- Tytuł:
-
Badania immunobiochemiczne surowic białych szczurów zarażonych doświadczalnie Toxoplasma gondii
Studies on immunobiochemical sera of white rats with experimental Toxoplasma gondii infection
Immunobiokhimicheskie issledovanija syvorotok belykh krys ehksperimenyalno inficirobannykh pri pomoshhi Toxoplasma gondii - Autorzy:
-
Cegielka, M.
Uminski, J.
Tos-Luty, S. - Powiązania:
- https://bibliotekanauki.pl/articles/838175.pdf
- Data publikacji:
- 1964
- Wydawca:
- Polskie Towarzystwo Parazytologiczne
- Opis:
- White rats (10) were infected with RH Toxoplasma gondii strain administering a Toxoplasma suspension on 7 occasions at 3-day intervals (2 ml of the peritoneal exudate from infected mice, at the dilution of 1:10). The obtained rat sera gave a positive reaction in the complement fixation test with Toxoplasma antigen (1:10-1:160). The electroproteinograms of immune sera (paper electrophoresis, staining according to Grassman-Hannig, photodensitometric measurements) were found to contain albumins and globulins α₁ and α₂, β, γ; as compared with the sera from non-infected rats there were hyperproteinemia and increase in β- and γ- globulins. The inactivation of sera at 56° did not affect the electroproteinogram pattern. Similar results were obtained by electrophoresis of immune sera on gel agar, with the difference that the γ-globulin fraction was extended to γ₁ and γ₂ subfractions. Immunoelectrophoresis (according to Grabar-Williams' and Scheideger's methods) of immune sera, as compared with those of non-infected rat sera, showed more conspicuous precipitation lines in the zones α₂, β and γ as well as a more clear-cut line corresponding to β₂M subfraction. The inactivated sera: gave a smaller number of precipitation lines. Preliminary studies by microdiffusion and agar gel techniques revealed the presence of 5 precipitation lines in the immune sera and only 3 lines in the normal ones. The inactivation of sera diminished the number of precipitation lines to 2 in immune sera, and to 1 in those of non-infected rats. Quantitative determinations of the fractions are under way.
- Źródło:
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Annals of Parasitology; 1964, 10, 4-5
0043-5163 - Pojawia się w:
- Annals of Parasitology
- Dostawca treści:
- Biblioteka Nauki