- Tytuł:
- Surface Coat Differences between Invasive Entamoeba histolytica and Non-Invasive Entamoeba dispar
- Autorzy:
-
González-Robles, A.
CHÁVEZ-MUNGUÍA, Bibiana
SALAZAR‑VILLATORO, Lizbeth
LAGUNES-GUILLÉN, Anel
HERNÁNDEZ-RAMÍREZ, Verónica Ivonne
TALAMÁS‑ROHANA, Patricia
MARTÍNEZ-PALOMO, Adolfo - Powiązania:
- https://bibliotekanauki.pl/articles/763748.pdf
- Data publikacji:
- 2015
- Wydawca:
- Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
- Tematy:
- Amebiasis, Entamoeba histolytica, Entamoeba dispar, Cell surface, Concanavalin A.
- Opis:
- Using ultrastructural cytochemical techniques we have found differences in the distribution of surface coat components between the invasive protozoan parasite Entamoeba histolytica and the non-invasive Entamoeba dispar. Carbohydrate-containing components and anionic sites in the cell surface of both species were detected by staining with ruthenium red and cationized ferritin, respectively. Ruthenium red staining revealed a thicker surface coat in E. histolytica trophozoites, whereas trophozoites of E. dispar showed a higher concentration of cationized ferritin particles on its surface. Mannose or glucose residues were found at the plasma membrane of both parasites treated with Concanavalin A (Con A)-peroxidase; the surface reaction product was more evident in E. dispar, compared with E. histolytica. Con A rapidly produced surface caps in E. histolytica trophozoites, whereas E. dispar showed a much less efficient mobilization of surface Con A receptors. Agglutination with Con A produced much larger clumps in E. histolytica in comparison with E. dispar. In turn, biotinylation assays revealed striking differences in the composition of surface membrane proteins in both amebic species. Overall, these results further emphasize the phenotypic differences between these two common parasites of the human intestinal tract, once considered to be the same protozoan.
- Źródło:
-
Acta Protozoologica; 2015, 54, 3
1689-0027 - Pojawia się w:
- Acta Protozoologica
- Dostawca treści:
- Biblioteka Nauki
Artykuł