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Wyszukujesz frazę "Gal`chinsky, N.V." wg kryterium: Autor

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    Tytuł:
    The need for the application of modern chemical insecticides and environmental consequences of their use: a mini review
    Autorzy:
    Oberemok, V.V.
    Laikova, K.V.
    Zaitsev, A.S.
    Temirova, Z.Z.
    Gal'chinsky, N.V.
    Nyadar, P.M.
    Shumskykh, M.N.
    Zubarev, I.V.
    Powiązania:
    https://bibliotekanauki.pl/articles/66108.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    application need
    modern insecticide
    insecticide
    chemical insecticide
    environmental consequence
    DNA insecticide
    insect pest
    insecticide resistance
    plant protection
    Opis:
    Currently, the use of insecticides is an acute problem. Due to rapid population growth, the primary task is to increase food production. Beyond abiotic factors (drought, soil salinity, etc.) that reduce crop yields, farmers face problems with insect pests that can decrease crop productivity up to 60%. Also, insects are carriers of severe viral and protozoan human diseases. The need for application of insecticides is not questioned but many of them cause resistance of insect pests to them. This, in turn, leads to the necessity to invent new insecticides that are safe and more effective for long-term use. Preparations based on conservative parts of nucleic acids, particularly contact DNA insecticides, could be used to solve insecticide resistance problem as control agents which are well-tailored to target insect pests. This mini review is devoted to these issues.
    Źródło:
    Journal of Plant Protection Research; 2017, 57, 4
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Insecticidal activity of three 10–12 nucleotides long antisense sequences from 5.8S ribosomal RNA gene of gypsy moth Lymantria dispar L. against its larvae
    Autorzy:
    Oberemok, V.V.
    Laikova, K.V.
    Useinov, R.Z.
    Gal`chinsky, N.V.
    Novikov, I.A.
    Yurchenko, K.A.
    Volkov, M.E.
    Gorlov, M.V.
    Brailko, V.A.
    Plugatar
    Powiązania:
    https://bibliotekanauki.pl/articles/2084849.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    antisense oligonucleotides
    DNA insecticides
    gypsy moth
    insect pest control
    Lymantria dispar
    5.8S ribosomal RNA
    Opis:
    5.8S ribosomal RNA plays an important role in protein synthesis and eukaryotic ribosome translocation. Contact DNA insecticides based on antisense fragments of 5.8S ribosomal RNA gene of gypsy moth Lymantria dispar L. showed prospective insecticidal activity on its larvae. The most pronounced insecticidal effect was found for antisense fragments 10 and 11 nucleotides long (oligoRIBO-10 and oligoRIBO-11), whereas 12 nucleotides long fragment (oligoRIBO-12) caused the lowest level of insect mortality. This data corresponds to results obtained earlier using rabbit reticulocyte and wheat germ extracts, where maximum inhibition of protein synthesis was observed when a relevant oligomer 10-11 nucleotides long was used, whilst longer chain lengths resulted in reduced inhibition. Using oligoRIBO-11 fragment we have shown penetration of antisense oligonucleotides to insect cells through insects’ exoskeletons. MALDI technique registered the penetration of the oligoRIBO-11 fragment into insect cells after 30 min and a significant response of insect cells to the applied oligonucleotide after 60 min, which indicates not only that the oligonucleotide enters the insect cells, but also the synthesis of new substances in response to the applied DNA fragment. Contact DNA insecticides developed from the L. dispar 5.8S ribosomal RNA gene provide a novel biotechnology for plant protection using unmodified antisense oligonucleotides.
    Źródło:
    Journal of Plant Protection Research; 2019, 59, 4; 561-564
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-2 z 2

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