on the proliferative response of blood lymphocytes and the phagocytic activity of blood monocytes and granulocytes in calves
    Autorzy:
    Wojcik, R.
    Malaczewska, J.
    Siwicki, A.K.
    Micinski, J.
    Zwierzchowski, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/32319.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The objective of this study was to evaluate the effect of HMB on selected indicators of immunity in calves. The experiment was performed on 14 calves aged 30±2 days, divided into two equal groups of control (group I) and experimental (group II) animals. The feed administered to experimental group calves was supplemented with HMB at 40 mg/kg BW, whereas control calves were administered standard farm-made feed without supplementation. Blood was sampled from the jugular vein immediately before the experiment (day 0) and on experimental days 15, 30 and 60 to determine the following parameters of immunity: proliferative response of LPS- and ConA-stimulated lymphocytes (MTT), respiratory burst activity (RBA) and potential killing activity (PKA) of phagocytes. The results revealed a significant increase in RBA and MTT values in calves administered HMB in comparison with the control group throughout the experiment. In the group of animals receiving HMB, an increase in PKA values was noted only on day 30.
    Źródło:
    Polish Journal of Veterinary Sciences; 2013, 16, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of beta-hydroxy-beta-methylbutyrate (HMB) on selected parameters of humoral immunity in calves
    Autorzy:
    Wojcik, R.
    Malaczewska, J.
    Siwicki, A.K.
    Micinski, J.
    Zwierzchowski, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087958.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The objective of this study was to evaluate the effect of HMB on selected parameters of the humoral immunity in calves. The experiment was performed on 14 calves aged 30 ± 2 days, divided into two equal groups of control (group K) and experimental (group H) animals. The feed administered to the experimental calves was supplemented with HMB at 40 mg/kg BW, whereas the control calves were administered standard farm-made feed without supplementation. Blood was sampled from the jugular vein immediately before the experiment (day 0) and on experimental days 15, 30 and 60 to determine the following immunological parameters: total protein levels, gammaglobulin levels, lysozyme activity and ceruloplasmin activity. An analysis of the results obtained revealed a significant increase (p < 0.05; p < 0.01; p < 0.001 respectively) in gammaglobulin levels and lysozyme activity throughout the entire experimental period, an increase (p < 0.05; p < 0.01 respectively) in ceruloplasmin activity on experimental days 15 and 30, but no changes in serum total protein levels of calves administered HMB as compared to those found in the control group.
    Źródło:
    Polish Journal of Veterinary Sciences; 2014, 17, 2; 357-359
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of a diet containing beta-carotene and omega-3 fatty acids on the biochemical and nonspecific humoral immunity indicators and on the results of experimental calf rearing
    Autorzy:
    Zwierzchowski, G.
    Micinski, J.
    Pogorzelska, J.
    Siwicki, A.
    Wojcik, R.
    Kobzhassarov, T.Z.
    Bermagambetova, N.
    Shaikamal, G.I.
    Fijalkowska, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/1189818.pdf
    Data publikacji:
    2016
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
    Tematy:
    animal feeding
    diet
    beta-carotene
    omega-3 fatty acid
    biochemical indicator
    humoral immunity indicator
    immunoglobulin
    calf
    rearing
    Opis:
    The aim of this study has been to determine the effect of a diet containing β-carotene and omega-3 fatty acids on the biochemical and nonspecific humoral immunity indicators and on the results of rearing calves to day 60 of life. The study was conducted in 2011-2012, on a herd of Polish Holstein-Friesian cows. 54 calves from the herd were divided into 3 groups, 18 animals in each: CTR, TRT1 and TRT2. During the colostral period lasting for 5 days after birth, calves were drinking the mother’s colostrum. From 6 to 60 days, calves were watered milk replacer formulation. The control group (CTR) consisted of calves after the colostral period given milk replacer formulation without any supplementation. In the two other groups, calves received milk replacers with the supplementation of b-carotene (TRT1 group) in the amount of 25 mg/calf/day or a suspension of liver omega-3 oil (TRT2 group) in the amount of 5g/calf/day. All calves received solid feed ad libitum. The research showed that the parameters of the metabolic profile of calves (ALT, ASP, CHOL, GLU, UREA, ALP, TG, CRE) were within the normal range, indicating good health of the calves. Moreover, the supplementation had a positive impact on the chosen immunological parameters, including a higher IgG concentration, especially at the end of the milk drinking period. The result of B-carotene in a diet was the highest daily gain in the period from 30 to 60 days of life among the analysed groups of calves. No such effect was achieved in the TRT2 group of calves, as their body weight was the lowest. However, noteworthy is the beneficial impact of beta-carotene and omega-3 oil on the non-specific humoral immunity parameters, which was manifested by fewer cases of clinical diarrhoea and upper respiratory tract diseases.
    Źródło:
    Journal of Elementology; 2016, 21, 1; 283-302
    1644-2296
    Pojawia się w:
    Journal of Elementology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of undecanones and their derivatives on tumor angiogenesis and VEGF content
    Autorzy:
    Gibka, J
    Wasiutynski, A.
    Skopinska-Rozewska, E.
    Siwicki, A.K.
    Chorostowska-Wynimko, J.
    Sommer, E.
    Mazurkiewicz, M.
    Glinski, M.
    Skurzak, H.
    Wojcik, R.
    Jung, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/31734.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    mice
    mouse
    undecan-x-one
    derivative
    angiogenesis
    tumour
    VEGF zob.vascular endothelial growth factor
    enantiomer
    histological examination
    vascular endothelial growth factor
    sarcoma cell
    Opis:
    The in vivo effects of some derivatives of aliphatic ketones (2-undecanone, 3-undecanone, 4-undecanone and their derivatives) on L-l sarcoma tumor angiogenesis and VEGF content were studied in Balb/c mice. Mice that inhaled 10% solution of 3-undecanone(3-on) or 1% solution of 2-undecanone propylene acetal (Acpr2) for 3 days after tumor cells implantation, presented lower neovascular response measured by tumor-induced cutaneous angiogenesis test (TIA) and lower tumor VEGF content in 5-days tumors, than non-inhaled controls. Other substances presented various effects on tumor VEGF concentration and angiogenesis. Histological examination of lesions collected from mice inhaled Acpr2, or non-inhaled controls, revealed small diffused areas of necrosis in the former group. In both groups, slight to moderate inflammatory infiltrations were seen at the tumor's margin. In Acpr2 group, there were less small blood vessels at tumor's margin than in the control group.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 1; 105-115
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of a diet containing beta-carotene and omega-3 fatty acids on the biochemical and nonspecific humoral immunity indicators and on the results of experimental calf rearing
    Autorzy:
    Zwierzchowski, G.
    Micinski, J.
    Pogorzelska, J.
    Siwicki, A.
    Wojcik, R.
    Kobzhassarov, T.Z.
    Bermagambetova, N.
    Shaikamal, G.I.
    Fijalkowska, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/14928.pdf
    Data publikacji:
    2016
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
    Tematy:
    animal feeding
    diet
    beta-carotene
    omega-3 fatty acid
    biochemical indicator
    humoral immunity indicator
    immunoglobulin
    calf
    rearing
    Opis:
    The aim of this study has been to determine the effect of a diet containing β-carotene and omega-3 fatty acids on the biochemical and nonspecific humoral immunity indicators and on the results of rearing calves to day 60 of life. The study was conducted in 2011-2012, on a herd of Polish Holstein-Friesian cows. 54 calves from the herd were divided into 3 groups, 18 animals in each: CTR, TRT1 and TRT2. During the colostral period lasting for 5 days after birth, calves were drinking the mother’s colostrum. From 6 to 60 days, calves were watered milk replacer formulation. The control group (CTR) consisted of calves after the colostral period given milk replacer formulation without any supplementation. In the two other groups, calves received milk replacers with the supplementation of b-carotene (TRT1 group) in the amount of 25 mg/calf/day or a suspension of liver omega-3 oil (TRT2 group) in the amount of 5g/calf/day. All calves received solid feed ad libitum. The research showed that the parameters of the metabolic profile of calves (ALT, ASP, CHOL, GLU, UREA, ALP, TG, CRE) were within the normal range, indicating good health of the calves. Moreover, the supplementation had a positive impact on the chosen immunological parameters, including a higher IgG concentration, especially at the end of the milk drinking period. The result of B-carotene in a diet was the highest daily gain in the period from 30 to 60 days of life among the analysed groups of calves. No such effect was achieved in the TRT2 group of calves, as their body weight was the lowest. However, noteworthy is the beneficial impact of beta-carotene and omega-3 oil on the non-specific humoral immunity parameters, which was manifested by fewer cases of clinical diarrhoea and upper respiratory tract diseases.
    Źródło:
    Journal of Elementology; 2016, 21, 1
    1644-2296
    Pojawia się w:
    Journal of Elementology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-7 z 7

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