- Tytuł:
- Supramolecularity creates nonstandard protein ligands
- Autorzy:
-
Piekarska, Barbara
Rybarska, Janina
Stopa, Barbara
Zemanek, Grzegorz
Król, Marcin
Roterman, Irena
Konieczny, Leszek - Powiązania:
- https://bibliotekanauki.pl/articles/1044440.pdf
- Data publikacji:
- 1999
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
immunological signal
supramolecularity
Congo red
IgG - Opis:
- Congo red and a group of structurally related dyes long used to stain amyloid proteins are known to associate in water solutions. The self-association of some dyes belonging to this group appears particularly strong. In water solutions their molecules are arranged in ribbon-like micellar forms with liquid crystalline properties. These compounds have recently been found to form complexeswith some native proteins in a non-standard way. Gaps formed by the local distribution of β-sheets in proteins probably represent the receptor sites for these dye ligands. They may result from higher structural instability in unfolding conditions, but also may appear as long range cooperative fluctuations generated by ligand binding. Immunoglobulins G were chosen as model binding proteins to check the mechanism of binding of these dyes. The sites of structural changes generated by antigen binding in antibodies, believed to act as a signal propagated to distant parts of the molecule, were assumed to be suitable sites for the complexation of liquid-crystalline dyes. This assumption was confirmed by proving that antibodies engaged in immune complexation really do bind these dyes; as expected, this binding affects their function by significantly enhancing antigen binding and simultaneously inhibiting C1q attachment. Binding of these supramolecular dyes by some other native proteins including serpins and their natural complexes was also shown. The strict dependence of the ligation properties on strong self-assembling and the particular arrangement of dye molecules indicate that supramolecularity is the feature that creates non-standard protein ligands, with potential uses in medicine and experimental science.
- Źródło:
-
Acta Biochimica Polonica; 1999, 46, 4; 841-851
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł