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    Tytuł:
    Pentacyclic triterpenoids and polyphenols accumulation in cell suspension culture of Chaenomeles japonica (Thunb.) Lindl. ex Spach
    Autorzy:
    Kikowska, M.
    Wlodarczyk, A.
    Stochmal, A.
    Zuchowski, J.
    Thiem, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/71498.pdf
    Data publikacji:
    2019
    Wydawca:
    Instytut Włókien Naturalnych i Roślin Zielarskich
    Tematy:
    pentacyclic triterpenoid
    triterpenoid
    polyphenol
    accumulation
    cell suspension
    callus
    Chaenomeles japonica
    Japanese quince
    chlorogenic acid
    ursolic acid
    oleanolic acid
    Opis:
    Introduction: Callus and cell suspension cultures are widely applied in investigation of production of highvalue secondary metabolites, which may be used as cosmeceuticals, nutraceuticals and pharmaceuticals. Plant cell cultures are promising alternative to intact plant sources for the production of plant-derived drugs of industrial importance. Objective: The aim of the study was to (i) initiate the cell suspension culture of Chaenomeles japonica from homogenous and uniform callus, (ii) stabilize the selected line and (iii) verify its ability to produce the desired groups of secondary metabolites – pentacyclic triterpenoids and polyphenols. Methods: To establish a cell suspension culture, stabilized and homogeneous callus was selected. Cell cultures were systematically passaged every 2 weeks to fresh liquid medium with the same composition. Biomass from cultures at the growth phase and stationary phase was designated for phytochemical research. UHPLC-DAD-MS analyzes were performed. At the same time, their macroscopic and microscopic observations were carried out. Results: Cells of suspension culture line A2 were characterized by the intense divisions. Cell culture extracts (both from the growth phase and stationary phase) contained pentacyclic triterpenoids. In addition, phenolic compounds (chlorogenic acid and proanthocyanidins type B) and in a small amount also epicatechin are present in the extract of the cells harvested from the growth phase. In the present studies, three pentacyclic triterpenoids were detected and quantified in the extracts of cell suspensions and callus line A2. Ursolic and oleanolic acids were the main triterpenoids in the studied extracts. The cell suspension culture from the growth phase exhibited the highest content of ursolic, oleanolic, and betulinic acid (separately and together). Conclusion: The cell suspension culture of Chaenomeles japonica is a promising source of pentacyclic triterpenoids.
    Źródło:
    Herba Polonica; 2019, 65, 1
    0018-0599
    Pojawia się w:
    Herba Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Evaluation of antiamoebic and antimicrobial activities in vitro of Chaenomeles japonica (Thunb.) Lindl. ex Spach extracts
    Autorzy:
    Klikowska, M.
    Derda, M.
    Thiem, B.
    Wlodarczyk, A.
    Dlugaszewska, J.
    Stochmal, A.
    Zuchowski, J.
    Hadas, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/2117842.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    Acanthamoeba spp.
    chaenomeles japonica
    pentacyclic terpenoids
    plant extracts
    plant
    in vitro cultures
    polyphenols
    staphylococcus aureus
    Opis:
    The free-living Acanthamoeba sp. causes various diseases. Treatment of them is very difficult and not always effective because of encystation, making it highly resistant to antiamoebic drugs. Gram-positive bacteria staphylococcus aureus, Gram-negative bacteria Escherichia coli, and an yeast candida albicans also exhibit outstanding resistance to antimicrobial substances. The search for new natural amoebicidal and antimicrobial agents of plant origin is still of current interest. The aim of the study was to investigate the amoebicidal activity of the extracts obtained from tissue culture and a field-grown plant of chaenomeles japonica against pathogenic trophozoites of Acanthamoeba spp. and antimicrobial effect against s. aureus, E. coli, and c. albicans. The extracts of c. japonica had an inhibitory effect on the proliferation of Acanthamoeba trophozoites as compared to the non-treated control. among the crude extracts tested, the extract of leaves, from both shoot culture and the field-grown plant had remarkable amoebicidal action against the trophozoites but also antibacterial activity against Gram-positive bacteria staphylococcus aureus. The extract from leaves from shoot culture, already on the second and third days of treatment, showed an antiamoebicidal effect at a concentration of 1 mg ml-1 (inhibi- tion of trophozoites 87.5% and 91.8%, respectively). In addition to leaves from shoot culture (a conc. 5 mg ml-1, 2nd day inhibition of trophozoites 85.7% and 3rd day 97.2%), leaves from a field-grown plant (a conc. 5 mg ml-1, 2nd day 91.0% and 3rd day 94.4%) and callus (a conc. 5 mg ml-1, 2nd day 90.0% and 3rd day – 95.4%) also exhib- ited a good antiamoebicidal activity. Out of the four extracts, the extracts from leaves from both shoot culture and a field-grown plant were reported to be the most active against Gram-positive s. aureus, which was determined by the values of MIC = 5.0 mg ml-1 and MIC = 2.5 mg ml-1, respectively. The inhibitory potential depends on the yield and composition of mainly bioactive compounds: pentacyclic terpenoids (mainly betulinic, ursolic, and oleanolic acids) and polyphenols (mainly chlorogenic acid and its isomers, epicatechin, dimeric, and trimeric proanthocyanidins, quercetin and kaempferol derivatives).
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2019, 61, 2; 47-58
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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