- Tytuł:
- The nuclear cap-binding protein complex is not essential for nonsense-mediated mRNA decay (NMD) in plants
- Autorzy:
-
Dzikiewicz-Krawczyk, Agnieszka
Piontek, Paulina
Szweykowska-Kulińska, Zofia
Jarmołowski, Artur - Powiązania:
- https://bibliotekanauki.pl/articles/1040693.pdf
- Data publikacji:
- 2008
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
nuclear cap-binding protein complex
alternative splicing
mRNA surveillance
NMD
premature terminatin codon
nonsense-mediated mRNA decay - Opis:
- In this study we investigated whether in plants, like in mammals, components of the nuclear cap-binding protein complex (CBC) are involved in nonsense-mediated mRNA decay (NMD). We selected several genes producing at least two alternatively spliced mRNA variants: one with a premature termination codon (PTC+) and another without it (PTC-). For each gene the PTC+/PTC- ratio was calculated using RT-PCR and direct sequencing in four Arabidopsis thaliana lines: wild type, the NMD mutant atupf3-1 and two CBC mutants: cbp20 and abh1. Whereas in the NMD mutant the ratios of PTC+/PTC- splice variants were higher than in wild-type plants, the two CBC mutants investigated showed no change in the PTC+/PTC- ratios. Our results suggest that neither CBP20 nor CBP80 is involved in NMD in A. thaliana.
- Źródło:
-
Acta Biochimica Polonica; 2008, 55, 4; 825-828
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł