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    Wyświetlanie 1-3 z 3
    Tytuł:
    Test mikrojądrowy w retrospektywnej dozymetrii biologicznej
    Micronucleus assay in rapid retrospective biological dosimetry
    Autorzy:
    Rawojć, K.
    Miszczyk, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/134746.pdf
    Data publikacji:
    2016
    Wydawca:
    ADVSEO
    Tematy:
    micronuclei
    micronucleus assay
    retrospective biological dosimetry
    ionizing radiation
    cell death
    Opis:
    Rapid retrospective biological dosimetry allows absorbed dose evaluation post exposure to ionizing radiation. One of the main tools of biodosimetry is based on the analysis of the effects resulting from the impact of ionizing radiation on the cell. Various cytogenetic tests give possibility of the accurate dose estimation. To investigate cell response to radiation one performs the analysis of biomarkers approved by International Atomic Energy Agency e.g. the analysis of dicentric chromosomes or micronuclei frequency. Micronucleus test is relatively a faster and therefore more effective method to study changes in the genetic material, induced by various genotoxic agents. This study confirms that micronulei frequency and nuclear division index analysis allows for appropriate absorbed dose estimation when it comes to ionizing radiation. In order to further optimize and facilitate the micronucleus assay and other cytogenetic tests in rapid retrospective biological dosimetry, the research are still ongoing.
    Źródło:
    Technical Issues; 2016, 4; 78-82
    2392-3954
    Pojawia się w:
    Technical Issues
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of 60 MeV Protons and 250 kV X-Rays on Cell Viability
    Autorzy:
    Miszczyk, J.
    Panek, A.
    Rawojć, K.
    Swakoń, J.
    Prasanna, P.
    Rydygier, M.
    Gałaś, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/1398817.pdf
    Data publikacji:
    2016-02
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    87.50.-a
    87.53.-j
    87.53.Bn
    Opis:
    Particle radiotherapy such as the one using proton beams, provides a successful treatment approach in many cancer types. However, the cellular and molecular mechanisms by which proton irradiation induces cell death, particularly in a human peripheral blood lymphocyte model has not been examined in detail. Comparative studies of the biological effects, such as cell death, of particle therapy versus conventional X-rays treatment are of utmost importance. Here, we compared the viability of human peripheral blood lymphocyte following in vitro irradiation with protons (therapeutic 60 MeV proton beam) and photon beam (250 kV, X-rays), by applying separate doses within the range of 0.3-4.0 Gy. Cell viability was assessed 1 and 4 h after irradiation with protons and X-rays by the FITC-Annexin V labelling procedure (Apoptotic & Necrotic & Healthy Cells Quantification Kit, Biotium). Results showed that irradiation with both radiation types reduced the number of viable cells in a dose-dependent manner, as assessed as a function of the duration of post-irradiation time. Protons proved more fatal to the cells treated than X-ray photons. This demonstrates a difference in cell viability after irradiation with protons and photons in a human peripheral blood lymphocyte model.
    Źródło:
    Acta Physica Polonica A; 2016, 129, 2; 222-225
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparison of Methods in Studies of Cell Death Mechanisms
    Autorzy:
    Borkowska, A.
    Nowakowski, M.
    Miszczyk, J.
    Lipiec, E.
    Wiltowska-Zuber, J.
    Rawojć, K.
    Kwiatek, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/1030322.pdf
    Data publikacji:
    2018-02
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    growth and division
    cell processes
    fluorescence
    optimization
    Opis:
    While studying the influence of ionizing radiation or certain chemical agents on cells, it is crucial to not only determine cytotoxicity, but also to follow cell death mechanisms. There are different methods to screen processes of cell death and still very important question remains unanswered about differences in results that could be caused by various experimental steps in procedures. Based on literature review two protocols of cell death determination were compared. First protocol regarded collecting cells floating in medium before trypsinization and following centrifugation of them. In the second protocol floating cells were discarded and attached ones were stained and fixed. In all experiments three different untreated cell lines (A172, DU145 as cancer cell lines and in comparison, fibroblasts (FB CCL 110), as a non- cancerous cell line) were used to test applied protocols. Cells were cultured and death processes were examined at different time points up to 120 h. Compared protocols showed statistically significant differences, especially in terms of necrosis, which was higher when included floating cells from culture medium and then centrifuging them. Therefore, presented results show importance of choosing a valid experimental procedure in case of evaluating cells viability and types of cell death pathways quantitatively.
    Źródło:
    Acta Physica Polonica A; 2018, 133, 2; 263-266
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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