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    Wyświetlanie 1-4 z 4
    Tytuł:
    Generation and characterization of a monoclonal antibody against duck Tembusu virus envelope protein
    Autorzy:
    Han, K.
    Zhao, D.
    Liu, Y.
    Liu, Q.
    Huang, X.
    Yang, J.
    Bi, K.
    Xu, T.
    Li, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/31495.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused massive economic losses to the duck industry in China. Envelope (E) protein of DTMUV is an important structural protein, which is able to induce protective immune response in target animals and can be used as specific serological diagnosis tool. In this study, a novel monoclonal antibody, designated mAb 3E9, was generated against DTMUV E protein. It is positive in indirect ELISA against both His-E protein and the purified whole viral antigen. Also, this mAb showed positive reaction with DTMUV in Western blot and indirect immunofluorescence assay, and the isotype was IgG1. End-point neutralizing assay performed in BHK-21 cells revealed that the neutralization titer of 3E9 against DTMUV JS804 strain reached 1:50. Furthermore, functional studies revealed that 3E9 blocks infection of DTMUV at a step on viral attachment. The anti-E mAbs produced in the present work may be valuable in developing an antigen-capture ELISA test for antigen detection or a competitive ELISA test for antibody detection or therapeutic medicine for DTMUV in poultry.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of experimental infections of various Tembusu virus strains isolated from geese, ducks and chickens on ducklings
    Autorzy:
    Xu, T.
    Huang, X.
    Li, Y.
    Liu, Q.
    Liu, X.
    Han, K.
    Liu, Y.
    Yang, J.
    Zhao, D.
    Bi, K.
    Sun, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087719.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    goose-derived
    duck-derived
    chicken-derived
    Tembusu virus
    pathogenicity
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 2; 389-396
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
    Autorzy:
    Dai, G.
    Huang, X.
    Liu, Q.
    Li, Y.
    Zhang, L.
    Han, K.
    Yang, J.
    Liu, Y.
    Xue, F.
    Zhao, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647377.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    epitope
    goose astrovirus
    capsid protein
    monoclonal antibody
    Opis:
    Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Stability control of propeller Autonomous Underwater Vehicle based on combined sections method
    Autorzy:
    Qi, D.
    Feng, J.
    Liu, A.
    Hu, J.
    Xu, H.
    Li, Y.
    Ashraf, M. A.
    Powiązania:
    https://bibliotekanauki.pl/articles/259119.pdf
    Data publikacji:
    2015
    Wydawca:
    Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
    Tematy:
    propeller AUV
    dynamic model
    stability control
    simulation
    Opis:
    Learning from the motion principle of quadrotor, a symmetric propeller AUV, which has small size and low velocity is designed. Compared with the AUV equipped with rudders, it has better maneuverability and manipulation at low velocity. According to the Newton-Euler method, the 6 DOF kinematic model and dynamic model of the propeller AUV are established. A stability controller that consists of 3 different PID controllers is designed. It makes the depth and attitude angle as trigger conditions, and the relevant controller is chosen in different moving process. The simulation experiments simulate ideal motion state and disturbed motion state, and experiments results show that the stability controller based on combined sections method can make the best of mature technology of PID, and meet the control requirements in different stages. It has a higher respond speed and accuracy, improving the stability of the propeller AUV under the disturbance of complex ocean currents.
    Źródło:
    Polish Maritime Research; 2015, S 1; 157-162
    1233-2585
    Pojawia się w:
    Polish Maritime Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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