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Wyświetlanie 1-15 z 85
Tytuł:
Isolation and genetic analysis of a variant porcine epidemic diarrhea virus in China
Autorzy:
Li, R.-f.
Tian, X.-g.
Liu, Y.
Xu, J.
Liu, D.-y.
Powiązania:
https://bibliotekanauki.pl/articles/30492.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Porcine epidemic diarrhea virus (PEDV) is having a severe effect on the pig breeding industry in central China. The mucosa and the content of the small intestine from newborn pre-weaned piglets with diarrhea were tested for the presence of PEDV by molecular and morphologic methods, and found to be positive. Negative-staining electron microscopy (EM) revealed the presence of coronavirus- like particles in the samples. The result of molecular detection by nested RT-PCR based on the amplification of the M gene was positive. Using a novel alternative method we successfully propagated the PEDV strain (CH/QX-2) in Vero cells, confirmed by ultrathin sections of the cells and Immunofluorescence assay (IFA). Phylogenetic analysis based on the partial S gene showed that the CH/QX-2 isolate was genetically closer to strains more commonly found in China, but differed genetically from two domestic strains (CH/S, 1986 and LZC, 2007), Korean strains (DR13, 2007), and the vaccine strain (CV777 vs) currently being used in China. CH/QX-2 formed a unique clade in the derived phylogenetic tree indicating that the CH/QX-2 strain currently circulating in central China is a new variant of PEDV. This study extends current knowledge on the diversity and epidemiology of PEDV.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Research on a New Synthesis of LLM-105 Using N-Nitroso-bis(cyanomethyl)amine
Autorzy:
Jing, S.
Liu, Y.
Liu, D.
Guo, J.
Powiązania:
https://bibliotekanauki.pl/articles/358371.pdf
Data publikacji:
2016
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
LLM-105
energetic materials
synthesis
characterization
properties
Opis:
A new three-step synthetic method was designed to prepare 2,6-diamino- 3,5-dinitropyrazine 1-oxide (LLM-105) with an overall yield of 43.5%, using iminodiacetonitrile, triethylamine and hydroxylamine hydrochloride as the starting materials, and involved nitrosylation, cyclization and nitration. The structure of LLM-105 was characterized by FTIR, 1H NMR spectroscopy and elemental analysis. The crucial factors and mechanism of the cyclization were explored. Thermal decomposition, mechanical sensitivity and the purity of the LLM-105 were determined by TG-DTA, an impact sensitivity instrument and high performance liquid chromatography (HPLC) respectively. The results showed that LLM-105 synthesised via 2,6-diamino-3,5-dinitropyrazine (ANPZ) was less sensitive to impact, but the LLM-105 synthesized by the new method had some obvious advantages in terms of purity, security and production costs. Moreover, the LLM-105 synthesized by this new method can be initiated reliably without recrystallization.
Źródło:
Central European Journal of Energetic Materials; 2016, 13, 1; 21-32
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of experimental infections of various Tembusu virus strains isolated from geese, ducks and chickens on ducklings
Autorzy:
Xu, T.
Huang, X.
Li, Y.
Liu, Q.
Liu, X.
Han, K.
Liu, Y.
Yang, J.
Zhao, D.
Bi, K.
Sun, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087719.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
goose-derived
duck-derived
chicken-derived
Tembusu virus
pathogenicity
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 2; 389-396
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Generation and characterization of a monoclonal antibody against duck Tembusu virus envelope protein
Autorzy:
Han, K.
Zhao, D.
Liu, Y.
Liu, Q.
Huang, X.
Yang, J.
Bi, K.
Xu, T.
Li, Y.
Powiązania:
https://bibliotekanauki.pl/articles/31495.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused massive economic losses to the duck industry in China. Envelope (E) protein of DTMUV is an important structural protein, which is able to induce protective immune response in target animals and can be used as specific serological diagnosis tool. In this study, a novel monoclonal antibody, designated mAb 3E9, was generated against DTMUV E protein. It is positive in indirect ELISA against both His-E protein and the purified whole viral antigen. Also, this mAb showed positive reaction with DTMUV in Western blot and indirect immunofluorescence assay, and the isotype was IgG1. End-point neutralizing assay performed in BHK-21 cells revealed that the neutralization titer of 3E9 against DTMUV JS804 strain reached 1:50. Furthermore, functional studies revealed that 3E9 blocks infection of DTMUV at a step on viral attachment. The anti-E mAbs produced in the present work may be valuable in developing an antigen-capture ELISA test for antigen detection or a competitive ELISA test for antibody detection or therapeutic medicine for DTMUV in poultry.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Toluene Mono-nitration in a Semi-batch Reactor
Autorzy:
Chen, L. P.
Chen, W. P.
Liu, Y.
Peng, J. H.
Liu, R. H.
Powiązania:
https://bibliotekanauki.pl/articles/358853.pdf
Data publikacji:
2008
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
toluene mono-nitration
apparent activation energy
semi-batch reaction
thermal hazard
Opis:
Toluene mono-nitration in a semi-batch reactor was investigated with reaction calorimeter (RC1). The mixed acid HNO3/H2SO4/H2O (wt%) was used in proportion 13/66/21, which is similar to industrial parameters. The exothermic rates at different reaction temperatures were compared, and then the curves of heat generation rate after dosing were analyzed. The Maximal Temperature attainable by runaway of the desired Synthetic Reaction (MTSR) under different conditions were calculated for the course hazard evaluation. The results showed that the average reaction heat of mono-nitration was between 169.07~177.11 kJ mol-1, and the special heat of reactant was about 2 kJ kg-1 K-1. Average Ea of second order kinetic was about 30 kJ mol-1, and reaction rate was 10-4 mol s-1 l-1 order of magnitude when the strength of sulphuric acid was about 73.5%. It was also found that when temperature or stirring speed increases, the potential heat accumulation decreases.
Źródło:
Central European Journal of Energetic Materials; 2008, 5, 2; 37-47
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Investigation on Irreversible Expansion of 1,3,5-Triamino-2,4,6-trinitrobenzene Cylinder
Autorzy:
Sun, J.
Kang, B.
Zhang, H.
Liu, Y.
Xia, Y.
Yao, Y.
Liu, X.
Powiązania:
https://bibliotekanauki.pl/articles/357936.pdf
Data publikacji:
2011
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
TATB
irreversible expansion
XRD
lattice parameters
Opis:
In this study, the irreversible expansion of TATB cylinder is investigated. No evident variation of the lattice parameters is observed on TATB crystal. The density of TATB powder decreases by only about 0.02% after it suffered from thermal cycling process at the range from -54 C to 74 C, while the density of TATB cylinder decreases by about 1.0%. It is suggested that the density variation of TATB powder has little contribution to the density decrease of TATB cylinder. Therefore, the increasing interstices between TATB powder originated from the thermal cycling should be responsible to the irreversible expansion of TATB cylinder.
Źródło:
Central European Journal of Energetic Materials; 2011, 8, 1; 69-79
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dynamic characteristics of magnetic coupling in horizontal axis wave energy device
Autorzy:
Zhang, J.
Liu, Y.
Liu, J.
He, T.
Xie, Y.
Powiązania:
https://bibliotekanauki.pl/articles/258488.pdf
Data publikacji:
2017
Wydawca:
Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
Tematy:
wave energy
magnetic coupling
dynamic characteristics
transfer function
step response
steady state error
Opis:
To solve the dynamic response problems of magnetic coupling in the horizontal axis wave energy device, this has researched the dynamic characteristicsof magnetic coupling. The fitting formula about torque and angle of the magnetic coupling is obtained through experiments. The mathematical models of the magnetic coupling torque transmission are established. The steady state error of the magnetic coupling and the transfer function of the output angle are obtained. The analytical solution of the step response of the output angle in time domain is derived. The influence of the torsional rigidity, the damping coefficient and the driven rotor’s rotational inertia on dynamic characteristics of the magnetic coupling isanalyzed. According to the analysis results, the design rules of magnetic coupling are proposed.
Źródło:
Polish Maritime Research; 2017, S 3; 165-170
1233-2585
Pojawia się w:
Polish Maritime Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A review of progress in current research on Chinese flowering cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee)
Autorzy:
Liu, W.
Liu, Y.
Kleiber, T.
Powiązania:
https://bibliotekanauki.pl/articles/1192785.pdf
Data publikacji:
2021
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
Tematy:
Chinese flowering cabbage
nutritional value
yield
breeding
Opis:
Chinese flowering cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) is an important leafy vegetable species, which originates from Guangdong province in southern China. There is a variety called purple Chinese flowering cabbage (Brassica campestris L. ssp. chinensis L. Makino var. purpurea Bailay), which is popular in the Yangtze River basin, e.g. in Wuhan and Sichuan provinces. This manuscript is a review of recent studies on the nutritional value, breeding methods and factors affecting the yield of Chinese flowering cabbage. This vegetable is rich in glucosinolates, polyphenols, amino acids, fatty acids, soluble sugar and vitamin C. It also exhibits some antioxidative activity, e.g. the TEAC value was 16.93 μmol Trolox g-1 and the FRAP value was 12.01 µmol Fe(II) g-1 in total. Controlling the Cd level in Chinese flowering cabbage is important due to the fact that it can easily accumulate Cd in its edible leaves and flowering stems. To reduce the effect given by Cd, foliar spray with trace elements had been studied. Moreover, nitrogen fertilizer, biofertilizer and several cultivation methods have been implemented to improve the yielding and nutritional value of Chinese flowering cabbage. Depending on the conditions, fertilisation increased the yield from 19.1% to 168%, whereas the fresh weight of a single plant varied from 0.835 kg to 1.683 kg and the average yielding in the field ranged from 7.857 t ha-1 to 19.1 t ha-1. In the last part of this paper, we reviewed the different breeding methods that have been used to develop new varieties of Chinese flowering cabbage meeting specific requirements, such as selecting a hybrid with kale to enrich the genotype, increase the resistance or discover a specific bolting gene. In brief, Chinese flowering cabbage has the potential for future research and commercial market.
Źródło:
Journal of Elementology; 2021, 26, 1; 149-162
1644-2296
Pojawia się w:
Journal of Elementology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Fluorescence Analysis as an Effective Method Used in Micro/Trace Explosive Detection
Autorzy:
Liu, Y.
Shu, Y.
Liu, Xue-yong.
Xiong, Y.
Zhong, Fa-chun.
Sun, Y.
Powiązania:
https://bibliotekanauki.pl/articles/358808.pdf
Data publikacji:
2009
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
micro/trace explosive detection
fluorescence analysis
conjugated polymer
sensing materials
Opis:
There are many kinds of explosives, and their detection methods vary. Nitroaromatic compound is one of the composition of the explosives commonly used. The fuorescence will be quenched when they touch the fuorescent conjugated polymers. General methods for explosives detection have been summarized in this paper, in addition the application of novel fuorescence analysis technique in explosives detection has been introduced. Fluorescent conjugated polymer as chemical sensing material for explosive detection has been reviewed in detail, also a novel fuorescent sensing flm self-assembled by fuorescent small molecule pyrene and homogeneous fuoroimmunoassay have been presented briefy. The development of fuorescence analysis used in the area of determining explosives has been prospected.
Źródło:
Central European Journal of Energetic Materials; 2009, 6, 3-4; 303-311
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthesis and Characterization of Metal Derivatives of 1,1,2,2-Tetranitraminoethane (TNAE)
Autorzy:
Zhu, C.
Meng, Z.
Xu, Z.
Liu, W.
Liu, Y.
Shi, X.
Xie, T.
Powiązania:
https://bibliotekanauki.pl/articles/358571.pdf
Data publikacji:
2018
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
Ionic compound
1,1,2,2-tetranitraminoethane
thermal property
safety performance
thermal decomposition kinetics
Opis:
The introduction of anions and cations into energetic materials can significantly improve their physicochemical and energetic properties. The K, Zn, Ba, Cu and Pb salts of 1,1,2,2-tetranitraminoethane (TNAE) were synthesized using TNAE as the starting material, and were characterized by IR spectroscopy, NMR spectroscopy and elemental analysis. All of the energetic salts exhibited relatively high thermal stabilities (decomposition temperatures ranging from 195.1 °C to 279.3 °C) and high densities (ranging from 2.1 g·cm–3 to 3.7 g·cm–3). It was demonstrated that the introduction of metal ions conveys better thermal stability than covalent TNAE itself. More importantly, the Zn, Ba and Cu salts exhibited good thermal stability, high density, low sensitivity, and therefore have the potential to be a new class of insensitive, highly energetic explosives for practical applications.
Źródło:
Central European Journal of Energetic Materials; 2018, 15, 3; 435-444
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
Autorzy:
Dai, G.
Huang, X.
Liu, Q.
Li, Y.
Zhang, L.
Han, K.
Yang, J.
Liu, Y.
Xue, F.
Zhao, D.
Powiązania:
https://bibliotekanauki.pl/articles/16647377.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
epitope
goose astrovirus
capsid protein
monoclonal antibody
Opis:
Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Production of Carbon Fibre Bulked Yarns by the Airflow Dispersion Method
Wytwarzanie przędz puszystych z włókien węglowych metodą dyspersji przepływu powietrza
Autorzy:
Zhao, X.
Liu, Y.
Liu, G.
Powiązania:
https://bibliotekanauki.pl/articles/233684.pdf
Data publikacji:
2017
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Biopolimerów i Włókien Chemicznych
Tematy:
carbon fibre
bulk yarn
development
properties
włókno węglowe
przędza puszysta
wytrzymałość na rozerwanie
nierównomierność
włochatość
Opis:
Using carbon fibre tows as raw materials, carbon fibre bulk yarns were producedby the airflow dispersion method for the first time. The breaking strength, strength irregularity, yarn irregularity and hairiness index of the carbon fibre bulk yarn were used as evaluation indices, and preparation technology for carbon fibre bulk yarn was optimized using the orthogonal experimental method. Subsequently the disordered structure of homemade carbon fibre bulk yarn, the ability to fix the resin, and the surface contact angle were investigated. Finally infrared spectral analysis of the carbon fibre bulk yarn was carried out. Results show that the best preparation technology for carbon fibre bulk yarn is as follows: nozzle air pressure 0.45 MPa, spinning speed 150 m/min, and nozzle diameter 2.2 mm. The degree of disorder of fibres of T700 carbon fibre bulk yarn fibre is 18.70%~25.60%; as the degree of disorder of carbon fibre bulk yarn increases, the ability to fix the resin is enhanced. The process of carbon fibre tows producing bulk yarns is a physical one.
W pracy wytworzono przędze puszyste z włókien węglowych metodą dyspersji przepływu powietrza, następnie zbadano ich wytrzymałość na rozerwanie, nierównomierność oraz włochatość. Przeprowadzono także spektralną analizę wiązki włókien węglowych w podczerwieni. Na podstawie otrzymanych wyników określono optymalne parametry wytwarzania przędz puszystych z włókien węglowych, tj.: ciśnienie powietrza w dyszy 0,45 MPa, prędkość wirowania 150 m/min i średnica dyszy 2,2 mm. Stopień nieuporządkowania włókien węglowych T700 wynosi 18,70 ~ 25,60%. Stwierdzono, że wraz ze wzrostem stopnia nieuporządkowania zwiększa się zdolność wiązania żywicy.
Źródło:
Fibres & Textiles in Eastern Europe; 2017, 6 (126); 34-40
1230-3666
2300-7354
Pojawia się w:
Fibres & Textiles in Eastern Europe
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 85

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