- Tytuł:
- Biomimetic alginate/perfluorocarbon microcapsules - tthe effect on in vitro metabolic activity and long-term cell culture
- Autorzy:
-
Stefanek, Agata
Kulikowska-Darłak, Aleksandra
Bogaj, Karolina
Nowak, Aleksandra
Dembska, Joanna
Ciach, Tomasz - Powiązania:
- https://bibliotekanauki.pl/articles/2173417.pdf
- Data publikacji:
- 2022
- Wydawca:
- Polska Akademia Nauk. Czytelnia Czasopism PAN
- Tematy:
-
alginian
mikrokapsułki
hodowla komórkowa
perfluorowęglowodory
metabolizm
alginate
microcapsules
cell culture
perfluorocarbons
metabolism - Opis:
- Cell encapsulation seems to be a promising tool in tissue engineering. However, it has been shown to have several limitations in terms of long-term cell cultures due to an insufficient oxygen supply. In this study we propose the use of novel microcapsules designed for long-term cell culture consisting of an alginate shell and perfluorocarbon (PFC) core, which works as a synthetic oxygen carrier and reservoir. The influence of PFC presence in the culture as well as the size of structures on cell metabolism was evaluated during 21-day cultures in normoxia and hypoxia. We showed significant improvement in cell metabolism in groups where cells were encapsulated in hydrogel structures with a PFC core. The cells maintained a typical metabolism (oxidative phosphorylation) through all 21 days of the culture, overcoming the oxygen supply shortage even in large structures (diameter ¡ 1 mm). Applying PFC in alginate matrices can improve cell metabolism and adaptation in long-term cell cultures.
- Źródło:
-
Chemical and Process Engineering; 2022, 43, 1; 81--95
0208-6425
2300-1925 - Pojawia się w:
- Chemical and Process Engineering
- Dostawca treści:
- Biblioteka Nauki
Artykuł