- Tytuł:
- Molecular cloning and expression analysis of a new gene for short-chain dehydrogenase/reductase 9
- Autorzy:
-
Liu, Shen
Huang, Chaoqun
Li, Dawei
Ren, Weihua
Zhang, Haoxing
Qi, Meiyan
Li, Xin
Yu, Long - Powiązania:
- https://bibliotekanauki.pl/articles/1041143.pdf
- Data publikacji:
- 2007
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
liver
clone
short-chain dehydrogenase/reductase - Opis:
- We report here the cloning and characterization of a novel human short-chain dehydrogenases/reductase gene SCDR9, isolated from a human liver cDNA library, and mapped to 4q22.1 by browsing the UCSC genomic database. SCDR9 containing an ORF with a length of 900 bp, encoding a protein with a signal peptide sequence and an adh_short domain. GFP localization shows SCDR9 protein concentrated in some site of the cytoplasm, but not in the ER. Expression pattern in eighteen tissues revealed that SCDR9 is expressed highly in liver. Soluble recombinant protein was successfully purified from Escherichia coli using pET28A(+) expression vector. Our data provides important information for further study of the function of the SCDR9 gene and its products.
- Źródło:
-
Acta Biochimica Polonica; 2007, 54, 1; 213-218
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł