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    Wyświetlanie 1-3 z 3
    Tytuł:
    Micro- and nanopatterned surfaces for guided adhesion, growth and phenotypic maturation of cells
    Autorzy:
    Bacakova, L.
    Filova, E.
    Grausova, L.
    Vandrovcova, M.
    Parizek, M.
    Novotna, K.
    Svorcik, V.
    Vacik, J.
    Rypacek, F.
    Kromka, A.
    Heitz, J.
    Shard, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/284586.pdf
    Data publikacji:
    2009
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    surface patterning
    microstructure
    nanostructure
    biofunctionalization
    endothelial cells
    vascular smooth muscle cells
    bone cells
    Opis:
    Micropatterned surfaces were created by UV light-irradiation of polytetrafluoroethylene through a metallic mask, by successive plasma polymerization of acrylic acid and 1,7-octadiene, or by creation of prominences and grooves by deposition of fullerenes C60 through a metallic mask. All these surface types were capable of inducing regionally-selective adhesion, proliferation and phenotypic maturation of vascular endothelial cells, vascular smooth muscle cells or human bone-derived MG 63 cells. Nanopatterned surfaces created by tethering GRGDSG oligopeptides through polyethylene oxide chains on a polymeric surface promoted spreading, formation of focal adhesion plaques and DNA synthesis in vascular smooth muscle cells. Surfaces nanopatterned with nanocrystalline diamond gave good support for the adhesion, growth and metabolic activity of osteoblast-like MG 63 cells.
    Źródło:
    Engineering of Biomaterials; 2009, 12, no. 89-91; 18-21
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Vascular smooth muscle cells in cultures on low density polyethylene modified with plasma discharge and biofunctionalization
    Autorzy:
    Parizek, M.
    Kasalkova, N.
    Bacakova, L.
    Kolarova, K.
    Lisa, V.
    Svorcik, V.
    Powiązania:
    https://bibliotekanauki.pl/articles/285087.pdf
    Data publikacji:
    2009
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    Ar plasma discharge
    biomaterials
    low-density polyethylene
    cell adhesion
    cell proliferation
    grafting
    tissue engineering
    vascular smooth muscle cells
    Opis:
    Low density polyethylene (LDPE) was modified by an Ar plasma discharge and then grafted with glycine (Gly), bovine serum albumin (BSA) or polyethylene glykol (PEG). Some plasma-treated samples and samples grafted with BSA were exposed to a suspension of colloidal carbon particles (C, BSA+C). Pristine LDPE and tissue culture polystyrene dishes (PSC) were used as control samples. The materials were seeded with rat aortic smooth muscle cells and incubated in a medium DMEM with 10% of fetal bovine serum. On day 1 after seeding, the cells on LDPE modified with plasma only, Gly, BSA and BSA+C adhered in similar numbers as on PSC, while the values on non-modified and PEG-modified samples were significantly lower. On day 5, the highest cell numbers were found again on LDPE with Gly, BSA and BSA+C. On day 7, the highest number of cells was found on LDPE modified only with plasma. The latter cells also dis-played the largest cell spreading area. The increased cell colonization was probably due to the formation of oxygen-containing chemical functional groups after plasma irradiation, and also due to positive effects of grafted Gly, BSA and BSA in combination with colloidal C particles.
    Źródło:
    Engineering of Biomaterials; 2009, 12, no. 89-91; 25-28
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Improved adhesion and growth of vascular smooth muscle cells in cultures on polyethylene modified by plasma discharge
    Autorzy:
    Parizek, M.
    Kasalkova, N.
    Bacakova, L.
    Kolarova, K.
    Lisa, V.
    Svorcik, V.
    Powiązania:
    https://bibliotekanauki.pl/articles/284295.pdf
    Data publikacji:
    2007
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    biomateriały
    inżynieria tkankowa
    Ar plasma discharge
    high density and low density polyethylene
    cell adhesion
    cell proliferation
    vascular smooth muscle cells
    biomaterials
    tissue engineering
    Opis:
    The attractiveness of synthetic polymers for cell colonization can be affected by physical and chemical modification of the polymer surface. In this study, high density polyethylene (HDPE, m.w. 0.952g/cm3) and low density polyethylene (LDPE, m.w. 0.922g/cm3) were modified by an Ar plasma discharge using Balzers SCD 050 device (exposure time 10, 50, 150 and 400 seconds, discharge power 1.7W). The material was then seeded with rat aortic smooth muscle cells (RASMC; passages 8 to 9, 17 000 cells/cm3) and incubated in a DMEM medium with 10% of fetal calf serum. On day 1 after seeding, the number of initially adhered cells was significantly higher on all modified HDPE and LDPE samples. On day 2, this difference persisted in HDPE, whereas in LDPE only the values on the samples modified by 150 and 400 seconds were significantly higher. On the 5th and 7th day, there were no significant differences in cell number among all LDPE samples. However, on the HDPE foils, significant differences were still apparent on the samples modified for 400 seconds. The cell spreading areas measured on day 1 after seeding were significantly larger on all modified LDPE samples, and, on day 2, on the HDPE samples exposed for 150s. The increased cell colonization was probably due to the formation of oxygen-containing chemical functional groups in the polymer. These results suggest that the responsiveness of the cell to the changes in physiochemical surface properties was more pronounced in HDPE than in LDPE. On both types of polyethylene, the most appropriate exposure time for the enhancement of cell adhesion and growth seemed to be 150 and 400 seconds.
    Źródło:
    Engineering of Biomaterials; 2007, 10, no. 67-68; 1-4
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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