- Tytuł:
- Protective role of L-methionine against free radical damage of rat brain synaptosomes
- Autorzy:
-
Slyshenkov, Vyacheslav
Shevalye, Anna
Liopo, Anton
Wojtczak, Lech - Powiązania:
- https://bibliotekanauki.pl/articles/1043695.pdf
- Data publikacji:
- 2002
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
(Na+,K+)-ATPase
oxygen free radicals
brain
synaptosomes
tert-butylhydroperoxide
L-methionine - Opis:
- Incubation of rat brain synaptosomal/mitochondrial fraction with tert-butylhydroperoxide resulted in accumulation of the lipid peroxidation product, conjugated dienes, damage of the synaptosomal membrane as evidenced by leakage of lactate dehydrogenase, and decrease of the total content of glutathione and of the GSH/GSSG ratio. This treatment also produced a considerable decrease of the ouabain-sensitive ATPase activity and a much smaller diminution of the activities of glutathione reductase and glutathione transferase. Preincubation of the synaptosomal/mitochondrial fraction with 0.5 or 1.0 mM L-methionine significantly protected against lipid peroxidation, membrane damage and changes in the glutathione system produced by low (1 mM) concentrations of tert-butylhydroperoxide and completely prevented inactivation of ouabain-sensitive ATPase, glutathione reductase and glutathione transferase by such treatment. The importance of L-methionine in antioxidant protection is discussed.
- Źródło:
-
Acta Biochimica Polonica; 2002, 49, 4; 907-916
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł