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Tytuł:
Stan zdrowotny i zróżnicowanie genetyczne buka zwyczajnego w Nadleśnictwie Siewierz na podstawie analiz chloroplastowego DNA
Health condition and genetic differentiation level of beech in the Siewierz Forest District assessed with cpDNA markers
Autorzy:
Nowakowska, J.A.
Oszako, T.
Powiązania:
https://bibliotekanauki.pl/articles/1013561.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
zroznicowanie genetyczne
cpDNA zob.DNA chloroplastowy
drzewa zdrowe
podatnosc na choroby
czynniki genetyczne
lesnictwo
Phytophthora
drzewa chore
Nadlesnictwo Siewierz
czynniki chorobotworcze
grzyby
buk zwyczajny
fytoftoroza
stan zdrowotny drzew
DNA chloroplastowy
Fagus sylvatica
drzewa lesne
fagus sylvatica
chloroplast dna markers
heterozygosity level
phythopthora spp.
Opis:
Beech decline phenomenon has been observed in Poland since the 1980s. The reasons are still unclear but the discovery of pathogens genus Phytophthora shed a new light on it. Stems as well as roots of beech trees were severely affected by P. citricola and P. cambivora. In order to find out the genetic background of infection, three loci of chloroplast microsatellite DNA were investigated in the genetic differentiation study of beeches growing in the Siewierz Forest District in Poland. Parameters of genetic diversity (h Nei) and differentiation (GST) in the chloroplast genome were estimated and compared between healthy and damaged trees. Healthy beeches were more heterozygous (h=0.243) than the damaged ones (h=0.113), and the distribution of cpDNA alleles was different between these two groups. Healthy trees were nearly 50% more genetically diversed than declining ones.
Źródło:
Sylwan; 2008, 152, 09; 11-20
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zamieranie olszy czarnej Alnus glutinosa (L.) Gaertn. wzdłuż rzeki Narewki na terenie Nadleśnictwa Białowieża
Decline of Black Alder Alnus glutinosa (L.) Gaertn. along the Narewka River in the Białowieża Forest District
Autorzy:
Malewski, T.
Topor, R.
Nowakowska, J.A.
Oszako, T.
Powiązania:
https://bibliotekanauki.pl/articles/2142614.pdf
Data publikacji:
2020
Wydawca:
Instytut Badawczy Leśnictwa
Tematy:
alder
dieback
drought
Phytophthora
pcr
olsza
zamieranie
susza
Opis:
Black Alder Alnus glutinosa (L.) Gaertn. is an important tree commonly growing in Poland. Alders are actinorhizal plants that play an important ecological role in riparian ecosystems through atmospheric nitrogen fixation, filtration and purification of waterlogged soils as well as providing a refuge for terrestrial and aquatic organisms thus helping to stabilize stream banks. Black Alder used to be considered a very pest and disease resistant species but, the situation changed in 2000, when an unprecedented decline of Alders was observed in Poland. In the Białowieża Forest District, this decline has been observed on wet meadow habitats and along rivers or watercourses. Currently, there are several hypotheses explaining Alder dieback, among them climatic changes and Phytophthora infections. In terms of climate, Black Alder requires a high atmospheric humidity during all phases of its reproductive cycle. It tolerates neither long-term summer flooding nor a significant decrease in the groundwater level. In terms of pests, oomycete pathogens of the genus Phytophthora are the most destructive plant pathogens known and many of them are present in forests and nurseries all over Europe. The aim of this study was to evaluate the health of Black Alder along the Narewka River in the Białowieża Forest District. Selected areas were monitored in 2012 and 2018, but no relationship between drought and alder health was found. A preliminary analysis of soil and water samples by real time PCR revealed the presence of two Phytophthora species: P. alni and P. cactorum. Further and more detailed research is required to elucidate the role of these pathogens in Alder dieback.
Źródło:
Leśne Prace Badawcze; 2020, 81, 4; 147-152
1732-9442
2082-8926
Pojawia się w:
Leśne Prace Badawcze
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nawozy fosforynowe jako stymulatory wzrostu roślin w szkółkach leśnych
Phosphite fertilizers as a plant growth stimulators in forest nurseries
Autorzy:
Tkaczyk, M.
Nowakowska, J.A.
Oszako, T.
Powiązania:
https://bibliotekanauki.pl/articles/991118.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
szkolki lesne
nawozy
Actifos
stymulatory wzrostu
sadzonki
sosna zwyczajna
Pinus sylvestris
swierk pospolity
Picea abies
dab szypulkowy
Quercus robur
buk zwyczajny
Fagus sylvatica
olsza czarna
Alnus glutinosa
wzrost roslin
fertilizer
actifos
seedlings
pinus sylvestris
picea abies
quercus robur
fagus sylvatica
alnus glutinosa
Opis:
In the situation of limited number of pesticides being approved for use in forest nurseries it is necessary to examine new products available on European market that stimulate growth and improve resilience and vitality of seedlings as a part of intgrated plant protection program. Paper presents test of fertilizer Actifos used in seven Polish nurseries in order to stimulate the growth of seedlings. In 64% of cases, Actifos stimulated increament of shoots (significantly for oaks, beeches, pines, spruces and alders) as well as for roots (significantly only for alders and oaks).
Źródło:
Sylwan; 2014, 158, 01; 3-9
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Plant bio-stimulator fertilizers can be applied in integrated plant management (IPM) in forest nurseries
Autorzy:
Tkaczyk, M.
Nowakowska, J.A.
Oszako, T.
Powiązania:
https://bibliotekanauki.pl/articles/38586.pdf
Data publikacji:
2015
Wydawca:
Instytut Badawczy Leśnictwa
Źródło:
Folia Forestalia Polonica. Series A . Forestry; 2015, 57, 4
0071-6677
Pojawia się w:
Folia Forestalia Polonica. Series A . Forestry
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Environmental risk assessment of genetically modified plants (GMO) - challenges and approaches
Autorzy:
Nowakowska, J.A.
Sulkowska, M.
Oszako, T.
Powiązania:
https://bibliotekanauki.pl/articles/38783.pdf
Data publikacji:
2014
Wydawca:
Instytut Badawczy Leśnictwa
Tematy:
environment risk
assessment
genetically modified plant
public health
alien species
pest
invasion
Źródło:
Folia Forestalia Polonica. Series A . Forestry; 2014, 56, 2
0071-6677
Pojawia się w:
Folia Forestalia Polonica. Series A . Forestry
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Charakterystyka genetyczna PCR-RFLP oraz ocena zdrowotnosci wybranych populacji debu elblaskiego i krotoszynskiego
Genetic characterization in relation to the health state of oak populations in the Elblaski i Krotoszynski regions of Poland
Autorzy:
Nowakowska, J.A.
Oszako, T.
Bieniek, J.
Rakowski, K.
Powiązania:
https://bibliotekanauki.pl/articles/45956.pdf
Data publikacji:
2007
Wydawca:
Instytut Badawczy Leśnictwa
Tematy:
dab elblaski
dab krotoszynski
zmiennosc genetyczna
populacje roslin
ekotypy
Quercus robur
lesnictwo
zdrowotnosc drzew
DNA chloroplastowy
dab szypulkowy
metoda PCR-RFLP
drzewa lesne
Źródło:
Leśne Prace Badawcze; 2007, 3; 33-51
1732-9442
2082-8926
Pojawia się w:
Leśne Prace Badawcze
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molekularna diagnostyka wybranych patogenów z rodzaju Phytophthora w ramach integrowanej ochrony roślin
Molecular diagnostic of Phytophthora pathogens as a tool for Integrated Pest Management
Autorzy:
Nowakowska, J.A.
Malewski, T.
Tereba, A.
Borys, M.
Oszako, T.
Powiązania:
https://bibliotekanauki.pl/articles/989551.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
fitopatologia
Phytophthora
wykrywanie
identyfikacja
Phytophthora alni subsp.multiformis
Phytophthora lacustris
Phytophthora taxon hungarica
metody badan
metoda real time PCR
sondy TaqMan
real time pcr
taqman
butt and fine root pathogens
phytophthora
Opis:
Traditional detection methods such as baiting or direct isolation take a long time and are incapable to handling large volume of material to be tested. The real−time PCR−based techniques are faster, more sensitive, more easily automated, and do not require post−amplification procedures. Species−specific primers for Phytophthora were designed based on the internal transcribed spacer regions (ITS) of rDNA collected from the NCBI DNA database. Primers and probes were designed using the Allele ID 7 at default search criteria. Specific probes were labeled with the reporter dyes JOE (6−carboxy−4,5−dichloro−2,7−dimethoxyfluorescein) at the 5' end and HBQ1 quencher at the 3' end (Sigma−Aldrich). The specificity of primers and fluorogenic probes was tested against genomic DNA of P. alni subsp. multiformis, P. lacustris and P. taxon hungarica. The real−time PCR reactions with the specific probes and primers yielded positive results with five concentrations of standards obtained by standard PCR reaction for corresponding Phytophthora species. The negative control (lack of DNA pathogens) yielded no amplification products. Standard curves showed a linear correlation between input DNA and cycle threshold (Ct) values with R² from 0.994 (P. alni) to 0.998 (P. taxon hungarica). The amplification efficiency of target DNA varied from 94.6% (P. alni) to 100% (P. taxon hungarica). The validation of the primers and probes designed for analysed Phytophthora species was performed on pure cultures, on soil samples from the forest nursery and declining oak stands. The designed probes displayed the high specificity of the detection of investigated species in pure cultures. The presented new molecular TaqMan probes can fully assist the integrated pest management as a powerful tool for a quick detection of above pathogenic organisms in forest nurseries. The molecular detection of harmful phytophthoras and in consequences diminishing of fungicides use for their control in forestry fully support European Union directives as well as the ‘Good plant protection practice measures' elaborated by European and Mediterranean Organisation of Plant Protection.
Źródło:
Sylwan; 2016, 160, 05; 365-370
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Multipleksowa detekcja gatunków Phytophthora przy użyciu platformy Fluidigm
Multiplex detection of Phytophthora spp. using the Fluidigm platform
Autorzy:
Sikora, K.
Oszako, T.
Kubiak, K.
Nowakowska, J.A.
Malewski, T.
Powiązania:
https://bibliotekanauki.pl/articles/2142612.pdf
Data publikacji:
2020
Wydawca:
Instytut Badawczy Leśnictwa
Tematy:
forest soil
oomycetes
oak dieback
pathogens
next generation sequencing
gleby leśne
oomycety
zamieranie dębów
patogeny
sekwencjonowanie nowej generacji
Opis:
The genus Phytophthora plays an important role not only in agriculture but also in forest ecosystems. As the number of known Phytophthora species continues to grow, identifying new isolates in this genus has become increasingly challenging even by DNA sequencing. Therefore, the development of proper techniques for detection and identification is crucial for monitoring and control of these pathogens in the forestry sector. In recent years, new molecular methods using innovative approaches have indeed been developed. However, the majority of these methods was designed to detect single Phytophthora species. Techniques that are able to target multiple species would offer advantages, especially for the assessment of Phytophthora diversity in the environment. This paper describes a multiplex assay for the identification of eight Phytophthora isolates, down to the species level, based on a Fluidigm platform employing pyrosequencing. The obtained results showed that for an accurate determination of the species, it is sufficient to know the sequence of two markers, ITS and COX1. The sensitivity of this test is sufficient to identify Phytophthora in a pure culture. Unfortunately, analysis based on a pyrosequencing platform does not provide enough data to simultaneous identify multiple Phytophthora species in samples collected in the field. This problem could be resolved in the future by sequencing using more efficient platforms like Illumina or IonTorrent.
Źródło:
Leśne Prace Badawcze; 2020, 81, 4; 161-166
1732-9442
2082-8926
Pojawia się w:
Leśne Prace Badawcze
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zmienność genetyczna naturalnego odnowienia i drzewostanu macierzystego sosny (Pinus sylvestris L.) i świerka (Picea abies L. Karst.)
Genetic variability of Scots pine (Pinus sylvestris L.) and Norway spruce (Picea abies L. Karst.) natural regeneration compared with their maternal stands
Autorzy:
Nowakowska, J.A.
Zachara, T.
Konecka, A.
Powiązania:
https://bibliotekanauki.pl/articles/1206918.pdf
Data publikacji:
2014
Wydawca:
Instytut Badawczy Leśnictwa
Tematy:
lesnictwo
drzewostany macierzyste
odnowienia naturalne
drzewa lesne
sosna zwyczajna
Pinus sylvestris
swierk pospolity
Picea abies
zmiennosc genetyczna
Opis:
The genetic variability and biodiversity of tree populations ensure the stability and sustainability of forest ecosystems. New research tools based on molecular DNA markers enable precise characterisation of forest genetic resources, i.e. detection of different allele frequencies in mature trees and progeny populations. The paper describes the genetic structure of mature stands of Scots pine (Pinus sylvestris L.) in Oława Forest District and Norway spruce (Picea abies L. Karst.) in Jawor Forest District and stands of their respective progeny. In the Scots pine stand, there was a slight increase (0.6%) in heterozygosity level and a larger increase (4.9%) in the inbreeding coefficient of progeny trees. In the Norway spruce stand, despite a small reduction (0.9%) in heterozygosity, a similar increase (4.6%) in the inbreeding coefficient of progeny was revealed. In both stands, allele richness and the partition probability of basic clustering were high. Both pine and spruce adults and progeny trees were characterised by high levels of genetic similarity (96% and 79%, respectively). Gene flow between the mature and progeny populations was high (Nm > 1) for both Scots pine and Norway spruce. Conservation of the gene pool within forest tree stands requires an increase in the proportion of natural regeneration. To estimate the extent to which genes are transmitted between adult trees and their progeny, more studies are needed, especially taking into account the influence of silviculture measures, like selective tree cutting, on the genetic variability of the younger generation. These results confirm that the gene pool was conserved when transmitted between the stands studied, as well as highlight the usefulness of such a study for silvicultural purposes.
Źródło:
Leśne Prace Badawcze; 2014, 75, 1; 47-54
1732-9442
2082-8926
Pojawia się w:
Leśne Prace Badawcze
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Monitorowanie chorób wywołanych przez patogeniczne lęgniowce za pomocą analiz DNA
Monitoring of diseases caused by pathogenic oomycetes using DNA analysis
Autorzy:
Nevoigt, F.
Oszako, T.
Nowakowska, J.A.
Powiązania:
https://bibliotekanauki.pl/articles/1008933.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
fitopatologia
czynniki chorobotworcze
grzyby
legniowce
Oomycetes
choroby grzybowe
monitoring
Phytophthora
Pythium
diagnostyka molekularna
analiza DNA
metoda real time PCR
phytophthora spp.
real time pcr
its−dna molecular diagnostics
baiting
rhododendrons
Opis:
Phytophthora species are best known as pathogens of agricultural crops (e.g. P. infestans), but there are also invasive pathogens destroying forest atands (e.g. P. ramorum in the USA) or even whole forest ecosystems (e.g. P. cinnamomi in Australia). Still, little is known about indiginous species, especially in wild ecosystems. Rhododendrons are well known as a reservuar for oomycetes’ development. The main objectives of the present study were to develop the new tool for the identification of pathogenic Phytophthora and Phytium based on DNA sequence analysis of the ITS1, 5.8S gene and ITS2 region. Rhododendrons leaves served as specific plant baits. In order to reach the goal the real time PCR, the nested PCR and the DNA sequencing of the rDNA ITS region were carried out. The genomic DNA was extracted form the symptomatic rhododendron leaves. Three distinct Oomycetes species: Phytophthora cactorum, Pythium mercuriale and em>Pythium recalcitrans were detected in rhododendron leaves and registered in GenBank.
Źródło:
Sylwan; 2010, 154, 07; 450-455
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zmiany w strukturze genetycznej naturalnego odnowienia dębu (Quercus petraea [Matt.] Liebl.) w odniesieniu do drzew matecznych
Changes in genetic structure of sessile oak (Quercus petraea [Matt.] Liebl.) natural regeneration in relation to maternal trees
Autorzy:
Nowakowska, J.A.
Michalska, A.
Zachara, T.
Powiązania:
https://bibliotekanauki.pl/articles/990023.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
drzewa lesne
badania genetyczne
dab bezszypulkowy
Quercus petraea
odnowienia naturalne
struktura genetyczna
markery DNA
drzewa potomne
drzewa mlode
zroznicowanie genetyczne
drzewa mateczne
zmiennosc genetyczna
ssr markers
genetic differentiation
natural regeneration
Opis:
The genetic variability of sessile oak (Quercus petraea L.) mature stand and its natural progeny was investigated. Comparison between genetic structure of parental and progeny trees was based on frequencies of nuclear microsatellite (SSR) alleles occurring in three DNA loci. A slight (4%) increase of gene pool between oak mature and progeny trees was revealed by heterozygosity level estimation, maintaining 86.3% of genetic similarity between generations. Also allele richness, partition probability of basic clustering and inbreed coefficient proved the high genetic similarity between parental and progeny of investigated oak trees. The gene flow occurred within the stands as far as rare alleles were transmitted or new ones appeared in the progenies. The results highlight the necessity of such a study for silvicultural measures taken in order to proceed natural or artificial regeneration in forest tree stand.
Źródło:
Sylwan; 2014, 158, 02; 83-89
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wykorzystanie związków fosforynowych w leśnictwie
The use of phosphates in forestry
Autorzy:
Tkaczyk, M.
Kubiak, K.A.
Sawicki, J.
Nowakowska, J.A.
Oszako, T.
Powiązania:
https://bibliotekanauki.pl/articles/45658.pdf
Data publikacji:
2016
Wydawca:
Instytut Badawczy Leśnictwa
Tematy:
fosforyny
nawozy
fungicydy
biostymulatory
zastosowanie
lesnictwo
Źródło:
Leśne Prace Badawcze; 2016, 77, 1
1732-9442
2082-8926
Pojawia się w:
Leśne Prace Badawcze
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 27

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