- Tytuł:
- Desorption/ionization on silicon for small molecules: a promising alternative to MALDI TOF.
- Autorzy:
-
Kraj, Agnieszka
Dylag, Tomasz
Gorecka-Drzazga, Anna
Bargiel, Sylwester
Dziuban, Jan
Silberring, Jerzy - Powiązania:
- https://bibliotekanauki.pl/articles/1043452.pdf
- Data publikacji:
- 2003
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
proteomics
porous silicon
catecholamines
peptides
DIOS
mass spectrometry
desorption/ionization
MALDI TOF - Opis:
- A method has been developed for laser desorption/ionization of catecholamines from porous silicon. This methodology is particularly attractive for analysis of small molecules. MALDI TOF mass spectrometry, although a very sensitive technique, utilizes matrices that need to be mixed with the sample prior to their analysis. Each matrix produces its own background, particularly in the low-molecular mass region. Therefore, detection and identification of molecules below 400 Da can be difficult. Desorption/ionization of samples deposited on porous silicon does not require addition of a matrix, thus, spectra in the low-molecular mass region can be clearly readable. Here, we describe a method for the analysis of catecholamines. While MALDI TOF is superior for proteomics/peptidomics, desorption/ionization from porous silicon can extend the operating range of a mass spectrometer for studies on metabolomics (small organic molecules and their metabolites, such as chemical neurotransmitters, prostaglandins, steroids, etc.).
- Źródło:
-
Acta Biochimica Polonica; 2003, 50, 3; 783-787
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł