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    Wyświetlanie 1-5 z 5
    Tytuł:
    Układ typu ciecz/ciecz jako alternatywna metoda hodowli przestrzennej komórek adherentnych
    Liquid/liquid culture system as an alternative method of adherent cells 3-D culture
    Autorzy:
    Brzezińska, M.
    Grabowska, I.
    Dąbkowska, K.
    Pilarek, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2071710.pdf
    Data publikacji:
    2012
    Wydawca:
    Stowarzyszenie Inżynierów i Techników Mechaników Polskich
    Tematy:
    układ hodowlany ciecz/ciecz
    hodowla komórkowa
    kule zarodkowe
    mysie zarodkowe komórki macierzyste
    liquid/liquid culture system
    animal cell culture
    cell spheres
    Opis:
    Porównano wzrost mysich zarodkowych komórek macierzystych w postaci kul zarodkowych w hodowli standardowej (stała powierzchnia pokryta żelatyną) oraz w układzie ciecz/ciecz (perfluorodekalina/pożywka). Wykazano, że hodowla kul zarodkowych w układzie dwóch niemieszających się faz ciekłych pozwala znacznie wydłużyć żywotność agregatów mysich komórek macierzystych oraz prawdopodobnie utrzymać je w stanie niezróżnicowanym.
    The growth of mouse embryonic stem cell spheres cultured in the typical (on solid surface) and in liquid/liquid systems (perfluorodecalin/medium) has been compared. It has been showed that the liquid/liquid culture system enables one to extend the viability of cultured cells and probably maintains undifferentiated state of the mouse embryonic stem cell spheres.
    Źródło:
    Inżynieria i Aparatura Chemiczna; 2012, 4; 101-102
    0368-0827
    Pojawia się w:
    Inżynieria i Aparatura Chemiczna
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Enhanced Chondrocyte Proliferation in a Prototyped Culture System with Wave-Induced Agitation
    Autorzy:
    Pilarek, M.
    Godlewska, K.
    Kuźmińska, A.
    Wojasiński, M.
    Dąbkowska, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/184988.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    wave-induced agitation
    small-scale animal cell culture
    CP5 chondrocytes
    fibrousbased
    scaffold
    single-use bioreactor
    wzbudzanie falowe
    hodowla komórek zwierzęcych
    Chondrocyty CP5
    szafot
    bioreaktor
    Opis:
    One of the actual challenges in tissue engineering applications is to efficiently produce as high of number of cells as it is only possible, in the shortest time. In static cultures, the production of animal cell biomass in integrated forms (i.e. aggregates, inoculated scaffolds) is limited due to inefficient diffusion of culture medium components observed in such non-mixed culture systems, especially in the case of cell-inoculated fiber-based dense 3D scaffolds, inside which the intensification of mass transfer is particularly important. The applicability of a prototyped, small-scale, continuously wave-induced agitated system for intensification of anchorage-dependent CP5 chondrocytes proliferation outside and inside three-dimensional poly(lactic acid) (PLA) scaffolds has been discussed. Fibrous PLA-based constructs have been inoculated with CP5 cells and then maintained in two independent incubation systems: (i) non-agitated conditions and (ii) culture with wave-induced agitation. Significantly higher values of the volumetric glucose consumption rate have been noted for the system with the wave-induced agitation. The advantage of the presented wave-induced agitation culture system has been confirmed by lower activity of lactate dehydrogenase (LDH) released from the cells in the samples of culture medium harvested from the agitated cultures, in contrast to rather high values of LDH activity measured for static conditions. Results of the proceeded experiments and their analysis clearly exhibited the feasibility of the culture system supported with continuously wave-induced agitation for robust proliferation of the CP5 chondrocytes on PLA-based structures. Aside from the practicability of the prototyped system, we believe that it could also be applied as a standard method offering advantages for all types of the daily routine laboratory-scale animal cell cultures utilizing various fiber-based biomaterials, with the use of only regular laboratory devices.
    Źródło:
    Chemical and Process Engineering; 2017, 38, 2; 321-330
    0208-6425
    2300-1925
    Pojawia się w:
    Chemical and Process Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Liquid Perfluorochemicals as Flexible and Efficient Gas Carriers Applied in Bioprocess Engineering: An Updated Overview and Future Prospects
    Autorzy:
    Pilarek, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/185200.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    ciecz perfluorochemiczna
    fluorowęgiel
    gaz ciekły
    rozpuszczalność
    hybrydowy system hodowli
    liquid perfluorochemical
    fluorocarbon
    liquid gas carrier
    solubility
    cell/tissue culture
    hybrid culture system
    Opis:
    Fully synthetic, biochemically inert and water-immiscible liquid perfluorochemicals (PFCs) are recognised as flexible liquid carriers/scavengers of gaseous compounds (respiratory gases mainly, i.e. O2 and CO2) and increasingly applied in bioprocess engineering. A range of unmatched physicochemical properties of liquid PFCs, i.e. outstanding chemo- and thermostability, extremely low surface tension, simultaneous hydro- and lipophobicity, which result from carbon chain substitution with fluorine atoms (the most electronegative chemical element) and the presence of intramolecular C–F bonds (the strongest single bond known in organic chemistry) have been described in detail. Exceptional propensity to solubility of respiratory gases in liquid perfluorinated compounds has been widely discussed. Advantages and disadvantages of bioprocess applications of liquid PFCs in the form of a pure PFC as well as in an emulsified form have been pointed out. A liquid PFC-mediated mass transfer intensification in various types of microbial, plant cell and animal cell culture systems: from miniaturised microlitre-scale cultures, via biomaterial-based scaffolds containing culture systems, to litre-scale bioreactors, has been reviewed and elaborated on bearing in mind the benefits of bioprocesses.
    Źródło:
    Chemical and Process Engineering; 2014, 35, 4; 463-487
    0208-6425
    2300-1925
    Pojawia się w:
    Chemical and Process Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Modelling of a hybrid culture system with a stationary layer of liquid perfluorochemical applied as oxygen carrier
    Autorzy:
    Pilarek, M.
    Dąbkowska, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/185054.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    liquid perfluorochemical (fluorocarbon)
    liquid gas carrier
    mathematical modelling
    cell/tissue culture
    hybrid culture system
    ciecz perfluorochemiczna (fluorowęglowa)
    gaz ciekły
    modelowanie matematyczne
    hybrydowy system hodowli
    Opis:
    A mathematical model of a hybrid culture system supported with a stationary layer of liquid perfluorochemical (PFC) as a source of O2 for cells which grow in the aqueous phase of culture medium has been developed and discussed. The two-substrate Monod kinetics without inhibition effects, i.e. the Tsao-Hanson equation, has been assumed to characterise the biomass growth. The Damköhler number which relates the growth rate to the mass transfer effects has been used to appraise the regime (i.e. diffusion-limited or kinetics) of the whole process. The proposed model predicted accurately previously published data on the submerged batch cultures of Nicotiana tabacum BY-2 heterotrophic cells performed in a culture system supported with a stationary layer of hydrophobic perfluorodecalin as a liquid O2 carrier. Estimated values of the parameters of the model showed that the process proceeded in the kinetics regime and the growth kinetics, not the effects of the mass transfer between aqueous phase and liquid PFC, had essential influence on the growth of biomass.
    Źródło:
    Chemical and Process Engineering; 2016, 37, 1; 149-158
    0208-6425
    2300-1925
    Pojawia się w:
    Chemical and Process Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Growth of animal cells on flexible interface of liquid fluorocarbon
    Autorzy:
    Pilarek, M.
    Grabowska, I.
    Powiązania:
    https://bibliotekanauki.pl/articles/283970.pdf
    Data publikacji:
    2012
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    perfluorochemical (perfluorocarbon)
    liquid/liquid culture system
    flexible interfacial area
    animal cell aggregates
    Opis:
    The aim of this work was to study BHK-21 fibroblasts cultured on the interfacial area of two immiscible liquids: perfluorodecalin (hydrophobic; PFD) and cell culture medium (aqueous; DMEM) what allows creating 3-D multicellular structures. We showed that the robust growth of 3-D aggregated animal cells could be achieved on flexible PFD/DMEM interfacial area. We also indicated that 3-D aggregates of BHK-21 cells could be successfully subcultured on solid hydrophobic surface and cells could migrate from those multicellular structures, spread on solid surface and further grow in typical monolayered form. Results of our experiments showed that the PFD/medium system is simple and ready-to-use method without need of any inserts traditionally used for 3-D cultures of animal cells. Formed multicellular 3-D aggregates could be directly used in the same culture system for inoculation of biomaterial elements or scaffolds.
    Źródło:
    Engineering of Biomaterials; 2012, 15, 114; 12-14
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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