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Wyszukujesz frazę "plant tissue culture" wg kryterium: Temat

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    Wyświetlanie 1-4 z 4
    Tytuł:
    Methods of eliminating contaminations in tissue cultures on the example of selected ornamental plant species
    Autorzy:
    Parzymies, M.
    Dabski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/951204.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    tissue culture
    contamination
    ornamental plant
    Clematis
    biological contamination
    micropropagation
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of explants type and orientation on growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitro
    Autorzy:
    Kozak, D.
    Parzymies, M.
    Świstowska, A.
    Marcinek, B.
    Ismael, B.S.
    Powiązania:
    https://bibliotekanauki.pl/articles/12663898.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    Brazilian jasmine
    Mandevilla sanderi
    ornamental plant
    flowering plant
    pot plant
    commercial plant
    plant growth
    plant development
    plant regeneration
    explant type
    multiplication
    decapitation
    defoliation
    in vitro culture
    tissue culture
    cultivation experiment
    Opis:
    Mandevilla sanderi is an important commercial ornamental pot plant. Traditional vegetative propagation is limited due to the low rate, therefore there is a need to develop an alternative, more efficient method. There is an interest in development of micropropagation technology for the species, as it allows to obtain a lot of offsprings in a relatively short time. The aim of the present work was to estimate an influence of explants type and position on regeneration of Mandevilla sanderi in tissue culture. Four different types of explants (leafy shoot tips, decapitated leafy shoot tips, defoliated shoot tips, decapitated and defoliated shoot tips) were used in the experiment, which were placed on the media vertically, while defoliated shoot tips were placed horizontally or vertically upside down. The explants were cultivated on a Murashige and Skoog (MS) medium supplemented with 1 mg·dm–3 benzyladenine (BA) and 0.5 mg·dm–3 indole-3-butyric acid (IBA). It was noted that both explants orientation and positioning, influenced the multiplication rate. Defoliated shoot tips placed horizontally were characterized by higher multiplication rate (6.8) in comparison to upside down vertical positioning (3.2). It was also observed that removal of shoot apex improved axillary branching, while defoliation of shoots placed in a normal position reduced multiplication rate.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 4; 111-119
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The use of nano-silver for disinfection of Pennisetum alopecuroides plant material for tissue culture
    Autorzy:
    Parzymies, M.
    Pudelska, K.
    Poniewozik, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/12683367.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    Pennisetum alopecuroides
    grass species
    ornamental grass
    shoot
    explant
    plant material
    tissue culture
    plant growth regulator
    micropropagation
    contamination
    plant regeneration
    silver
    nanoparticle
    disinfection
    Opis:
    Initiation of tissue culture of many plant species is a very difficult stage due to appearance of many contaminations. The other problem might be a choice of media for regeneration. Initiation of grass species tissue cultures are thought to be very difficult. Therefore, a research was undertaken to evaluate the use of nano-silver particles for plant material disinfection and to estimate a medium Pennisetum alopecuroides. The plant material were buds and nodal explants that were disinfected in 2% NaOCl for 30 min or 0.1% HgCl2 for 1 min. Half of the explants disinfected with NaOCl were soaked in 50, 100 or 250 mg·dm–3 Ag NPs for 1 hour. Explants not soaked in nano-silver were placed on media with Ag NPs at concentrations of 4, 8 or 16 mg·dm–3. An influence of growth regulators on Pennisetum alopecuroides was evaluated in vitro. Regenerated shoots were placed on MS media with: 3 mg·dm–3 BA + 0.3 mg·dm–3 IBA, 3 mg·dm–3 KIN + + 0.3 mg·dm–3 IAA, 1 mg·dm–3 BA + 0.1 mg·dm–3 IBA. It was observed that the use of nano-silver particles lowered the level of contamination. The best results were obtained when Ag NPs was used at concentration of 100–250 mg·dm–3 alone or as a supplementation of the media, at concentration of 4 mg·dm–3 for nodes and 16 mg·dm–3 for adventitious buds. The use of nodal explants allowed to obtain less contamination. Regeneration depended on a media content. The most regenerated shoots were obtained on the MS media supplemented with 1 mg·dm–3 BA and 0.1 mg·dm–3 IBA.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 3; 127-135
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of growth regulators on dahlia propagation in tissue culture and acclimatization of plants in ex vitro conditions
    Autorzy:
    Marcinek, B.
    Parzymies, M.
    Poniewozik, M.
    Kozak, D.
    Durlak, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/12307379.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    Dahlia
    Dahlia x cultorum
    dahlia
    ornamental plant
    flowering plant
    tuberous plant
    tuberous root
    plant acclimatization
    growth regulator
    cytokinin
    gibberellic acid
    micropropagation
    tissue culture
    ex vitro method
    microcutting
    Opis:
    The aim of the work was to evaluate the influence of cytokinins on in vitro propagated dahlia and their consequent effect on acclimatization. Plant material consisted of shoot tips and nodes. Among the three cytokinins, benzyladenine, kinetin and 2-isopentenyl-adenine, only BA effectively stimulated the shoot multiplication from axiliary buds. The highest multiplication rate was obtained from nodes in the presence of 0.25– 0.5 mg·dm–3 BA. Higher concentrations shortened the internodes and decreased the leaf blades and growth of callus. 1 mg·dm–3 of KIN and 2iP positively influenced the shoot growth and size of leaves. Gibberellic acid (GA3) used with BA increased the number of auxillary shoots. The best quality shoots and the highest multiplication rate were obtained when 2 mg·dm–3 BA was used with 5 mg·dm–3 GA3. Cytokinins affected the rooting and acclimatization ex vitro. Dahlia shoots multiplicated in the presence of 1 mg·dm–3 KIN or 2iP rooted faster in the soil and 100% survived in field, while those from 1 mg·dm–3 BA media rooted slowly, had shorter shoots and only 60% of them survived. Plants bloomed after 11–12 weeks in the field. Dahlia plants that had been multiplicated in the presence of KIN had larger diameter and fresh weight in the field. BA and 2iP positively influenced the flower diameter, length of flower stalk and a number of the first-order shoots.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 5; 49-61
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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