- Tytuł:
- Novel luminescent dyes for confocal laser scanning microscopy used in Trematoda parasite diagnostics
- Autorzy:
-
Kirilova, Elena
Kecko, Sanita
Mežaraupe, Ligita
Gavarāne, Inese
Pučkins, Aleksandrs
Mickeviča, Ilona
Rubeniņa, Ilze
Osipovs, Sergejs
Bulanovs, Andrejs
Pupiņš, Mihails
Kirjušina, Muza - Powiązania:
- https://bibliotekanauki.pl/articles/1038378.pdf
- Data publikacji:
- 2018
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
benzanthrone dyes
trematode
confocal laser scanning microscopy - Opis:
- Benzanthrone derivates are now widely used in many industrial and scientific applications as dyes for polymers and textiles. In biochemical, biomedical and diagnostics investigations benzanthrone dyes are used as a lipophilic fluorescent probe since many benzanthrone derivates demonstrate bright fluorescence and they have ability to intercalate between membrane lipids. The aim of research presented here was to assess the luminescence ability of benzanthrone derivatives using microscopic visualization of biological objects. Accordingly, specimens of freshwater trematodes: Diplostomum spathaceum, Diplodiscus subclavatus and Prosotocus confusus, were stained by novel benzanthrone dyes using different fixatives. The samples were examined under a confocal laser scanning microscope. All of the dyes tested demonstrated good results for digestive and reproductive system visualization. Based on obtained results we conclude that benzanthrone dyes could be used for internal and external structure confocal laser scanning microscopic imaging of trematode specimens.
- Źródło:
-
Acta Biochimica Polonica; 2018, 65, 3; 449-454
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł