- Tytuł:
- A simple way to overcome the recalcitrance of the water fern Ceratopteris thalictroides (L.) Brongn. to cryopreservation
- Autorzy:
-
Makowski, D.
Rybczynski, J.J.
Mikula, A. - Powiązania:
- https://bibliotekanauki.pl/articles/57732.pdf
- Data publikacji:
- 2015
- Wydawca:
- Polskie Towarzystwo Botaniczne
- Tematy:
-
abscisic acid
water fern
fern
Ceratopteris thalictroides
cryopreservation
encapsulation-dehydration method
darkness - Opis:
- Ceratopteris thalictroides is a water fern very sensitive to both dehydration and low temperature. This study focuses on the cryopreservation of this species by encapsulation-dehydration technique, in particular on the effects of pre-culture step, alginate bead size and the physical conditions of culture on the cryopreservation efficiency. Encapsulated and non-pre-cultured gametophytes did not survive cooling with liquid nitrogen. When cryopreservation was preceded by a 2-week period of pre-culture, regrowth reached 42.1%. Reduction in the size of the alginate bead, and culture in total darkness resulted in improved gametophyte regrowth capacity (75.5% or 81.7%, respectively). The best results (91.3%) were obtained when all factors tested occurred simultaneously. The gametophytes recovered very quickly and sporophytes were formed within 4 weeks after rewarming. These simple improvements can be used, not only for the cryopreservation of gametophytes in cryptogams but also for some recalcitrant species of seed plants.
- Źródło:
-
Acta Societatis Botanicorum Poloniae; 2015, 84, 3
0001-6977
2083-9480 - Pojawia się w:
- Acta Societatis Botanicorum Poloniae
- Dostawca treści:
- Biblioteka Nauki
Artykuł