- Tytuł:
-
Metody molekularne stosowane w badaniu różnorodności mikroorganizmów glebowych
Molecular methods used in studies of diversity of the soil microorganisms - Autorzy:
-
Behnke-Borowczyk, J.
Kwaśna, H.
Belka, M. - Powiązania:
- https://bibliotekanauki.pl/articles/1006468.pdf
- Data publikacji:
- 2012
- Wydawca:
- Polskie Towarzystwo Leśne
- Tematy:
-
mikroorganizmy glebowe
identyfikacja
roznorodnosc biologiczna
metody badan
metody molekularne
ekstrakcja DNA
amplifikacja DNA
biodiversity
microbiota
molecular methods
soil - Opis:
- Application of the correct methods of study is essential for a proper description and understanding of natural communities of microorganisms and their relationships. Classical methods used for detection and identification of soil microorganisms resulted in underestimation of the microbiota. It has been claimed that classical methods, based mainly on morphology, led to the identification of only 1% of the soil microbiota, usually species that predominate in culture because of their fast growth and abundant sporulation. Molecular methods allow rapid, accurate, sensitive and cost−effective identification and enumeration of microorganisms. They are designed to replace and/or support classical approaches. This review summarizes some of the current and emerging nucleic acid−based molecular approaches used for detection, discrimination and quantification of microbes in the environment, mostly in soil.
- Źródło:
-
Sylwan; 2012, 156, 04; 294-304
0039-7660 - Pojawia się w:
- Sylwan
- Dostawca treści:
- Biblioteka Nauki
Artykuł