- Tytuł:
- Mapping of a transcription promoter located inside the priA gene of the Bacillus subtilis chromosome
- Autorzy:
-
Hinc, Krzysztof
Iwanicki, Adam
Seror, Simone
Obuchowski, Michał - Powiązania:
- https://bibliotekanauki.pl/articles/1041205.pdf
- Data publikacji:
- 2006
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
weak promoters
Bacillus subtilis
transcription - Opis:
- The genome sequence of the Gram-positive soil bacterium Bacillus subtilis was completed in 1997 (Kunst et al., 1998) and the results included the identification of a putative transcription unit encompassing the yloI to yloS genes. Within this region of the B. subtilis chromosome 11 putative open reading frames were found with a wide diversity of probable functions. In this work we have analyzed transcription in the region of the priA-cpgA genes and we have mapped a promoter which is located inside the priA gene and its activity directs transcription of the def-yloM genes. Moreover, this transcript can be extended at low level to the prpC-priK-cpgA genes. Analysis of the sequence in proximity of the transcription start site revealed a sequence suitable for the housekeeping σA subunit of RNA polymerase. Analysis of the β-glactosidase activity of transcription fusions revealed that the identified promoter is active at low level and its activity is increased during late exponential phase of growth.
- Źródło:
-
Acta Biochimica Polonica; 2006, 53, 3; 497-505
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł