- Tytuł:
- Effect of low - energy laser irradiation and antioxidant supplementation on cell apoptosis during skeletal muscle post - injury regeneration in pigs
- Autorzy:
-
Otrocka-Domagala, I.
Mikolajczyk, A.
Pazdzior-Czapula, K.
Gesek, M.
Rotkiewicz, T.
Mikiewicz, M. - Powiązania:
- https://bibliotekanauki.pl/articles/30603.pdf
- Data publikacji:
- 2015
- Wydawca:
- Polska Akademia Nauk. Czytelnia Czasopism PAN
- Opis:
- The aim of this study was to evaluate the effect of low-energy laser irradiation, coenzyme Q10 and vitamin E supplementation on the apoptosis of macrophages and muscle precursor cells during skeletal muscle regeneration after bupivacaine-induced injury. The experiment was conducted on 75 gilts, divided into 5 experimental groups: I – control, II – low-energy laser irradiation, III – coenzyme Q10, IV – coenzyme Q10 and vitamin E, V – vitamin E. Muscle necrosis was induced by injection of 0.5% bupivacaine hydrochloride. The animals were euthanized on subsequent days after injury. Samples were formalin fixed and processed routinely for histopathology. Apoptosis was detected using the TUNEL method. The obtained results indicate that low-energy laser irradiation has a beneficial effect on macrophages and muscle precursor cell activity during muscle post-injury regeneration and protects these cells against apoptosis. Vitamin E has a slightly lower protective effect, limited mainly to the macrophages. Coenzyme Q10 co-supplemented with vitamin E increases the activity of macrophages and muscle precursor cells, myotube and young muscle formation. Importantly, muscle precursor cells seem to be more sensitive to apoptosis than macrophages in the environment of regenerating damaged muscle.
- Źródło:
-
Polish Journal of Veterinary Sciences; 2015, 18, 3
1505-1773 - Pojawia się w:
- Polish Journal of Veterinary Sciences
- Dostawca treści:
- Biblioteka Nauki
Artykuł