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Wyświetlanie 1-3 z 3
Tytuł:
Simultaneous measurement of refractive index and thickness for optically transparent object with a dual-wavelength quantitative technique
Autorzy:
Xu, X.
Wang, Y.
Xu, Y.
Jin, W.
Powiązania:
https://bibliotekanauki.pl/articles/174666.pdf
Data publikacji:
2016
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
digital holography
phase measurement
cell measurement
red blood cell
Opis:
We present a new dual-wavelength quantitative measurement approach that can be employed for simultaneously measuring both the refractive index and the thickness of the homogenous specimen. This method is realized by dual-wavelength in-line phase-shifting digital holography, and then the phase images are obtained by using four-phase step algorithm for each wavelength separately. Based on computer simulation technology, the feasibility and the effectiveness of our proposed method are demonstrated by comparing our simulation results with the experimental results of the spherical silica bead and the red blood cell, respectively. Our work will provide some guidance in the experimental research for transparent phase objects.
Źródło:
Optica Applicata; 2016, 46, 4; 597-605
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phase features of several typical blood cells and their identification without unwrapping
Autorzy:
Wang, Y.
Chen, Y
Lü, C.
Shang, X.
Xu, Y.
Wu, H.
Zhu, X.
Jin, W.
Powiązania:
https://bibliotekanauki.pl/articles/173588.pdf
Data publikacji:
2013
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
digital holographic phase
nucleated cell
phase model
wrapped phase features
identification
Opis:
The digital holographic phase microscopy (DHPM) technique which has been proposed for cellular morphology and dynamic analysis yielded highly desirable results. However, for nucleated cells (especially white blood cells (WBCs)), their submicroscopic structure has not yet been deconstructed through a phase unwrapping method due to the heterogeneity of an internal phase. By analyzing the phase heterogeneity of subclasses of WBCs, the typical phase models of them are built first in this paper; using the simulation method, the wrapped phase distributions of these models are obtained. However, by optimizing the wrapped phase maps and analyzing the relationships between them and typical blood cells, their features are selected and extracted. Then the models built are sorted out from each other successfully without unwrapping via analyzing these extracted features, which provides a valuable approach and technological base for the classification and identification of blood cells.
Źródło:
Optica Applicata; 2013, 43, 3; 505-514
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
GP5 protein - based ELISA for the detection of PRRSV antibodies
Autorzy:
Wang, Y.
Guo, J.
Qiuao, S.
Li, Q.
Yang, J.
Jin, Q.
Zhang, G.
Powiązania:
https://bibliotekanauki.pl/articles/30629.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Porcine reproductive and respiratory syndrome virus (PRRSV) is an important swine pathogen, causing huge economic losses each year worldwide. Immunization with vaccines containing the glycoprotein 5 (GP5) of PRRSV is the main measure to induce neutralizing antibodies and control the disease. Here, we developed a GP5 protein-based ELISA for detecting antibodies against PRRSV. The overall yield of purified GP5 in E. coli flask culture was more than 45 mg/L cell culture. Western blot and IFA indicated that the GP5 protein was highly immunogenic. After optimization and validation with IDEXX PRRS using 566 clinical sera, the DSN, DSP, and accuracy of GP5-ELISA were 81.39%, 75.96%, and 80.39%, respectively. Besides, GP5-ELISA is highly specific, showing no cross-reactions with sera against other important swine pathogens. Hence, GP5 is a good diagnostic antigen and the GP5 protein-based ELISA has the potential to be used in the field
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-3 z 3

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