Autor: Krywult, M - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Method of measurement of nitrate reductase activity in field conditions
Autorzy:: Krywult, M.
Bielec, D
Powiązania:: https://bibliotekanauki.pl/articles/123113.pdf
Data publikacji:: 2013
Wydawca:: Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:: nitrate reductase
kinetic changes
field analysis methodology
optimization
Opis:: For the last three decades the interest in biomonitoring and ecological studies has been rapidly growing. Therefore, it was necessary develop of new methods of analysis for biochemical parameters which allow to quantify biological response of investigated organisms for environmental factors. The main goal of this paper demonstrates optimal conditions for enzyme kinetics analysis conducted in the field in situ. Nitrate reductase activity is typically assayed in vivo by measuring nitrite production in tissue which has been vacuum infiltrated with buffered nitrate solution. For this study a nitrate reductase assay was adapted from a number of studies with own modifications of authors. Leaves of examined plants were collected from the investigated plots and immediately placed into test tubes with buffer solution (potassium phosphate dibasic containing 0.6% propanol-1) and evacuated in 0.33 atm. for 10 minutes. Then, known amount of potassium nitrate was added, and the solution sample was analyzed in order to obtain a background level of nitrite. The foliage samples were incubated for 2 hours at 20 °C in darkness. Following this procedure, they were given the most optimal conditions for reaction stability. After incubation the amount of synthesized nitrite was determined colorimetrically using sulfanilamide and N-(1-naphthyl)ethylenediamine dihydrochloride, measured at 540 nm. The foliage samples were oven-dried to obtain dry mass. The level of nitrate reductase activity was calculated as the amount of nitrite produced in nmol per gram of dry mass of foliage tissue per hour. The result obtained during the research demonstrate the changes of nitrate reductase dynamics according to change of incubation parameters. Dynamics of enzyme activity with changes of solution pH and incubation temperature was presented. Installation for conducting infiltration process and construction of incubation chamber is also described in this paper.
Źródło:: Journal of Ecological Engineering; 2013, 14, 1; 7-11
2299-8993
Pojawia się w:: Journal of Ecological Engineering
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Skocz do pozycji: 2.

Tytuł:: Nitrate reductase activity (NRA) in the invasive alien Fallopia japonica: seasonal variation, differences among habitats types, and comparison with native species
Autorzy:: Chmura, D.
Krywult, M.
Kozak, J.L.
Powiązania:: https://bibliotekanauki.pl/articles/56983.pdf
Data publikacji:: 2016
Wydawca:: Polskie Towarzystwo Botaniczne
Opis:: Nitrate reductase activity (NRA) was studied in the invasive alien plant F. japonica (Japanese knotweed) during the vegetation season and among natural, semi-natural, and human-made habitats and compared with NRA in selected native species. NRA was measured directly in the field from the beginning of May until the beginning of October. NRA was much higher than in the plant’s native range, i.e., East Asia, and showed a high degree of variation over time with the highest values being reached at the stage of fast vegetative growth and at the beginning of fruiting. NRA was highest on dumping sites probably due to the high nitrogen input into soils and near traffic and the emission of NOx by vehicles. A comparison of the enzyme activity in four selected native plant species indicated that NRA in F. japonica was the highest with the exception of Urtica dioica, which exhibited a similar activity of the enzyme. A detailed comparison with this species showed that differences between these species on particular dates were influenced by differences in the phenology of both plants. The initial results that were obtained suggest that nitrogen pollution in an environment can contribute to habitat invasibility and a high level of NRA, which in addition to the many plant traits that are commonly accepted as characteristic of invasiveness features, may be an important factor that enhances invasion success.
Źródło:: Acta Societatis Botanicorum Poloniae; 2016, 85, 3
0001-6977
2083-9480
Pojawia się w:: Acta Societatis Botanicorum Poloniae
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Skocz do pozycji: 3.

Tytuł:: Nitrate reductase activity in chosen mosses: a case of study in skalny colliery waste tip
Aktywność reduktazy azotanowej u wybranych mchów na przykładzie hałdy powęglowej skalny
Autorzy:: Krywult, M.
Salachna, A.
Chmura, D.
Żarnowiec, J.
Powiązania:: https://bibliotekanauki.pl/articles/106622.pdf
Data publikacji:: 2013
Wydawca:: Towarzystwo Chemii i Inżynierii Ekologicznej
Tematy:: bryophytes
nitrogen fixation
environmental pollution
mszaki
wiązanie azotu
zanieczyszczenie środowiska
Opis:: Two species of mosses in relation to nitrogen metabolism were examined. This subject is little known in this group of plant. Investigations of nitrate reductase activity in green tissues of Brachythecium rutabulum (Hedw.) Schimp. and Atrichum undulatum (Hedw.) P.Beauv. were performed. The study was conducted in two localities: heavy contaminated waste tip Skalny located in Upper Silesia, and Blonia City Park in Bielsko-Biala which place was chosen as a control area. For both species high activity of the enzyme was detected. The nitrate reductase activity varied between 99 to 9093 nmol per g dry mass per hour for B. rutabulum and 265 to 5135 nmol per g d.m. per hour of nitrite synthesized for A. undulatum respectively on Skalny waste tip. In the control area the results varied between 747 to 1077 for B. rutabulum and 171 to 518 nmol per g d.m. per hour of nitrite synthesized for A. undulatum, respectively. The differences were statistically significant only between the two species but not between habitats probably due to high dispersion and small amount of replications. The levels of nitrate and nitrite in stream water in both areas were also measured. In the Skalny waste tip there were high and reached 1.66 mg dm-3 of nitrite and 65 mg dm-3 of nitrate, respectively. In the control area these amounts were lower and reach zero level for nitrite and 4.5 mg dm-3 of nitrate, respectively.
Dwa gatunki mchów badano pod kątem asymilacji azotu. To zagadnienie jest mało poznane u tej grupy roślin. Przeprowadzono badania aktywności reduktazy azotanowej w zielonych tkankach Brachythecium rutabulum (Hedw.) Schimp. i Atrichum undulatum (Hedw.) P.Beauv. Badania zostały wykonane w dwóch miejscach: na hałdzie powęglowej Skalny na Górnym Śląsku i w parku w dzielnicy Błonia w Bielsku-Białej, które zostało wybrane jako miejsce kontrolne. Dla obu gatunków stwierdzono wysoką aktywność enzymu. Aktywność reduktazy azotanowej wahała się od 99 do 9093 nmol na g suchej masy na godzinę dla B. rutabulum i 265 do 5135 nmol na g suchej masy na godzinę azotynu syntetyzowanego u A. undulatum na hałdzie Skalny. W miejscu kontrolnym wyniki wahały się od 747 do 1077 dla B. rutabulum i 171 do 518 nmol na g sm/h u A. undulatum. Różnice były statystycznie znaczące tylko pomiędzy gatunkami, ale nie pomiędzy typem siedliska prawdopodobnie ze względu na wysoką dyspersję i małą liczbę powtórzeń. Zmierzono także poziom azotanów i azotynów w strumieniu wody w obu miejscach. Na hałdzie Skalny zawartość była wysoka i osiągnęła odpowiednio: 1,66 mg dm-3 azotynu oraz 65 mg dm-3 azotanu. W miejscu kontrolnym wartości te były niższe i osiągnęły odpowiednio poziom zerowy dla azotynów i 4,5 mg dm-3 dla azotanu.
Źródło:: Chemistry-Didactics-Ecology-Metrology; 2013, 18, 1-2; 61-68
2084-4506
Pojawia się w:: Chemistry-Didactics-Ecology-Metrology
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Informacja

Wyszukujesz frazę "Krywult, M" wg kryterium: Autor

Źródło danych

Dostawca treści

Kolekcja

Rok wydania

Wydawca

Temat

Autor

Typ dokumentu

Język