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Wyszukujesz frazę "Majewska, Anna C." wg kryterium: Autor


Wyświetlanie 1-2 z 2
Tytuł:
First record of Giardia assemblage D infection in farmed raccoon dogs (Nyctereutes procyonoides)
Autorzy:
Solarczyk, Piotr
Majewska, Anna C.
Jędrzejewski, Szymon
Górecki, Marcin T.
Nowicki, Sławomir
Przysiecki, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/989740.pdf
Data publikacji:
2016
Wydawca:
Instytut Medycyny Wsi
Tematy:
giardia
molecular genotyping
raccoon dog
Opis:
The presence of Giardia genotypes was investigated in 18 raccoon dogs (Nyctereutes procyonoides) and 80 red foxes (Vulpes vulpes) on one farm. To demonstrate Giardia cysts, fresh and trichrome stained smears were microscopically screened. Two molecular markers were used for Giardia genotyping: a fragment of the beta-giardin gene and a fragment of the glutamate dehydrogenase gene. All faecal samples obtained from red foxes were negative. Giardia cysts were identified only in 2 of the 18 raccoon dogs. The result of genotyping and phylogenetic analysis showed that the G. duodenalis from both raccoon dogs belonged to the D assemblage. This finding of a new animal reservoir of G. duodenalis canids-specific genotypes is important in order to eliminate the risk of infecting other animals bred for fur. Further molecular analyses of Giardia isolates in raccoon dogs are required. The present study represents the first contribution to knowledge of G. duodenalis genotypes in raccoon dogs.
Źródło:
Annals of Agricultural and Environmental Medicine; 2016, 23, 4
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
New Primers for Fast Detection of Giardia duodenalis Assemblages A and B Using Real-time PCR
Autorzy:
Solarczyk, Piotr
Wojtkowiak-Giera, Agnieszka
Hołysz, Marcin
Słodkowicz-Kowalska, Anna
Jagodziński, Paweł P.
Stojecki, Krzysztof
Rocka, Anna
Majewska, Anna C.
Skrzypczak, Łukasz
Powiązania:
https://bibliotekanauki.pl/articles/52160005.pdf
Data publikacji:
2018
Wydawca:
Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
Tematy:
Giardia duodenalis
real-time PCR
specific primers
genotyping
zoonoses
Opis:
Giardia duodenalisis one of the six Giardia species and itis the most common, cosmopolitan flagellate that infects humans and many species of animals. This species exhibits considerable genetic diversity; to date, eight assemblages (A–H) have been defined. These assemblages differ in host specificity: assemblages A and B have been found in both humans and in many animal species. Mixed infections with Giardia (A and B) assemblages have been reported in humans and in animals. Many molecular techniques are effective and rapid for the detection of G. duodenalis and also for the determination of genetic variability of isolates in clinical and environmental samples. In this context, the aim of this study was to design new assemblage-specific primers for rapid detection and identification of G. duodenalis assemblages A and B and both of these assemblages simultaneously using quantitative real-time polymerase chain reaction (qPCR). Fragments of glutamate dehydrogenase and triose phosphate isomerase were used as targets in the design of primers. In conclusion, the use of G. duodenalis assemblage-specific primers designed in this study allows quick identification of human infectious G. duodenalis assemblages A and B as well as mixed AB assemblages in a sample without further sequencing of the amplification products, which reduces the cost of study and the waiting time for the results.
Źródło:
Acta Protozoologica; 2018, 57, 1; 43-48
0065-1583
1689-0027
Pojawia się w:
Acta Protozoologica
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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