Autor: Strzelczyk, Joanna. - Katalog OPAC zbiorów

Tytuł:: Expression profiles of MGMT, p16, and APC genes in tumor and matching surgical margin from patients with oral squamous cell carcinoma
Autorzy:: Strzelczyk, Joanna
Gołąbek, Karolina
Krakowczyk, Łukasz
Owczarek, Aleksander
Powiązania:: https://bibliotekanauki.pl/articles/1038771.pdf
Data publikacji:: 2016
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: HNSCC
gene expression
surgical margin
MGMT
p16
APC
Opis:: The aim of the study was to assess the expression of MGMT, p16, and APC genes in tumors and matching surgical margin samples from 56 patients with primary OSCC. We also analyzed the association of the clinical variables with the expression of the studied genes. After RNA isolation and cDNA synthesis gene expression levels were assessed by quantitative reverse transcription (qRT)-PCR. Two-sided parametrical Student's t-test for independent groups with equal/unequal variances showed no statistically significant differences in genes' expression in tumor compared to margin samples. No association was found between the genes' expression and clinical parameters, except for MGMT, whose low expression was probably associated with smoking (0.87 vs 1.34, p=0.065). 'Field cancerization' is an area with genetically or epigenetically altered cells and at the same time a risk factor for cancer. Disturbances in gene expression could also be the source of damages leading to cancerization. In conclusion, it is important to mention that the field remaining after a surgery may pose an increased risk of cancer development. It may be suggested that the diagnosis and treatment of cancers should not be concentrated only on the tumor itself, but also on the cancer field effect. Therefore, further molecular analysis on surgical margins and additional research regarding their assessment are required.
Źródło:: Acta Biochimica Polonica; 2016, 63, 3; 505-509
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 13.

Tytuł:: Nucleotide substitutions in the Candida albicans ERG11 gene of azole-susceptible and azole-resistant clinical isolates
Autorzy:: Strzelczyk, Joanna
Ślemp-Migiel, Anna
Rother, Magdalena
Gołąbek, Karolina
Wiczkowski, Andrzej
Powiązania:: https://bibliotekanauki.pl/articles/1039442.pdf
Data publikacji:: 2013
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: Candida albicans
ERG11 gene
sterol 14α demethylase
azole resistance
Opis:: One of the mechanisms of Candida albicans resistance to azole drugs used in antifungal therapy relies on increased expression and presence of point mutations in the ERG11 gene that encodes sterol 14α demethylase (14DM), an enzyme which is the primary target for the azole class of antifungals. The aim of the study was to analyze nucleotide substitutions in the Candida albicans ERG11 gene of azole-susceptible and azole-resistant clinical isolates. The Candida albicans isolates represented a collection of 122 strains selected from 658 strains isolated from different biological materials. Samples were obtained from hospitalized patients. Fluconazole susceptibility was tested in vitro using a microdilution assay. Candida albicans strains used in this study consisted of two groups: 61 of the isolates were susceptible to azoles and the 61 were resistant to azoles. Four overlapping regions of the ERG11 gene of the isolates of Candida albicans strains were amplified and sequenced. The MSSCP (multitemperature single strand conformation polymorphism) method was performed to select Candida albicans samples presenting genetic differences in the ERG11 gene fragments for subsequent sequence analysis. Based on the sequencing results we managed to detect 19 substitutions of nucleotides in the ERG11 gene fragments. Sequencing revealed 4 different alterations: T495A, A530C, G622A and A945C leading to changes in the corresponding amino acid sequence: D116E, K128T, V159I and E266D. The single nucleotide changes in the ERG11 gene did not affect the sensitivity of Candida albicans strains, whereas multiple nucleotide substitutions in the ERG11 gene fragments indicated a possible relation with the increase in resistance to azole drugs.
Źródło:: Acta Biochimica Polonica; 2013, 60, 4; 547-552
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 14.

Tytuł:: Selected mechanisms of molecular resistance of Candida albicans to azole drugs
Autorzy:: Gołąbek, Karolina
Strzelczyk, Joanna
Owczarek, Aleksander
Cuber, Piotr
Ślemp-Migiel, Anna
Wiczkowski, Andrzej
Powiązania:: https://bibliotekanauki.pl/articles/1039099.pdf
Data publikacji:: 2015
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: Candida albicans
drug resistance
azoles
Opis:: A phenomenon of increasing resistance of Candida spp. to azoles has been observed for several years now. One of the mechanisms of lack of sensitivity to azoles is associated with CDR1, CDR2, MRD1 genes (their products are active transport pumps conditioning drug efflux from pathogen's cell), and ERG11 gene (encoding lanosterol 14α-demethylase). Test material was 120 strains of Candida albicans (60 resistant and 60 susceptible to azole drugs) obtained from clinical samples. The first stage of experiment assessed the expression of CDR1, CDR2, MDR1 and ERG11 genes by Q-PCR. The impact of ERG11 gene's mutations on the expression of this gene was analysed. The final stage of the experiment assessed the level of genome methylation of Candida albicans strains. An increase in the expression of CDR2, MDR1 and ERG11 was observed in azole-resistant strains of Candida albicans in comparison to strains sensitive to this class of drugs. Furthermore, 19 changes in the sequence of ERG11 were detected in tested strains. Four of the discovered mutations: T495A, A530C, G622A and A945C led to the following amino acid substitutions: D116E, K128T, V159I and E266D, respectively. It has also been found that statistically five mutations: T462C, G1309A, C216T, C1257T and A945C affected the expression of ERG11. The applied method of assessing the level of methylation of Candida albicans genome did not confirm its role in the development of resistance to azoles. The results indicate however, that resistance of Candida albicans strains to azole drugs is multifactorial.
Źródło:: Acta Biochimica Polonica; 2015, 62, 2; 247-251
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 15.

Tytuł:: The role of activation of insulin-like growth factors in colorectal cancer
Autorzy:: Walkiewicz, Katarzyna
Nowakowska-Zajdel, Ewa
Strzelczyk, Joanna
Walczak, Michał
Copija, Angelika
Janion, Karolina
Muc-Wierzgoń, Małgorzata
Powiązania:: https://bibliotekanauki.pl/articles/1062926.pdf
Data publikacji:: 2017
Wydawca:: Medical Education
Tematy:: IGF-receptors
colorectal cancer
insulin-like growth factors
metabolic disorders
Opis:: Co-occurrence of metabolic disorders is a recognized risk factor for the development of colorectal cancer which is currently the leading cause of morbidity due to malignant neoplasms in the world. The pathogenesis of colorectal cancer is not well understood yet. Among the postulated neoplastic mechanisms is the activation of insulin-like growth factors, with both epidemiological and clinical observations of their role. In this paper, the authors synthesize the current knowledge about the importance of activation of insulin-like growth factors in the development of colorectal cancer.
Źródło:: OncoReview; 2017, 7, 3; 116-120
2450-6125
Pojawia się w:: OncoReview
Dostawca treści:: Biblioteka Nauki

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