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Wyświetlanie 1-4 z 4
Tytuł:
The in vitro effect of bovine lactoferrin on the activity of organ leukocytes in rainbow trout [Oncorhynchus mykiss], European eel [Anguilla anguilla] and wels catfish [Silurus glanis]
Autorzy:
Malaczewska, J
Wojcik, M.
Wojcik, R.
Siwicki, A.K.
Powiązania:
https://bibliotekanauki.pl/articles/30969.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
fish
rainbow trout
Oncorhynchus mykiss
European eel
Anguilla anguilla
catfish
Silurus glanis
in vitro effect
bovine lactoferrin
lactoferrin
leucocyte
freshwater fish
Opis:
Lactoferrin (LF) is a glycoprotein found in milk, neutrophil granules, secretions and selected organs of mammals. Lactoferrin exhibits antibacterial, antiviral, fungicidal, immunoregulatory and other functions. Although fish are devoid of this protein and its cell receptors, LF effect on the immune mechanisms of fish has been demonstrated. The objective of this study was to investigate the effect of bovine lactoferrin, applied in vitro, on the activity of head kidney and spleen leukocytes in three freshwater fish species: rainbow trout (Oncorhynchus mykiss), European eel (Anguilla anguilla) and weis catfish (Silurus glanis). The obtained results validate LF beneficial effect on the respiratory burst of phagocytes in rainbow trout and weis catfish despite the fact that the potential killing activity against Aeromonas hydrophila was not stimulated in any of the studied species. Bovine lactoferrin enhanced the proliferation of T-lymphocytes in rainbow trout and European eel, as well as of B-lymphocytes in rainbow trout.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 1; 83-88
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of beta-hydroxy-beta-methylbutyrate (HMB) on the proliferative response of blood lymphocytes and the phagocytic activity of blood monocytes and granulocytes in calves
Autorzy:
Wojcik, R.
Malaczewska, J.
Siwicki, A.K.
Micinski, J.
Zwierzchowski, G.
Powiązania:
https://bibliotekanauki.pl/articles/32319.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The objective of this study was to evaluate the effect of HMB on selected indicators of immunity in calves. The experiment was performed on 14 calves aged 30±2 days, divided into two equal groups of control (group I) and experimental (group II) animals. The feed administered to experimental group calves was supplemented with HMB at 40 mg/kg BW, whereas control calves were administered standard farm-made feed without supplementation. Blood was sampled from the jugular vein immediately before the experiment (day 0) and on experimental days 15, 30 and 60 to determine the following parameters of immunity: proliferative response of LPS- and ConA-stimulated lymphocytes (MTT), respiratory burst activity (RBA) and potential killing activity (PKA) of phagocytes. The results revealed a significant increase in RBA and MTT values in calves administered HMB in comparison with the control group throughout the experiment. In the group of animals receiving HMB, an increase in PKA values was noted only on day 30.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of beta-hydroxy-beta-methylbutyrate (HMB) on selected parameters of humoral immunity in calves
Autorzy:
Wojcik, R.
Malaczewska, J.
Siwicki, A.K.
Micinski, J.
Zwierzchowski, G.
Powiązania:
https://bibliotekanauki.pl/articles/2087958.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The objective of this study was to evaluate the effect of HMB on selected parameters of the humoral immunity in calves. The experiment was performed on 14 calves aged 30 ± 2 days, divided into two equal groups of control (group K) and experimental (group H) animals. The feed administered to the experimental calves was supplemented with HMB at 40 mg/kg BW, whereas the control calves were administered standard farm-made feed without supplementation. Blood was sampled from the jugular vein immediately before the experiment (day 0) and on experimental days 15, 30 and 60 to determine the following immunological parameters: total protein levels, gammaglobulin levels, lysozyme activity and ceruloplasmin activity. An analysis of the results obtained revealed a significant increase (p < 0.05; p < 0.01; p < 0.001 respectively) in gammaglobulin levels and lysozyme activity throughout the entire experimental period, an increase (p < 0.05; p < 0.01 respectively) in ceruloplasmin activity on experimental days 15 and 30, but no changes in serum total protein levels of calves administered HMB as compared to those found in the control group.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 2; 357-359
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The in vitro effect of kynurenic acid on the rainbow trout (Oncorhynchus mykiss) leukocyte and splenocyte activity
Autorzy:
Malaczewska, J.
Siwicki, A.K.
Wojcik, R.
Turski, W.A.
Kaczorek, E.
Powiązania:
https://bibliotekanauki.pl/articles/32542.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Kynurenic acid (KYNA), an endogenous neuroprotectant formed along the kynurenine pathway of tryptophan degradation, is a selective ligand of the GPR35 receptor, which can be found on the surface of various populations of human immune cells. In infections and inflammations, KYNA produces an anti-inflammatory effect through this receptor, by depressing the synthesis of reactive oxygen species and pro-inflammatory cytokines. However, it is still unrecognized whether receptors for kynurenic acid are also localized on immune cells of poikilothermic animals, or whether KYNA is able to affect these cells. The objective of this study has been to determine the effect of different concentrations of kynurenic acid (12.5 μM to 10 mM) on the viability and mitogenic response of lymphocytes and on the activity of phagocytic cells isolated from blood and the spleen of rainbow trout. The results imply low toxicity of kynurenic acid towards fish immune cells, and the proliferative effect observed at the two lowest concentrations of KYNA (12.5 μM and 25 μM) seems indicative of endogenous kynurenic acid being capable of activating fish lymphocytes. Non-toxic, micromole concentrations of KYNA, however, had no influence on the mitogenic response of lymphocytes nor on the activity of phagocytes in rainbow trout under in vitro conditions. There is some likelihood that such an effect could be observed at lower, nanomole concentrations of KYNA.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-4 z 4

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