- Tytuł:
- Optical properties of (3-(acetamidomethyl)phenyl)boronic acid and its interactions with selected sugars
- Autorzy:
-
Kur, K.
Przybyt, M.
Miller, E. - Powiązania:
- https://bibliotekanauki.pl/articles/410414.pdf
- Data publikacji:
- 2018
- Wydawca:
- Politechnika Łódzka. Wydawnictwo Politechniki Łódzkiej
- Tematy:
-
(3-(Acetamidomethyl)phenyl)boronic acid
quenching constant
pK
lifetime
3AAPBA
stała wygaszania
trwałość - Opis:
- (3-(Acetamidomethyl)phenyl)boronic acid (3AAPBA)has at pH 7 absorbance maximum at 270 nm with molar absorbance coefficient 516 M-1cm-1. 3AAPBA exhibits weak fluorescence with maximum at 297 nm and quantum yield 0.062 ± 0.001. Fluorescence decay is monoexponential and the lifetime is 2.05 ± 0.01 ns. Interactions of 3AAPBA with selected sugars were studied by absorbance, steady-state and time-resolved fluorescence measurements. At pH 7 fluorescence of 3AAPBA is quenched only by fructose (with quenching constant 67.9 M-1) and to some extend by galactose. Addition of these two monosaccharides causes also changes of absorbance spectra of 3AAPBA. Acid-base dissociation of free 3AAPBA and its esters with sugars was studied by absorbance and steady-state fluorescence measurements in pH range from 4.5 to 11.00. Esterification of phenylboronic acid derivatives by sugars leads to increased acidity of them. In case of 3AAPBA the obtained values of pK indicate that affinity of studied sugars towards it can be ordered as follows: fructose > galactose > glucose > maltose > lactose > sucrose. At pHs higher than pK the fluorescence decays turn to biexponential with additional shorter component in lifetime which we propose to attribute to anionic form of 3AAPBA or its esters.
- Źródło:
-
Biotechnology and Food Science; 2018, 82, 1; 15-27
2084-0136
2299-6818 - Pojawia się w:
- Biotechnology and Food Science
- Dostawca treści:
- Biblioteka Nauki
Artykuł