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    Wyświetlanie 1-3 z 3
    Tytuł:
    The influence of selected techniques of bovine leukocyte isolation on their viability and metabolism
    Autorzy:
    Urban-Chmiel, R.
    Lisiecka, U.
    Chlopas, A.
    Kurek, L.
    Wernicki, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/30570.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The aim of the study was to assess the effect of selected isolation methods on the viability and metabolism of bovine leukocytes. The cells were isolated using a Ficoll 1077, Histopaque 1083 gradient and osmotic shock method, and Ficoll or Histopaque with osmotic shock. Evaluation were made of the total number of cells, viability after isolation and in 24h culture on RPMI 1640 medium and metabolism with NBT reduction assay. Microscopic and cytometric evaluation of the leukocytes revealed that the isolation methods applied had an influence on their number and viability. Based on the results it can be concluded that isolation methods of cells in a Histopaque or Ficoll yield highly pure cell fractions with high viability.
    Źródło:
    Polish Journal of Veterinary Sciences; 2011, 14, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Copper and iron deficiency in dairy cattle
    Autorzy:
    Abramowicz, B.
    Kurek, L.
    Chalabis-Mazurek, A.
    Lutnicki, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/1192794.pdf
    Data publikacji:
    2021
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
    Tematy:
    haematology
    copper and iron deficiency
    dairy cows
    Opis:
    Copper deficiency is a common homeostatic disorder in dairy cattle farms in Poland. A simultaneous decrease in blood copper and iron concentrations is very rarely diagnosed and described in dairy cows. Copper is necessary for the mobilization of iron from the liver and its transport to bone marrow, where it is used for erythropoiesis. The aim of the study was to observe changes in haematological parameters in cows, resulting from copper deficiency or simultaneous deficiency of copper and iron, in comparison with animals from the same herds in which no deficiencies of these micronutrients were found. The study was conducted on 56 cows of the HF breed, aged from 3 to 6 years, from 3 dairy farms. The animals selected for the study were divided into three groups, two deficiency groups and one control group – without deficiency. Group I comprised animals with reduced concentration of copper in the serum, while group II comprised animals with low copper and iron concentrations. Group III comprised healthy cows without clinical symptoms and mineral deficiency. In both deficiency groups, the one characterized by a low concentration of copper, as well as the one with low concentrations of copper and iron, a reduction in red blood cell parameters, which was statistically significant versus the control group, was observed. In the examined animals, normocytic normochromic anaemia was found in group I, while normocytic hypochromic anaemia was diagnosed in group II. Cows with simultaneous Cu and Fe deficiency have worse milk yield results and they are more often culled from the herd. It is not clear why only some animals in a herd develop simultaneous deficiency of these micronutrients.
    Źródło:
    Journal of Elementology; 2021, 26, 1; 241-248
    1644-2296
    Pojawia się w:
    Journal of Elementology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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