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    Wyświetlanie 1-6 z 6
    Tytuł:
    Host Range of Cyclospora Species: Zoonotic Implication
    Autorzy:
    Solarczyk, Piotr
    Powiązania:
    https://bibliotekanauki.pl/articles/2041956.pdf
    Data publikacji:
    2021
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Cyclospora
    animals
    zoonosis
    epidemiology
    genotyping
    next-generation sequencing
    Opis:
    Cyclospora is an intracellular, gastrointestinal parasite found in birds and mammals worldwide. Limited accessibility of the protozoan for experimental use, scarcity, genome heterogeneity of the isolates and narrow panel of molecular markers hamper zoonotic investigations. One of the significant limitation in zoonotic studies is the lack of precise molecular tools that would be useful in linking animal vectors as a source of human infection. Strong and convincing evidence of zoonotic features will be achieved through proper typing of Cyclospora spp. taxonomic units (e.g. species or genotypes) in animal reservoirs. The most promising method that can be employ for zoonotic surveys is next-generation sequencing.
    Źródło:
    Acta Protozoologica; 2021, 60; 13-20
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    First record of Giardia assemblage D infection in farmed raccoon dogs (Nyctereutes procyonoides)
    Autorzy:
    Solarczyk, Piotr
    Majewska, Anna C.
    Jędrzejewski, Szymon
    Górecki, Marcin T.
    Nowicki, Sławomir
    Przysiecki, Piotr
    Powiązania:
    https://bibliotekanauki.pl/articles/989740.pdf
    Data publikacji:
    2016
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    giardia
    molecular genotyping
    raccoon dog
    Opis:
    The presence of Giardia genotypes was investigated in 18 raccoon dogs (Nyctereutes procyonoides) and 80 red foxes (Vulpes vulpes) on one farm. To demonstrate Giardia cysts, fresh and trichrome stained smears were microscopically screened. Two molecular markers were used for Giardia genotyping: a fragment of the beta-giardin gene and a fragment of the glutamate dehydrogenase gene. All faecal samples obtained from red foxes were negative. Giardia cysts were identified only in 2 of the 18 raccoon dogs. The result of genotyping and phylogenetic analysis showed that the G. duodenalis from both raccoon dogs belonged to the D assemblage. This finding of a new animal reservoir of G. duodenalis canids-specific genotypes is important in order to eliminate the risk of infecting other animals bred for fur. Further molecular analyses of Giardia isolates in raccoon dogs are required. The present study represents the first contribution to knowledge of G. duodenalis genotypes in raccoon dogs.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2016, 23, 4
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    First Molecular Detection of Giardia duodenalis Assemblage B in a Free-Living European Wildcat (Felis s. silvestris) from Luxembourg
    Autorzy:
    Solarczyk, Piotr
    Osten-Sacken, Natalia
    Frantz, Alain C.
    Schneider, Simone
    Pir, Jacques B.
    Heddergott, Mike
    Powiązania:
    https://bibliotekanauki.pl/articles/52071478.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Giardia
    molecular diagnosis
    LAMP
    PCR
    epidemiology
    zoonosis
    Opis:
    Giardia duodenalis is one of the most widespread intestinal parasites of humans and other vertebrates. In terms of public health, identification of Giardia assemblages in wildlife is important because only some assemblages of G. duodenalis can infect humans. Here, we use loop-mediated isothermal amplification (LAMP) and genotyping of analysis of the β-giardin gene to screen the zoonotic assemblages of G. duodenalis recovered from faeces of free-living European wildcats (Felis s. silvestris) from Luxembourg. Giardia DNA was detected in one animal (10%) and assigned to assemblage B by both methods. This is the first detection and genotyping of G. duodenalis in a European wild felid in general, and of assemblage B in particular. Free-living wildcats may act as reservoirs of G. duodenalis infectious for humans and other wildlife and domestic animals. Using a combination of LAMP- and genotyping-based methods allowed effective, sensitive, and rapid detection of a zoonotic G. duodenalis assemblage B in wildlife.
    Źródło:
    Acta Protozoologica; 2019, 58, 1; 1-5
    0065-1583
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    New Primers for Fast Detection of Giardia duodenalis Assemblages A and B Using Real-time PCR
    Autorzy:
    Solarczyk, Piotr
    Wojtkowiak-Giera, Agnieszka
    Hołysz, Marcin
    Słodkowicz-Kowalska, Anna
    Jagodziński, Paweł P.
    Stojecki, Krzysztof
    Rocka, Anna
    Majewska, Anna C.
    Skrzypczak, Łukasz
    Powiązania:
    https://bibliotekanauki.pl/articles/52160005.pdf
    Data publikacji:
    2018
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Giardia duodenalis
    real-time PCR
    specific primers
    genotyping
    zoonoses
    Opis:
    Giardia duodenalisis one of the six Giardia species and itis the most common, cosmopolitan flagellate that infects humans and many species of animals. This species exhibits considerable genetic diversity; to date, eight assemblages (A–H) have been defined. These assemblages differ in host specificity: assemblages A and B have been found in both humans and in many animal species. Mixed infections with Giardia (A and B) assemblages have been reported in humans and in animals. Many molecular techniques are effective and rapid for the detection of G. duodenalis and also for the determination of genetic variability of isolates in clinical and environmental samples. In this context, the aim of this study was to design new assemblage-specific primers for rapid detection and identification of G. duodenalis assemblages A and B and both of these assemblages simultaneously using quantitative real-time polymerase chain reaction (qPCR). Fragments of glutamate dehydrogenase and triose phosphate isomerase were used as targets in the design of primers. In conclusion, the use of G. duodenalis assemblage-specific primers designed in this study allows quick identification of human infectious G. duodenalis assemblages A and B as well as mixed AB assemblages in a sample without further sequencing of the amplification products, which reduces the cost of study and the waiting time for the results.
    Źródło:
    Acta Protozoologica; 2018, 57, 1; 43-48
    0065-1583
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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