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    Wyświetlanie 1-2 z 2
    Tytuł:
    Application of DNA markers against illegal logging as a new tool for the Forest Guard Service
    Autorzy:
    Nowakowska, J.A.
    Powiązania:
    https://bibliotekanauki.pl/articles/38611.pdf
    Data publikacji:
    2011
    Wydawca:
    Instytut Badawczy Leśnictwa
    Tematy:
    application
    DNA marker
    DNA structure
    wood
    molecular identification
    Forest Guard Service
    tree species
    determination
    DNA profile
    polymerase chain reaction
    Opis:
    DNA markers are currently the most precise tool for forest tree species identification and can be used for comparative analyses of plant material. Molecular diagnosis of evidence and reference material is based on comparing the structure of DNA markers duplicated in the PCR reaction and estimation of the DNA profiles obtained in studied wood samples. For this purpose, the microsatellite DNA markers are the most suitable tool because of their high polymorphism and accurate detection of structural changes in the genome. The analysis of tree stump DNA profiles let avoid timely collection of data such as tree age, diameter, height and thickness, although such a piece of information may advantageous in wood identification process. For each examined tree species, i.e. Pinus sylvestris L., Picea abies (L.) Karst., Quercus robur L. and Q. petraea (Matt.) Liebl., Fagus sylvatica L., Betula pendula L., and Alnus glutinosa L., wood identification was possible via the DNA profiles established on a basis of minimum 4 microsatellite nuclear DNA loci, and at least one cytoplasmatic (mitochondrial or chloroplast) DNA marker. Determination of the DNA profiles provided fast and reliable comparison of genetic similarity between material of evidence (wood, needles, leaves, seeds) and material of reference (tree stumps) in the forest. This was done with high probability (approximately 98– 99%).
    Źródło:
    Folia Forestalia Polonica. Series A . Forestry; 2011, 53, 2
    0071-6677
    Pojawia się w:
    Folia Forestalia Polonica. Series A . Forestry
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Pochodzenie drzewostanów świerkowych (Picea abies L. Karst.) z Puszczy Białowieskiej na tle regionu RDLP Białystok na podstawie analiz mitochondrialnego DNA
    Origin of the Norway spruce (Picea abies L. Karst.) stands from Bialowieza Forest at the level of Bialystok Regional Directorate of the State Forests based on mitochondrial DNA analysis
    Autorzy:
    Nowakowska, J.A.
    Łukaszewicz, J.
    Borys, M.
    Tereba, A.
    Konecka, A.
    Zawadzka, A.
    Sułkowska, M.
    Zajączkowski, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/987121.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    lesnictwo
    Puszcza Bialowieska
    drzewostany swierkowe
    drzewa lesne
    swierk pospolity
    Picea abies
    markery genetyczne
    DNA mitochondrialny
    pochodzenie roslin
    zmiennosc genetyczna
    RDLP Bialystok
    genetic diversity
    mitochondrial dna marker
    postglacial migration routes
    białowieża forest
    Opis:
    Considered to be the most natural of all other Polish stands Picea abies in Białowieża Forest has suffered severely of bark beetle damages for decades. In order to find out some historical events in native Norway spruce stands diversity at regional level, we carried out the study on poly− morphism with DNA markers and performed Principal Coordinate Analysis (PCoA) on the basis of genetic distance matrix. Two ranges (i.e. Baltico−Nordic and Hercyno−Carpathian) of Norway spruce cover north−eastern and southern Poland, respectively. The spruceless zone separates the these two ranges in lowland Poland. From genetic point of view, the Baltico−Nordic P. abies populations mainly harbor mitochondrial haplotype ‘c', whereas the spruces from the Hercyno− −Carpathian range – haplotype ‘a'. Until recently, the Białowieża Forest has been considered to belong to the northern range of spruce occurrence in Europe. Seven populations from Biało− wieża Forest were studied (two of them from the Strict Reserve in Białowieski National Park) according to mitochondrial DNA diversity, and compared with genetic diversity found in 24 other populations located in Białystok Regional Directorate of the State Forests (north−eastern Poland). All studied spruce populations were more than 100 years old and were of natural origin. As result, the spruces from the Białowieża Forest harbored three mitochondrial haplotypes of the nad1 gene (‘c', ‘a' and ‘a1'), with the haplotype ‘a' being more frequently present (>51%) than the hap− lotype ‘c'. The spruce stands from Białowieża Forest have the highest gene diversity (h Nei=0.527) comparing to the other stands from the Białystok RDSF. The PCoA proved the particularity of the spruces from Białowieża Forest grouping them into one cluster of genetic similarity. Our data demonstrated that most of Norway spruces populations from Białystok RDSF harbor haplotype ‘c', which confirms their historical relationship with the Baltico−Nordic range of P. abies in Europe, while most spruces from Białowieża Forest have different historic origin, because they share high frequency of the southern haplotype ‘a'. It also turned out that Norway spruce pop− ulations from two different European ranges met in lowland Poland after the last glacial period, as proved by the presence of two mitochondrial haplotypes ‘a' and ‘c'. It can be assumed, that the spruceless zone was created by human activity in the past millennia. The results of all molecular analyses confirmed the unique character of spruces of Białowieża Forest, which is distinguished by the greater richness of the gene pool in comparison to the region of Białystok RDSF as well as good adaptation to local environmental conditions.
    Źródło:
    Sylwan; 2017, 161, 01; 40-51
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-2 z 2

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